Determining Rest Concentration of Diazinon in Agriculture Products (Melon And Cucmber) with GC-NPD and GC-MS Methods

Moalem, Mitra; Ghorbani, Ahmad; Adel Moalem, Seied; Balali, Mehdi; Solhi, Hassan

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Volume 1, Issue 2 (Summer 2007) IJT 2007, 1(2): 4-4 | Back to browse issues page

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Moalem M, Ghorbani A, Adel Moalem S, Balali M, Solhi H. Determining Rest Concentration of Diazinon in Agriculture Products (Melon And Cucmber) with GC-NPD and GC-MS Methods. IJT 2007; 1 (2) :4-4
URL: http://ijt.arakmu.ac.ir/article-1-29-en.html

Determining Rest Concentration of Diazinon in Agriculture Products (Melon And Cucmber) with GC-NPD and GC-MS Methods

Mitra Moalem¹

, Ahmad Ghorbani²

, Seied Adel Moalem³

, Mehdi Balali³

, Hassan Solhi ^*

⁴

1- MSc of Toxicology
2- Department of Toxicology, Ahvaz Medical University, Ahvaz,Iran
3- Department of Toxicology, Mashhad Medical University, Mashhad, Iran
4- Department of Toxicology, Arak Medical University, Arak, Iran , solhi2@yahoo.com

Abstract: (10585 Views)

Introduction: Diazinon is a moderately toxic broad-spectrum organophosphate, with a LD50 of 350 to 400 mg/kg for humans. Diazinon is absorbed through the skin and gastrointestinal tract and is rapidly metabolized within a few hours. Diazinon residues in melons and cucumbers have recently became a major health concern in Iran. Thus we decided to investigate Diazinon levels in melon and cucumbers. Method and Materials: Twenty cucumbers and 30 melons were randomly purchased from the market in Mashhad. The extraction procedure was in HR-P columns precondition with methanol and water. The subsequent elution of diazinon was accomplished by a mixture of hexane-ethyl acetate (1:1, v/v) prior to determination by GC-NPD and GC-MS/MS. Standards were prepared spiking blank juice samples to contract the observed matrix effect.
Results: Mean recovery rate for diazinon was 95.4% with relative standard deviation lower than %9 in a concentration range of 5-200ng/ml. Mean and SD of diazinon in melons was 107.64±38.5 ng/kg. Diazinon was not detected in cucumber samples. It was later confirmed that diazinon was not used for the crops of these cucumbers.
Conclutions: GC-NPD and MS/MS was developed to determine residues of diazinon in melons and cucumbers. The GC-MS/MS analytical method showed a high efficacy for determination of dizinon residues in the fruits.

Keywords: Diazinon, Pesticides, Melon, Gaschromatograpy, Massspectrometry, SPE

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Type of Study: Research | Subject: General

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