Showing 8 results for Ovary
N Tawakal , A Mubarak Khalid , U Farooq Khan , K Raees Ahmad , Asmatullsh,
Volume 2, Issue 1 (3-2008)
Abstract
Introduction:Artifical sourace of radiation have increased worldwide average annual exposure of mankind and could especially affect rapidly dividing cells in gonads. Since, radiation sensitivities of different components of primordial follicles, and the time required for their deplation have not been morphologically eatablished we decided to estimate the time required for depletion of primordial follicles and to analyze the morphological changes in primordial follicles after gamma irradiation in rats ovaries.
Material & Method: 108-Female albino rats of 3 weeks age were divided into three groups. Out of them, 36 animals received 4.5 Gray, and 36 received 8.3 Gray of gamma radiation while rest received sham radiation. Then their Ovaries were dissected at 2hrs, 4hrs, 6hrs, one day, 7 days and 14 days after irradiation. Histological examination of five random sections of each ovary was performed.
Results: The stockpile of primordial follicles almost depleted within 24 hours. The pool of primordial follicles was greatly suppressed after irradiation showing primordial follicles radiosensitivity. Intense necrosis was evident with karyorrhexis and karyolysis in nucleus of oocyte while effects on granulosa cells were little and they increased the bulk of interstitial tissues.
Conclusion:Gamma ionizing radiation induces intense and rapid necrotic degeneration of oocyte in primordial ovarian follicles. The granulosa cells of degenerated primordial follicle increase the bulk of ovarian interstitial tissue. Resultant ovaries do not have enough follicular reserve for ovulatory process which may lead to early cessation of menstruation and eventually infertility.
Zinat Sargazi , Mohammad Reza Nikravesh, Mehdi Jalali , Hamid Reza Sadeghnia , Fatemeh Rahimi Anbarkeh, Leila Mohammadzadeh ,
Volume 8, Issue 26 (9-2014)
Abstract
Background: DZN (diazinon) is an organophosphate insecticide that had been used in agriculture and for domestic and veterinary use for several years and caused many negative effects on plants and animal species, especially on human. The aim of present study was to evaluate the effects of DZN on MDA (malondialdehyde) and GSH (glutathione) levels in female rat reproductive tissue (ovary) and to assess the protective role of vitamin E.
Methods: A total of 30 adult female Wistar rats were divided into five groups: control group (without any intervention), sham group (received only pure corn oil, as solvent), experimental group 1 (DZN+corn oil, 60 mg/kg), experimental group 2 (vitamin E, 200 mg/kg), and experimental group 3 (DZN+vitamin E, the same dosage). All drugs were injected intraperitoneally, except vitamin E which was administrated by gavage. The animals were scarified after two weeks and MDA as a marker of lipid peroxidation and GSH content were measured in ovarian tissue.
Results: DZN reduced GSH content and increased MDA level in ovary compared with the control group (P<0.001). Vitamin E plus DZN increased GSH content but decreased DZN-induced MDA elevation in rat ovarian tissue.
Conclusion: Oxidative stress contributes to DZN-induced ovarian toxicity. The results of this study suggested that vitamin E may have a protective effect on DZN-induced ovarian toxicity.
Nahid Rezaee, Zahra Nematollahi, Shahnaz Shekarforous, Ebrahim Hoseini,
Volume 10, Issue 2 (2-2016)
Abstract
Background: Many health problems are related to lifestyle and dietary factors. Since ancient times, food additives such as sulfites have been used to preserve foods. Diverse effects of sulfites on multiple organs have been reported but its effect on female reproductive organ has not been fully elucidated. The aim of this study was to investigate the effects of sodium metabisulfite (SMB) on ovarian tissue in adult rats.
Methods: Four groups of female rats (n=32) were used. The experimental rats received 10, 100 and 260 mg/kg SMB for 28 days (S10, S100 and S260 groups, respectively). The control rats received distilled water for the same period. The ovarian volume, weight and the number of different types of follicles were estimated by stereological methods. Lipid peroxidation is assessed indirectly by the measurement of malondialdehyde (MDA), using the thiobarbituric acid (TBA) method.
Results: The results showed a significant decrease in the ovarian volume, the number of primordial, primary, secondary, grafian follicles and corpus luteum in the SMB-treated animals compared with the control group (P < 0.05). In comparison to the control group, the number of atretic follicles increased in the SMB-treated rats. MDA was significantly increased in S260 group compared to the control group.
Conclusion: The present data confirm sulfite-induced structural changes in the ovary. Increased level of MDA because of SMB ingestion suggests that free radicals may have a critical role in these changes.
Zinat Sargazi, Mohammad Reza Nikravesh, Mehdi Jalali, Hamid Reza Sadeghnia, Fatemeh Rahimi Anbarkeh,
Volume 10, Issue 2 (2-2016)
Abstract
Background: Diazinon (DZN) is an organophosphate insecticide widely utilized in agriculture all over the world and causes many negative effects on plants and animal species, especially on human. The aim of present study was to evaluate the effects of DZN on apoptosis of ovarian follicles in adult rats and to assess the protective role of vit E.
Methods: Thirty adult female Wistar rats were divided into five groups: control group (without any intervention), sham group (received only pure olive oil, as solvent), experimental group 1 (DZN+olive oil, 60 mg/kg), experimental group 2 (vit E, 200 mg/kg), and experimental group 3 (DZN+vit E, the same dosage). All drugs were injected intraperitoneally, except vit E administrated by gavage. The animals were scarified after two weeks and left ovary was used to measure apoptosis of ovarian follicles.
Results: The number apoptotic cells experimental group 1 increased significantly in contrast control group in secondary and graffian follicles (P< 0.001). Administration vit E plus DZN, significantly reduced apoptotic cells compared to DZN group (P< 0.001).
Conclusion: DZN-induced apoptosis in secondary and graffian follicles and vit E inhibited apoptosis induced by DZN. Vit E might have a protective effect on DZN-induced ovarian toxicity.
Shahnaz Shekarforoush, Nahid Rezaee, Elaheh Farzanfar,
Volume 14, Issue 4 (10-2020)
Abstract
Background: Sulfites including Sodium Metabisulfite (SMB) are commonly used as food preservatives and pharmaceutical products. Despite their worldwide use, there is evidence suggesting their toxicity on human organs and tissues. The purpose of this study was to evaluate the effect of SMB with or without Zingiber officinale (ginger) extract on the rat ovary.
Methods: A total of 32 adult, female Wistar rats were divided into four groups of eight each. They consisted of, a) control group, b) ginger group (500 mg/kg/day), c) SMB group (260 mg/kg/day), and d) combined SMB and ginger group at identical doses. After 28 days, the rats were sacrificed and the ovarian tissue Malondialdehyde (MDA), as a marker of lipid peroxidation was measured. The volume and weight of the ovaries and the number of follicles at different stages were counted by stereological methods.
Results: The SMB treatment caused a significant decrease in the ovarian volume and the number of follicles with simultaneous increase in the number of degenerate follicles (P≤0.001) and MDA level (P≤0.01). Ginger treatment of the rats exposed to SMB significantly increased the number of follicles at various stages and partially reversed the ovarian tissue level of MDA, compared to that in the control group (P≤0.05).
Conclusion: The SMB treatment induced structural changes in the rats’ ovaries and the concomitant treatment with ginger largely reversed the damages caused by SMB.
Parastou Rad, Fahimeh Safari, Jamshid Mohammadi, Hamdollah Delaviz,
Volume 15, Issue 1 (1-2021)
Abstract
Background: Chemotherapy agents can cause ovarian dysfunction and eventually lead to infertility. This study investigated the effect of nasturtium officinale extract on the ovarian function following the toxicity induced by doxorubicin in female rats.
Methods: Forty eight female Wistar rats (180-210g) were randomly divided in six groups as follows: Group I, normal rats receiving 1ml normal saline; Group II and III receiving 25 and 75 mg/kg of the extract daily by gavage for 21 days. Groups IV, V and VI receiving 10 mg/kg doxorubicin intraperitoneally on the first day. In addition, Group IV and V received 25 and 75 mg/kg of the extract, respectively. The serum levels of estrogen, progesterone, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and ovarian Malondialdehyde (MDA) were determined after 21 days of treatment. The mean numbers of various graafian follicles and corpus lutea were recorded after treatment.
Results: The mean serum FSH level in Group VI (0.11±0.01) significantly reduced compared to those in Groups II (0.21±0.05) and III (0.23±0.01), (P<0.05). The mean serum LH and estrogen levels in Group VI (0.16±0.08) reduced insignificantly compared to those in the controls (0.21±0.02), and in Groups II (0.23±0.03) and III (0.22±0.09). A significant reduction in the number of primary, secondary and graafian follicles were observed in Group VI compared to the control group (P<0.05). The serum MDA level significantly declined in Group V compared to that in Group VI (P<0.05).
Conclusion: The nasturtium officinale extract attenuated the toxic effect of doxorubicin on the rat ovaries and protected the cell division in the follicles and the oocytes maturation.
Dr Nathaniel Amedu, Elizabeth Ajayi,
Volume 18, Issue 1 (1-2024)
Abstract
Background: Sodium metabisulfite (SMB) is a frequently utilized as food preservative. While it is generally acknowledged to be safe, there have been concerns regarding its potential impacts. This study aimed to investigate the effects of sodium metabisulfite on the hormonal levels, ovarian and uterine histology, and oxidative stress markers in female Wistar rats.
Methods: Twenty-four adolescent female Wistar rats were randomly allocated into four groups of siz rats each: Group 1 (control) received 0.5mL normal saline; Group 2 was given 100 mg/kg SMB; Group 3 received 300 mg/kg SMB; and Group 4 was administered 500 mg/kg SMB. The administration was done orally over 28 days, followed by euthanasia for tissue collection. Blood samples were collected to assess the serum follicle stimulating hormone (FSH) and luteinizing hormone (LH), while ovary and uterus tissue samples were harvested for malondialdehyde (MDA) assays and histopathology. For histopathology, we used haematoxylin and eosin and periodic acid schiff staining.
Results: The administration of SMB at doses of 300 and 500mg/kg had a notable impact on the hormone levels, particularly FSH and LH. The SMB doses also resulted in disrupted histo-architecture and altered glycogen expression in ovaries and uteri, as observed by histological examinations. Furthermore, SMB at 500mg/kg led to a significant increase in the oxidative stress marker malondialdehyde.
Conclusion: The SMB treatment affected FSH and LH levels, influencing ovarian and uterine structures. Disrupted structure and raised oxidative stress imply reproductive health risks. Further research is needed, including the effects of SMB on glycogen and FSH status.
Walaa A Kandeel, Seham A Farag, Hend M Ali, Mahmoud Ashry,
Volume 19, Issue 1 (2-2025)
Abstract
Abstract
One of the most prevalent metabolic conditions affecting women in their reproductive years that cause infertility or subfertility is Polycystic Ovary Syndrome, or PCOS. This was a pilot study to see whether Glycyrrhiza glabra L. aqueous extract (GAE) could prevent or treat PCOS in rats that had been given Estradiol®. Following acclimation, four groups of adult female Wistar albino rats weighing between 160 and 200 grams each were randomly assigned; Normal rats were used in the first group as a control, In the second group, normal rats were given 100 mg/kg of Glycyrrhiza glabra L orally for a period of six weeks, Third group: animals modelled by Polycystic Ovary Syndrome that were not treated served as a positive control, and lastly, Glycyrrhiza glabra L was administered to a group of animals modeled for PCOS for a period of six weeks. The obtained results showed that the PCOS caused by Estradiol® was significantly restored by Glycyrrhiza glabra L extracts; The results demonstrated a considerable reduction in serum levels of FSH, LH, PRL, testosterone, E2, TNF-α, IL-1β, IL-4, IL-6, and IL-10, along with a slight increase in insulin and progesterone levels, indicating that Glycyrrhiza glabra L extract significantly controlled PCOS deteriorations. Comparably, a noteworthy improvement in the oxidative status was noted; ovarian SOD, GPx, CAT, and GSH levels were significantly raised, accompanied by a notable decrease in MDA and NO levels. Additionally, urea, creatinine, cholesterol, triglycerides, and ALAT and ASAT activities were significantly reduced, and GAE showed nephro-hepatoprotective and anti-atherogenic capabilities; these results were in line with the comparable values of the healthy group. Histological analysis corroborated these results. In summary, GAE's phytochemicals, antioxidant, and anti-inflammatory qualities, along with its capacity to cause folliculogenesis, allowed it to effectively alleviate the effects of Estradiol®-induced polycystic ovary.
