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Showing 2 results for Phycocyanin
Apoptotic Effect of Phycocyanin on HT-29 Colon Cancer through Activation of Caspase Enzymes and P53 Cell Signaling Pathway
Mr. Abbas Asoudeh-Fard, Dr. Reza Najafipour, Mrs. Mitra Salehi, Mrs. Mahsa Mahmoudi, Dr. Iman Salahshourifar, Dr. Anoosh Eghdami, Dr. Asghar Parsaei, Dr. Hossein Piri,
Volume 18, Issue 1 (1-2024)
Abstract
Background: C-phycocyanin, a biliprotein from Spirulina platensis, is a future candidate for cancer management. This agent is originated from edible blue-green algae, and numerous in vivo and in vitro research have reported on its anti-cancer properties. The effects of C-phycocyanin have been investigated on caspases 3, 8, 9, and p53 pathways in the human colorectal adenocarcinoma cell line (HT-29) and human umbilical vein endothelial cells (HUVECs).
Methods: In the current study, we investigated the effect of C-phycocyanin on caspase 3, 8, 9, and p53-mediated apoptosis pathways in two cell lines (HT-29 & HUVEC), using quantitative real-time PCR and flow cytometry. The cytotoxicity of phycocyanin on HT-29 cells was compared with HUVEC normal cells via colorimetric assays.
Results: Based on our findings at molecular level, the expression of caspases 3, 8, 9, and p53 genes were increased in colorectal cancer cells treated with C-phycocyanin.The results were confirmed by an increase in the number of colorectal cancer cells in the early and late stages of apoptosis as compared to the control, untreated cells. In addition, the results of colorimetric assay showed that C-phycocyanin has no cytotoxic effects on normal HUVECs cells.
Conclusion: Based on our experimental data, it is evident that C-phycocyanin has measurable effects on cell apoptosis. Since tumorigenesis is halted by apoptosis, C-phycocyanin can be a hopeful candidate for the treatment of human colorectal cancer in the future.

Novel Effects of Phycocyanin Extract on the Regulation of Apoptosis Genes in Human Colorectal Cancer Cells
Mr. Abbas Asoudeh-Fard, Dr. Reza Najafipour, Mr. Amir Hadi Hosseini, Mrs. Faezeh Razi, Dr. Iman Salahshourifar, Dr. Mohammad Zareian Jahromi, Dr. Asghar Parsaei, Dr. Hossein Piri,
Volume 18, Issue 2 (8-2024)
Abstract
Background: Cancer is a major cause of death worldwide, second only to cardiovascular disease. Many factors influence the development of cancer, including environmental, genetic, and lifestyle factors. Although the prognosis of colon cancer treatment has improved significantly in recent years, there is still much clinical need to explore new treatment strategies with anticancer and immune-enhancing effects. Phycocyanin (PC) is an important compound isolated from blue-green algae and a well-known antioxidant that suppresses oxidative reactions in cells. It also has other useful functions, such as high efficiency, low toxicity, and antitumor properties. We investigated the potential antiapoptotic effect of PC in vitro on human normal and cancer cell lines.
Methods: Phycocyanin extracted from Streptomyces platensis algae has the potential to become a turning point in pharmaceutical research. In this study, PC was used as a natural anticancer agent and apoptosis activator to increase the expression of the PTEN and Bax genes in HT-29 cancer and normal cells (HUVEC). The effect of treatment with PC was evaluated using an MTT assay and flow cytometry.
Results: The results showed that PC can have apoptotic effects on the human colon cancer cell line (HT-29) by up-regulating PTEN and Bax genes and down-regulating AKT and BCL-2 genes.
Conclusion: Based on the findings, PC can be considered a herbal treatment and a useful supplement in the management of human colorectal cancer.

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