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1- Biology Education, Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Sukabumi, Sukabumi, Indonesia , ginanuranti@ummi.ac.id
2- Biology Education, Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Sukabumi, Sukabumi, Indonesia
2- Biology Education, Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Sukabumi, Sukabumi, Indonesia
Abstract: (9 Views)
Background: Karas Tulang (Chloranthus erectus), a plant from the Chloranthaceae family, has been traditionally used as tea in Indonesia for its potential to enhance stamina and prevent fatigue. Despite its various biological and pharmacological properties, including antioxidant, antibacterial, and anti-inflammatory activities, the safety and toxicity of C. erectus have not been extensively studied. This study aimed to assess the acute toxicity of the methanol extract of C. erectus leaves on mice (Mus musculus) using the OECD 420 method.
Methods: Acute toxicity testing was conducted by administering a single dose of the methanol extract to female Swiss Webster mice at varying doses (5, 50, 300, and 2000 mg/kg BW). Throughout the 14-day observation period, no significant signs of toxicity or mortality were observed, even at the highest dose (2000 mg/kg).
Results: Statistical analysis using one-way ANOVA and post-hoc tests revealed no significant differences in the organ weight index of mice across all treatment groups (p > 0.05). Based on an estimated LD50 value greater than 2000 mg/kg, the methanol extract of C. erectus can be classified as a Category 5 compound with low toxicity, according to the Globally Harmonized System (GHS) classification.
Conclusions: These findings suggest that the methanol extract of C. erectus leaves has a relatively safe toxicity profile, consistent with its traditional use as an herbal remedy. However, further studies are required to evaluate the long-term safety and potential therapeutic applications of this plant.
Methods: Acute toxicity testing was conducted by administering a single dose of the methanol extract to female Swiss Webster mice at varying doses (5, 50, 300, and 2000 mg/kg BW). Throughout the 14-day observation period, no significant signs of toxicity or mortality were observed, even at the highest dose (2000 mg/kg).
Results: Statistical analysis using one-way ANOVA and post-hoc tests revealed no significant differences in the organ weight index of mice across all treatment groups (p > 0.05). Based on an estimated LD50 value greater than 2000 mg/kg, the methanol extract of C. erectus can be classified as a Category 5 compound with low toxicity, according to the Globally Harmonized System (GHS) classification.
Conclusions: These findings suggest that the methanol extract of C. erectus leaves has a relatively safe toxicity profile, consistent with its traditional use as an herbal remedy. However, further studies are required to evaluate the long-term safety and potential therapeutic applications of this plant.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
