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Volume 20, Issue 3 (July 2026)
IJT 2026, 20(3): 0-0 |
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Ethics code: IR.ARUMS.AEC.1401.046
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Mahdavifard S, Irani S. The Hepato-Cardioprotective Effect of Thiamine in a CCl4-Induced Oxidative Stress Rat Model by Enhancing Hepatic and Cardiac Antioxidant Potential. IJT 2026; 20 (3)
URL: http://ijt.arakmu.ac.ir/article-1-1597-en.html
URL: http://ijt.arakmu.ac.ir/article-1-1597-en.html
1- Department of Clinical Biochemistry, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. & Non-Communicable Diseases Research Center, Research Institute for Prevention of Non-communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran. , s.mahdavifard@qums.ac.ir
2- Department of Clinical Biochemistry, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
2- Department of Clinical Biochemistry, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Abstract: (27 Views)
Background: Thiamine deficiency heightens tissue sensitivity to toxins by increasing oxidative stress and promoting inflammation. Various toxins deplete glutathione (GSH) levels, which are essential for cellular signaling, particularly within the NF-κB pathway that regulates responses to harmful substances. Maintaining balance in the NF-κB pathway and GSH is vital for toxin protection. We examined rat liver and heart tissues exposed to carbon tetrachloride (CCl4).
Methods: A total of 40 rats were allocated into four experimental groups: group 1 (control), group 2 (control supplemented with thiamine), group 3 (OSR-untreated), and group 4 (OSR supplemented with thiamine). The rats in the thiamine-treated groups were given 180 mg/L daily for a period of two weeks through their drinking water. Oxidative stress was induced by injecting 1 ml/kg of CCl4 intraperitoneally. Levels of GSH, GSSG, and NF-κB expression were assessed, along with indicators of liver and heart function. Liver tissue was analyzed under a microscope by a pathologist.
Results: Thiamine improved hepatic and cardiac function, reduced inflammation, and enhanced the antioxidant capacity of the body, liver, and heart against oxidative stress. Additionally, it downregulated NF-κB activity in both the liver and heart and prevented hepatic damage and fat accumulation, with effects reaching statistical significance (p<0.001).
Conclusion: Thiamine protected the liver and heart against oxidative stress-induced damage by enhancing antioxidant capacity and reducing NF-κB signaling and inflammation. This protection was achieved through boosting the body's ability to fight oxidative stress and lowering biomolecule oxidation. Downregulation of NF-κB levels and enhancement of GSH metabolism appears to be crucial ways in which thiamine safeguards both the liver and the heart.
Methods: A total of 40 rats were allocated into four experimental groups: group 1 (control), group 2 (control supplemented with thiamine), group 3 (OSR-untreated), and group 4 (OSR supplemented with thiamine). The rats in the thiamine-treated groups were given 180 mg/L daily for a period of two weeks through their drinking water. Oxidative stress was induced by injecting 1 ml/kg of CCl4 intraperitoneally. Levels of GSH, GSSG, and NF-κB expression were assessed, along with indicators of liver and heart function. Liver tissue was analyzed under a microscope by a pathologist.
Results: Thiamine improved hepatic and cardiac function, reduced inflammation, and enhanced the antioxidant capacity of the body, liver, and heart against oxidative stress. Additionally, it downregulated NF-κB activity in both the liver and heart and prevented hepatic damage and fat accumulation, with effects reaching statistical significance (p<0.001).
Conclusion: Thiamine protected the liver and heart against oxidative stress-induced damage by enhancing antioxidant capacity and reducing NF-κB signaling and inflammation. This protection was achieved through boosting the body's ability to fight oxidative stress and lowering biomolecule oxidation. Downregulation of NF-κB levels and enhancement of GSH metabolism appears to be crucial ways in which thiamine safeguards both the liver and the heart.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
