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Volume 7, Issue 20 (Spring 2013)
IJT 2013, 7(20): 808-815 |
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Karami M, Saeidnia S, Naghshvar F. The Hepatoprotective Effects of Corn Silk against Dose-induced Injury of Ecstasy (MDMA) Using Isolated Rat Liver Perfusion System. IJT 2013; 7 (20) :808-815
URL: http://ijt.arakmu.ac.ir/article-1-195-en.html
URL: http://ijt.arakmu.ac.ir/article-1-195-en.html
1- Department of Toxicopharmacology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran , toxkarami@gmali.com
2- Medical Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Pathology, School of Medical Sciences, Mazandaran University of Medical Sciences, Sari, Iran
2- Medical Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Pathology, School of Medical Sciences, Mazandaran University of Medical Sciences, Sari, Iran
Abstract: (10277 Views)
Background: Corn silk (CS) is widely used in Iranian traditional medicine. The aim of this study was to investigate hepatoprotective activity of CS by Isolated Rat Liver Perfusion System (IRLP).
Methods: Hydro-alcoholic extract of corn silk (10, 20, 40, and 100 mg kg-1) was evaluated for its hepatoprotective activity by IRLP. Phenol and flavonoid contents of the extract were determined as gallic acid and quercetin equivalents from a calibration curve, respectively. IRLP system is ideal for studying biochemical alterations of chemicals with minimum neuro-hormonal effects. In this study, the liver was perfused with Kerbs-Henseleit buffer, containing different concentration of hydro-alcoholic extract of corn silk (10, 20, 40, 50,100mg/kg), added to the buffer, and perfused for 2 hours. During the perfusion, many factors, including amino-transferees activities and the level of GSH, were assessed as indicators of liver viability. Consequently, sections of liver tissues were examined for any histopathological changes.
Results: Histopathological changes in liver tissues were related to hydro-alcoholic extract of corn silk concentrations in a dose-dependent manner. Also, 50 and 100mg/kg doses caused significant (P<0.05) histopathological changes. Level of GSH in samples perfused with hydro-alcoholic extract increased compared to the control group.
Conclusion: Hepatoprotective effect of CS is due to decreased lipid peroxidation, although other mechanisms might also be involved.
Methods: Hydro-alcoholic extract of corn silk (10, 20, 40, and 100 mg kg-1) was evaluated for its hepatoprotective activity by IRLP. Phenol and flavonoid contents of the extract were determined as gallic acid and quercetin equivalents from a calibration curve, respectively. IRLP system is ideal for studying biochemical alterations of chemicals with minimum neuro-hormonal effects. In this study, the liver was perfused with Kerbs-Henseleit buffer, containing different concentration of hydro-alcoholic extract of corn silk (10, 20, 40, 50,100mg/kg), added to the buffer, and perfused for 2 hours. During the perfusion, many factors, including amino-transferees activities and the level of GSH, were assessed as indicators of liver viability. Consequently, sections of liver tissues were examined for any histopathological changes.
Results: Histopathological changes in liver tissues were related to hydro-alcoholic extract of corn silk concentrations in a dose-dependent manner. Also, 50 and 100mg/kg doses caused significant (P<0.05) histopathological changes. Level of GSH in samples perfused with hydro-alcoholic extract increased compared to the control group.
Conclusion: Hepatoprotective effect of CS is due to decreased lipid peroxidation, although other mechanisms might also be involved.
Keywords: Glutathione, Krebs-Henseleit, Liver Injury, N-Methyl-3, Zea Mays, 4-Methylenedioxyamphetamine.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
