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:: Volume 10, Issue 1 (January-February 2016) ::
IJT 2016, 10(1): 31-40 Back to browse issues page
Vitamin C and Chitosan Alleviate Toxic Effects of Paraquat on Some Biochemical Parameters in Hepatocytes of Common Carp
Zeinab Sharifinasab , Mahdi Banaee , Mohammad Mohiseni , Ahmad Noori
Department of Aquaculture, Behbahan Khatam Alanbia University of Technology, Iran. , banaee@bkatu.ac.ir
Abstract:   (2333 Views)

Background: Paraquat is nonselective bipyridyl herbicide that induces hepatotoxicity through oxidative stress. Vitamin C and chitosan have antioxidant as well as radical scavenger properties and show protective effects against reactive oxygen species (ROS). In the present study, hepatoprotective effects of chitosan and vitamin C were evaluated in common carp exposed to paraquat.

Methods: While exposed to 0.02 mg. L-1paraquat for 21 days, common carp were fed chitosan (1000 mg. kg‑1 feed), vitamin C (1000 mg. kg-1 feed), and vitamin C combined with chitosan. At the end of the experiment, activities of hepatic enzymes and oxidative stress biomarkers were evaluated.

Results: Paraquat induces changes in the activity of alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, alkaline phosphatase and lactate dehydrogenase in liver tissue of fish. However, these enzymes were restored to normal levels in fish fed with vitamin C and vitamin C combined with chitosan following exposure to paraquat. Increased levels of malondialdehyde were observed in liver after exposure to paraquat, while glucose-6-phosphate dehydrogenase and catalase activities and the total antioxidant levels decreased. Administration of vitamin C combined with chitosan significantly reduced malondialdehyde levels and increased the total antioxidant capacity, glucose-6-phosphate dehydrogenase and catalase activities.

Conclusion: Administration of vitamin C is effective in reducing liver toxicity of paraquat. However, administrating both vitamin C and chitosan is more effective. In other words, chitosan and vitamin C have a synergic effect. They could be used as hepatoprotective agents against paraquat-induced hepatotoxicity in fish.

Keywords: Biochemical parameters, Chitosan, Oxidative stress, Paraquat, Vitamin C.
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Type of Study: Research | Subject: Special
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Sharifinasab Z, Banaee M, Mohiseni M, Noori A. Vitamin C and Chitosan Alleviate Toxic Effects of Paraquat on Some Biochemical Parameters in Hepatocytes of Common Carp. IJT. 2016; 10 (1) :31-40
URL: http://ijt.arakmu.ac.ir/article-1-430-en.html

Volume 10, Issue 1 (January-February 2016) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
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