[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 10, Issue 3 (May-June 2016) ::
IJT 2016, 10(3): 13-17 Back to browse issues page
Effects of Beta-Naphthoflavone and 3-Methylcholantheren on Biochemical Markers in Sturgeon Fish, Huso huso
Katayoon Karimzadeh
Department of Marine Biology, Islamic Azad University, Branch of Lahijan 1616, Lahijan, Iran. , karimzadehkatayoon@liau.ac.ir
Abstract:   (1613 Views)

Background: Polycyclic aromatic hydrocarbons (PAHs) are a predominant group of contaminants that have been shown to accumulate and persist in marine organisms. We evaluated the dose-dependent induction of the cytochrome P4501A (CYP1A) system in the Sturgeon fish, Huso huso after after exposure to PAHs, beta-naphthoflavone (BNF) and 3- methylcholantheren.

Methods: The fish were treated by i.p. injections of beta–naphthoflavone and 3- methylcholantheren dissolved in corn oil at three doses 35, 70 and 105 mg/kg wet-body weight respectively for 72 h every day. Similarly, the control fish received injections of only corn oil. Microsomal fraction was prepared and ethoxy resorufin-O-deethylase activity (EROD) was measured in the fish liver. Cytochrome P4501A1 (CYP1A1) content was estimated by ELISA based on monoclonal anti-cod P4501A1.

Results: Beta-naphthoflovone and 3-methylcholantheren treated-fish developed 15-32 fold increase in Ethoxyresorufin-O-deethylase activity in liver microsomes. The results of ELISA showed higher levels of cytochrome P4501A content in treated fish as compared to controls.

Conclusion: A parallel increase of the CYP1A immunochemically assay and an increase in EROD activity could be recorded for beta –naphthoflavone and 3- methylcholantheren in injected fish. Dose-response relationships on the induction of EROD and CYP1A immunodetectable protein were recorded. Therefore, EROD activity and CYP1A content can be used as a biomarker of polycyclic aromatic hydrocarbons.

Keywords: 3-Methylcolantheren, Beta–Naphthoflavon, Biomarker, Ethoxyresorufin Deethylase, Huso Huso.
Full-Text [PDF 150 kb]   (687 Downloads)    
Type of Study: Research | Subject: General
References
1. Hassanain MA, Abdel-Rahman E H, Abo-Hegab S, Tawfik MA,Abbas W T. Induction of cytochrome P450 1A1 as a biomarker of Benzo-a-pyrene pollution in Egyptian freshwater fish. Pak J Bio Sci 2007, 10(8):1161-69. [DOI:10.3923/pjbs.2007.1161.1169]
2. Barra R, Sanchez-Hernandez J C, Orrego R, Parra O, Gavilan J F.Bioavailability of PAHs in the Biobio river (Chile): MFO activity and biliary fluorescence in juvenile Oncorhynchus mykiss. Chemosphere 2001; 45: 439-44. [DOI:10.1016/S0045-6535(01)00041-8]
3. Arinc E, Sen A. Effects of in vivo benzo (a) pyrene treatment on liver. Mixed function oxidase activities of gilthead seabream (Sparusaurata). Comp Biochem Physiol Pharmacol Toxicol Endocrinol 1994; 107C: 405-14. [DOI:10.1016/1367-8280(94)90069-8]
4. Emborski C, Reyes A , Biggs J S. Effect of b-naphthoflavone on hepatic cytochrome P4501A activity in the scribbled rabbitfish (Siganusspinus) from tropical Indo-Pacific coral reefs. Ecotoxicol 2012; 21:2153–62. [DOI:10.1007/s10646-012-0969-1]
5. Almeida L, Froneman W, plestschke B. Optimization of a Cytochrome-P450-Monooxygenase-1A-Mediated EROD Assay in the Cape Hake Species Merluccius capensis and Merluccius paradoxus (Pisces). Enzyme Res 2011; 108395:1-8. [DOI:10.4061/2011/108395]
6. Kautz C, Reyes A, Biggs J. Effect of β-napthoflavone on hepatic cytochrome P4501A activity in the scribbled rabbitfish (Siganus spinus) from tropical Indo-Pacific coral reefs. Ecotoxicol 2012; 21 (8):2153-62. [DOI:10.1007/s10646-012-0969-1]
7. Coimbra Rola R, Costa Monteiro M, Renan da Silva Reis S R, Zomer Sandrini J. Molecular and biochemical biomarkers responses in the mussel Mytilus edulis collected from Southern Brazil coast. Mar Poll Bulletin 2012; 64:766-77. [DOI:10.1016/j.marpolbul.2012.01.004]
8. Cajaraville M P, Bebianno M J, Blasco J, Porte C, Sarasquete C, Viarengo A. The use of biomarkers to assess the impact of pollution in coastal environments of the Iberian Peninsula: a practical approach. Sci Total Environ 2000; 247: 295–311. [DOI:10.1016/S0048-9697(99)00499-4]
9. Sirosoka Z, Drastichova J. Biochemical markers of aquatic environment contamination, cytochrome P450 in Fish. A review. Acta Vet Brno 2004; 73: 123-32.
10. Cao X, Song Y, Shihua F, Kai j, Yang X, Chen L. Optimization of Ethoxyresorufin-O-deethylase Determination in the Microsomes of Earthworms and Its Induction by Polycyclic Aromatic Hydrocarbons. Clean – Soil, Air, Water 2013; 41 (9999): 1–5.
11. Addison RF. The use of biological effects monitoring in studies of marine pollution. Environ Rev.1996; 4: 225-37. [DOI:10.1139/a96-012]
12. Yi B, Yange J, Yang M. Past and future applications of CYP450-genetic polymorphisms for bio monitoring of environmental toxicants. J Environ Sci Health 2007; 25:353–77. [DOI:10.1080/10590500701704037]
13. Oritzdemontellano PR. Structure, mechanism and inhibition of cytochrome P450. Drug Metab Dispos 1995; 23:1181-87.
14. Schollz S, .Behan I, Honec T, Hauc C, Braunbeck T, Segner H. Development of a monoclonal antibody for ELISA of CYP1Ain primary culture of rainbow trout (Oncorhynchus mykiss) hepatocytes. Biomarkers 1997; 287-94. [DOI:10.1080/135475097231517]
15. Karimzadeh K, Zahmatkesh A. biomarker responses in Persian Sturgeon, Acipenser persicus exposed to benzo -apyrene and beta-naphtoflavon. Arch Biol Sci Belgrade 2013; 65 (4), 1397-1403. [DOI:10.2298/ABS1304397K]
16. Kajiwara N, Ueno D, Monirith I, Tanabe S, Pourkazemi M, Aubrey D G. Contamination by organochlorine compounds in sturgeons from Caspian Sea during 2001-2002. Mar Poll Bulletin 2003; 46: 741-47. [DOI:10.1016/S0025-326X(03)00047-X]
17. Sarasquete C, Senger H. Cytochrome P4501A in teleostean Fishes. The Sci total Environ 2001; 247: 313-32. [DOI:10.1016/S0048-9697(99)00500-8]
18. Nilsen B, Berg K, Goksøyr A. Induction of cytochrome P450 1A (CYP1A) in fish. Method Mole Biol 1998; 107:423–38.
19. Lowry OH, Rosebrough NJ Farr A I. Randall RJ. Protein measurement with the Folin phenol reagent. J Bio Chem 1951; 193:265-75.
20. Burke MD, Mayer, RT. Direct flurometric assay of microsomal O-dealkylation which preferentially induced by 3-methylcholantrene. Drug Metab Dispos 1974; 2: 583-88.
21. Karimzadeh K, Mostafaei M, Esmaili S A, PourkazemiMand A. Zahmatkesh A. Induction and purification of Cytochrome P4501A1 from ß-naphthoflavon- treated Beluga, Huso huso. J App Ichthyol 2006; 22: 221-25. [DOI:10.1111/j.1439-0426.2007.00955.x]
22. Goksøyr A, Forlin L. The cytochrome P450 system in fish, aquatic toxicology and environmental monitoring. Aquat Toxicol 1992; 22:287–312 [DOI:10.1016/0166-445X(92)90046-P]
23. Morinmet D,Taysse B, Deschaux P. 3-Methylcholantheren-induced EROD activity in carp (Cyprinus carpio) .Comp Biochem Biophysiol 1999; 124(2): 165-70.
24. Stegeman JJ,Woodin B R, Singh H, Oleksiak M F,Celander M. Cytochrome P450 (CYP) in tropical fishes: Catalytic Activities, Expression of multiple CYP proteins and high levels microsomalP450 in liver of fishes from Bermoda. Com Biochem Physiol1997; 116(C): 61-75. [DOI:10.1016/S0742-8413(96)00128-4]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

karimzadeh K. Effects of Beta-Naphthoflavone and 3-Methylcholantheren on Biochemical Markers in Sturgeon Fish, Huso huso. IJT. 2016; 10 (3) :13-17
URL: http://ijt.arakmu.ac.ir/article-1-436-en.html


Volume 10, Issue 3 (May-June 2016) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3781