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:: Volume 10, Issue 5 (September-October 2016) ::
IJT 2016, 10(5): 7-10 Back to browse issues page
Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran
Somayyeh Gharibi , Iraj Nabipour , Euikyung Kim , Seyede Maryam Ghafari , Seyed Mehdi Hoseiny , Mostafa Kamyab , Ramin Seyedian *
Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran. , raminseyedian@gmail.com
Abstract:   (1113 Views)

Background: Cutaneous reactions like pruritus and erythema are common in warm months of the year in Bushehr Port, Persian Gulf, Iran due to jellyfish envenomation. This study reports isolation of the Chrysaora hysoscella nematocysts and evaluating its pharmacological activities during a bloom in 2013.

Methods: The venom of C. hysoscella captured in Jofre area in Bushehr port was analyzed. The electrophoretic profile was assessed by SDS-PAGE (12.5%) and the crude sample was analyzed using reverse phase HPLC. Caseinase activity was also determined.

Results: After separation of tentacles and isolation of their nematocysts, three different major protein components were revealed at 72-250 kDa with SDS-PAGE, signifying the presence of peptides in its venom. Two major peaks at 8.62 and 11.23 min were observed in reverse phase HPLC of the crude venom denoting protease peptide structural identities. Caseinase activity of C. hysoscella's venom was extremely low as compared with other jellyfish venoms.

Conclusion: This was the first report on the structural examination of jellyfish in Persian Gulf and may pave the way for determination and separation of destructive enzymes inducing cutaneous reactions in fishermen and swimmers.

Keywords: Caseinase, Cnidarian Venoms, Nematocyst, Scyphozoa, Sea Nettle.
Full-Text [PDF 262 kb]   (716 Downloads)    
Type of Study: Research | Subject: General
References
1. Lynam CP, Gibbons MJ, Axelsen BE, Sparks CA, Coetzee J, Heywood BG, et al. Jellyfish overtake fish in a heavily fished ecosystem. Curr Biol 2006;16(13):R492-R3. [DOI:10.1016/j.cub.2006.06.018]
2. Pauly D, Christensen V, Guénette S, Pitcher TJ, Sumaila UR, Walters CJ, et al. Towards sustainability in world fisheries. Nature 2002;418(6898):689-95. [DOI:10.1038/nature01017]
3. Kogovšek T, Bogunović B, Malej A. Recurrence of bloom-forming scyphomedusae: wavelet analysis of a 200-year time series. Hydrobiologia 2010;645(1):81-96. [DOI:10.1007/s10750-010-0217-8]
4. Daryanabard R, Dawson MN. Jellyfish blooms: Crambionella orsini (Scyphozoa: Rhizostomeae) in the Gulf of Oman, Iran, 2002–2003. JMBA 2008;88(03):477-83. [DOI:10.1017/S0025315408000945]
5. Bloom DA, Burnett JW, Alderslade P. Partial purification of box jellyfish (Chironex fleckeri) nematocyst venom isolated at the beachside. Toxicon 1998;36(8):1075-85. [DOI:10.1016/S0041-0101(98)00096-8]
6. Ramasamy S, Isbister GK, Seymour JE, Hodgson WC. Pharmacologically distinct cardiovascular effects of box jellyfish (Chironexfleckeri) venom and a tentacle-only extract in rats. Toxicol Lett 2005;155(2):219-26. [DOI:10.1016/j.toxlet.2004.09.018]
7. Kang C, Munawir A, Cha M, Sohn E-T, Lee H, Kim J-S, et al. Cytotoxicity and hemolytic activity of jellyfish Nemopilema nomurai (Scyphozoa: Rhizostomeae) venom. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 2009;150(1):85-90. [DOI:10.1016/j.cbpc.2009.03.003]
8. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72(1-2):248-54. [DOI:10.1016/0003-2697(76)90527-3]
9. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5. [DOI:10.1038/227680a0]
10. Ghafari SM, Jamili S, Bagheri KP, Ardakani EM, Fatemi MR, Shahbazzadeh F, et al. The first report on some toxic effects of green scat, Scatophagus argus an Iranian Persian Gulf venomous fish. Toxicon 2013;66:82-7. [DOI:10.1016/j.toxicon.2013.01.025]
11. Purcell JE, Uye S-i, Lo W-T. Anthropogenic causes of jellyfish blooms and their direct consequences for humans: a review. Mar Ecol Prog Ser 2007;350:153-74. [DOI:10.3354/meps07093]
12. Parodi A, Bassi A, Catalano M, Giacco E, Mariottini G, Pane L. Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria. Boll Soc Ital Biol Sper 2009;82(1):1-2. [DOI:10.4081/4734]
13. Kokelj F, Stinco G, Del Negro P. [Dermatotoxicity of the Adriatic jellyfish]. G Ital Dermatol Venereol 1990;125(12):575-7.
14. Veraldi S, Carrera C. Delayed cutaneous reaction to jellyfish. Int J Dermatol 2000;39(1):28-9. [DOI:10.1046/j.1365-4362.2000.00823.x]
15. Al-Rubiay K, Al-Musaoi H, Alrubaiy L, Al-Freje M. Skin and systemic manifestations of jellyfish stings in Iraqi fishermen. Libyan J Med 2009;4(2):1-2.
16. Radwan FF, Burnett JW, Bloom DA, Coliano T, Eldefrawi ME, Erderly H, et al. A comparison of the toxinological characteristics of two Cassiopea and Aurelia species. Toxicon 2001;39(2):245-57. [DOI:10.1016/S0041-0101(00)00121-5]
17. Radwan FF, Gershwin L-A, Burnett JW. Toxinological studies on the nematocyst venom of Chrysaora achlyos. Toxicon 2000;38(11):1581-91. [DOI:10.1016/S0041-0101(00)00092-1]
18. Burnett JW, Calton GJ, Burnett HW. Jellyfish envenomation syndromes. J Am Acad Dermatol 1986;14(1):100-6. [DOI:10.1016/S0190-9622(86)70013-3]
19. Nevalainen TJ, Peuravuori HJ, Quinn RJ, Llewellyn LE, Benzie JA, Fenner PJ, et al. Phospholipase A2 in cnidaria. Comp Biochem Physiol B Biochem Mol Biol 2004;139(4):731-5. [DOI:10.1016/j.cbpc.2004.09.006]
20. Lee H, Jung E-s, Kang C, Yoon WD, Kim J-S, Kim E. Scyphozoan jellyfish venom metalloproteinases and their role in the cytotoxicity. Toxicon 2011;58(3):277-84. [DOI:10.1016/j.toxicon.2011.06.007]
21. Sparks C, Buecher E, Brierley AS, Axelsen BE, Boyer H, Gibbons MJ. Observations on the distribution and relative abundance of the scyphomedusan Chrysaora hysoscella (Linné, 1766) and the hydrozoan Aequorea aequorea (Forskål,1775) in the northern Benguela ecosystem. Jellyfish Blooms: Ecological and Societal Importance: Springer; 2001. p. 275-86. [DOI:10.1007/978-94-010-0722-1_22]
22. Flynn B, Gibbons M. A note on the diet and feeding of Chrysaora hysoscella in Walvis Bay Lagoon, Namibia, during September 2003. Afr J Mar Sci 2007;29(2):303-7. [DOI:10.2989/AJMS.2007.29.2.15.197]
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Gharibi S, Nabipour I, Kim E, Ghafari S M, Hoseiny S M, Kamyab M et al . Characterization and Pharmacological Activities of Jellyfish, Chrysaora hysoscella Captured in Bushehr Port, Iran. IJT. 2016; 10 (5) :7-10
URL: http://ijt.arakmu.ac.ir/article-1-508-en.html


Volume 10, Issue 5 (September-October 2016) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
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