Write your message
Volume 11, Issue 1 (January-Fabruary 2017)                   IJT 2017, 11(1): 55-58 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sulyman A O, Akolade J O, Na’Allah A, Aladodo R A, Jamiu H O. Effect of Administration of Root Ethanolic Extract of Aristolochia Ringens on the Liver Functional Indices of Male Wistar Rats. IJT. 2017; 11 (1) :55-58
URL: http://ijt.arakmu.ac.ir/article-1-535-en.html
1- Department of Biosciences and Biotechnology (Biochemistry Unit), Kwara State University, Malete, Ilorin, Nigeria. , abdulhakeem.sulyman@kwasu.edu.ng
2- MSc of Biochemistry, Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex, Sheda, Abuja, Nigeria.
3- Department of Biological Sciences (Biochemistry Unit) Al-Hikmah University, Ilorin, Nigeria.
4- Department of Biosciences and Biotechnology (Biochemistry Unit), Kwara State University, Malete, Ilorin, Nigeria.
Abstract:   (3520 Views)

Background: The alcoholic decoction of root ethanolic extract of Aristolochia ringens is taken orally to treat various ailments in South-west Nigeria without prior knowledge of its potential toxic effect. Therefore, this study aimed at assessing the toxicity potentials of root ethanolic extract of A. ringens on functional indices and histology of the liver.

Methods: Twenty male rats were randomized into four groups of five animals each. Group A (control) received 0.5 ml of distilled water, group B, C and D received 75, 150 and 300 mg/kg b. wt. of the extract respectively. The administration was done orally and lasted for fourteen days.

Results: The extract significantly reduced the activities of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT) as well as reduction in the level of serum albumin and direct bilirubin (P<0.05) while the level of total bilirubin increased. The activities of these enzymes i.e. ALP, ALT and AST increased in the serum at all the doses investigated.

Conclusion: Ethanolic extract from A. ringens root may not be completely safe when administered repeatedly.

Full-Text [PDF 125 kb]   (911 Downloads)    
Type of Study: Research | Subject: General

1. Da Costa Lopes L, Albano F, Laranja GAT, Alves LM, e Silva LFM, de Souza GP, et al. Toxicological evaluation by in vitro and in vivo assays of an aqueous extract prepared from Echinodorus macrophyllus leaves. Toxicol Lett 2000;116(3):189-98. [DOI:10.1016/S0378-4274(00)00220-4]
2. Balick MJ, Cox PA. Plants, people, and culture: the science of ethnobotany: Scientific American Library; 1996.
3. Adeyemi O, Aigbe F, Badru O. The antidiarrhoeal activity of the aqueous root extract of Aristolochia ringens (Vahl.) Aristolochiaceae. Nig Qt J Hosp Med 2012;22(1):29-33.
4. Sonibare M, Gbile Z. Ethnobotanical survey of anti-asthmatic plants in South Western Nigeria. Afr J Tradit Complementary Altern Med 2008;5(4):340-5. [DOI:10.4314/ajtcam.v5i4.31288]
5. Akindele AJ, Wani Z, Mahajan G, Sharma S, Aigbe FR, Satti N, et al. Anticancer activity of Aristolochia ringens Vahl.(Aristolochiaceae). J Tradit Complemen Med 2015;5(1):35-41. [DOI:10.1016/j.jtcme.2014.05.001]
6. Sulyman AO, Akolade JO, Sabiu S, Aladodo RA, Muritala HF. Antidiabetic potentials of ethanolic extract of Aristolochia ringens (Vahl.) roots. J Ethnopharmacol 2016;182:122-8. [DOI:10.1016/j.jep.2016.02.002]
7. Suffness M, Douros J. Current status of the NCI plant and animal product program. J Nat Prod 1982;45(1):1-14. [DOI:10.1021/np50019a001]
8. Saad B, Azaizeh H, Abu-Hijleh G, Said O. Safety of traditional Arab herbal medicine. Evid-Based Complemen Altern Med 2006;3(4):433-9. [DOI:10.1093/ecam/nel058]
9. Yakubu M, Akanji M, Oladiji A. Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem in male albino rats. Asian J Androl 2005;7(4):399-404. [DOI:10.1111/j.1745-7262.2005.00052.x]
10. Obici S, Otobone FJ, da Silva Sela VR, Ishida K, da Silva JC, Nakamura CV, et al. Preliminary toxicity study of dichloromethane extract of Kielmeyera coriacea stems in mice and rats. J Ethnopharmacol 2008;115(1):131-9. [DOI:10.1016/j.jep.2007.09.013]
11. Gram H. Summary of WHO guidelines for the assessment of herbal medicines. Switzerland: World Health Organization. 1993:13-4.
12. Saggu S, Divekar H, Gupta V, Sawhney R, Banerjee P, Kumar R. Adaptogenic and safety evaluation of seabuckthorn (Hippophae rhamnoides) leaf extract: a dose dependent study. Food Chem Toxicol 2007;45(4):609-17. [DOI:10.1016/j.fct.2006.10.008]
13. Malomo S. Toxicological implications of ceftriaxone administration in rats. Proteins 2000;5:34-8.
14. Akanji MA. A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular system. [Phd Thesis] University of Ife, Ile Ife Nigeria,1986.
15. Wright PJ, Plummer DT. The use of urinary enzyme measurements to detect renal damage caused by nephrotoxic compounds. Biochem Pharmacol 1974;23(1):65-73. [DOI:10.1016/0006-2952(74)90314-1]
16. Malbica J, Hart L. Effect of adenosine triphosphate and some anti-inflammatory agents on a purified lysosomal fraction having high acid phosphatase and labile β-glucuronidase activity. Biochem Pharmacol 1971;20(8):2017-26. [DOI:10.1016/0006-2952(71)90401-1]
17. Mayne PD. Clinical Chemistry in Diagnosis and Treatment. 6th Ed. Arnold international students,1998.p.196-205.
18. Kaplan LA, Pesce AJ. Clinical chemistry: Theory, Analysis and correlation. 3rd Ed. A Harcourt Health Science Company, Mosby, London ,1996.P. 140-52.
19. Ganong WF. Review of medical physiology. 20th Ed. London: Lange Medical Books/McGraw-Hill Medical Publishing Division, 2001. p.414–7.
20. Moudgil KD, Narang BS. The liver and the biliary system. In: Talwar GP, Srivastava LM, Moudgil KD (eds.) Textbook of biochemistry and human biology. 2nd ed. New Delhi: Prentice-Hall of India Pvt Ltd, 1989. p.271–3.
21. Naganna B. Plasma proteins. In: Tawlar GP, Srivastava LM, Moudgils KD (eds.) Textbook of biochemistry and human biology. 2nd ed. New Delhi: Prentice- Hall of India Pvt Ltd, 1989. p.172–81.

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2021 CC BY-NC 4.0 | Iranian Journal of Toxicology

Designed & Developed by : Yektaweb