Write your message
Volume 11, Issue 3 (May-June 2017)                   IJT 2017, 11(3): 47-50 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zargari F, Tabaghchi Saeedy H. Effects of Vitamin C on Paraoxonase1 Arylesterase Activity in Rats Exposed to Arsenic. IJT. 2017; 11 (3) :47-50
URL: http://ijt.arakmu.ac.ir/article-1-546-en.html
1- Department of Medical Science, Marand Branch, Islamic Azad University, Marand, Iran. , zargarifkb@gmail.com
2- Department of Biology, Ahar Branch, Islamic Azad University, Ahar, Iran.
Abstract:   (4021 Views)

Background: Arsenic as an environmental toxicant is able to induced oxidative stress. The present study aimed to evaluate of vitamin C supplementation on the paraoxonase1 (PON1) arylesterase activity (responsible for hydrolysing lipid-peroxides) in rat exposed to arsenic.

Methods: The study was conducted in the Faculty of Veterinary Medicine, Tabriz Islamic Azad University, Tabriz, Iran in 20015. Thirty-two adult male rats weighing 200-350 g were randomly divided into 4 groups: group I (Normal healthy rat), group II [treated with sodium arsenite (100 ppm in drinking water)], group III [treated with vitamin C supplementation (200 mg/kg via gavage)] group IV (treated with sodium arsenite and vitamin c 100 ppm and 200 mg/kg respectively).

Results: Vitamin C supplementation increased paraoxonase1 (PON1) arylesterase activity (P<0.05) but had no significant effect on serum levels of HDL, triglyceride and cholesterol.

Conclusion: PON1 arylesterase activity is independent of changes in HDL and lipid profile, and vitamin C with different mechanisms such as radical scavenging activity and reduction of oxidative stress leads to increased activity of paraoxonase.

Full-Text [PDF 200 kb]   (1036 Downloads)    
Type of Study: Research | Subject: Special

1. Heidari Shayesteh T, Ranjbar A. Oxidative stress in jobs with exposure to xenobiotics. Occup Med Q J. [Review Article] 2013;4(4):75-91.
2. Zargari F, Ghorbanihaghjo A, Babaei H, Farajnia S, Roodbari NH. The effect of hydroalcoholic extract of Nasturtium officinale R. Br on antioxidant status and DNA damage in liver and kidney rats exposed to arsenic. Med J Tabriz Uni Med Sci 2014;36(3):44.
3. Rafati RM, Moghadamnia A. Arsenic compounds toxicity. J Babol Univ Med Sci 2013; 15: 51-68.
4. Kaya Y, Cebi A, Soylemez N, Demir H, Alp HH, Bakan E. Correlations between oxidative DNA damage, oxidative stress and coenzyme Q10 in patients with coronary artery disease. Int J Med Sci 2012;9(8):621-6. [DOI:10.7150/ijms.4768]
5. Afolabi OK, Wusu AD, Ogunrinola OO, Abam EO, Babayemi DO, Dosumu OA, et al. Paraoxonase 1 activity in subchronic low‐level inorganic arsenic exposure through drinking water. Environ Toxicol 2016; 31: 154–62. [DOI:10.1002/tox.22030]
6. Litvinov D, Mahini H, Garelnabi M. Antioxidant and Anti-Inflammatory Role of Paraoxonase 1: Implication in Arteriosclerosis Diseases. North Am J Med Sci 2012;4 (11):523-32. [DOI:10.4103/1947-2714.103310]
7. Takaeidi MR, Jahangiri A, Khodayar MJ, Siahpoosh A, Yaghooti H, Rezaei S, Salecheh M, Mansourzadeh Z. The Effect of Date Seed (Phoenix dactylifera) Extract on Paraoxonase and Arylesterase Activities in Hypercholesterolemic Rats. Jundishapur J Nat Pharm Prod 2014; 9 (1): 30-4. [DOI:10.17795/jjnpp-10368]
8. Erdem FH, Karatay S, Yildirim K, Kiziltunc A. Evaluation of serum paraoxonase and arylesterase activities in ankylosing spondylitis patients. Clinics. 2010;65(2):175-9. [DOI:10.1590/S1807-59322010000200009]
9. Unal E, Eris C, Kaya B, Uzun H, Cavdar F, Yildar M, et al. Paraoxonase and arylesterase activities, lipid profile, and oxidative damage in experimental ischemic colitis model. Gastroenterology Res Pract 2012;2012.
10. Li WF, Sun CW, Cheng TJ, Chang KH, Chen CJ, Wang SL. Risk of carotid atherosclerosis is associated with low serum paraoxonase (PON1) activity among arsenic exposed residents in Southwestern Taiwan. Toxicol Appl Pharmacol 2009;236 (2):246-53 [DOI:10.1016/j.taap.2009.01.019]
11. Sunga MNS, Pascual A. Effect of Ascorbic Acid on Dyslipidemia. (a study among Philippine Heart Center Employees) . Phil Heart Center J 2012; 16 (2):7-11.
12. Gaur GS, Dixit AK. Effect of vitamin C on serum lipid profile in normal human subjects : a study . Int J Curr Res 2011; 3: 286-88.
13. Gursu MF , Onderci M, Gulcu F , Sahin K. Effects of vitamin C and folic acid supplementation on serum paraoxonase activity and metabolites induced by heat stress in vivo. Nutr Res 2004; 24:157-64. [DOI:10.1016/j.nutres.2003.11.008]
14. Begcevic I, Simundic AM, Nikolac N, Dobrijevic S, Rajkovic MC, Tesija-Kuna A. Can cranberry extract and vitaminC+Zn supplements effect the in vivo activity of paraoxonase 1, antioxidant potential, and lipid status? Clin Lab 2013; 59:1053-60. [DOI:10.7754/Clin.Lab.2012.121005]
15. Yagi K. Lipid peroxides and related radicals in clinical medicine. In: Free Radicals in Diagnostic Medicine. New York: Plenum Press; 1994.p. 1-15. [DOI:10.1007/978-1-4615-1833-4_1]
16. Afolabi OK, Wusu AD, Ogunrinola OO, Abam EO, Babayemi DO, Dosumu OA, et al. Arsenic-induced dyslipidemia in male albino rats: comparison between trivalent and pentavalent inorganic arsenic in drinking water. BMC Pharmacol Toxicol 2015;16(1):15. [DOI:10.1186/s40360-015-0015-z]
17. Usoh I, Akpan E, Etim E, Farombi E. Antioxidant actions of dried flower extracts of Hibiscus sabdariffa L. on sodium arsenite-induced oxidative stress in rats. Pak J Nutr 2005;4(3):135-41. [DOI:10.3923/pjn.2005.135.141]
18. Singh S, Rana SV. Amelioration of arsenic toxicity by L-Ascorbic acid in laboratory rat. J Environ Biol 2007;28 (2):377-84.
19. Flora SJ, Bhadauria S, Kannan GM, Singh N. Arsenic induced oxidative stress and the role of antioxidant supplementation during chelation: a review. J Environ Biol 2007;28 (2):333-47.
20. Hassanshahi J, Hassanshahi GH, Zamani M, Hakimizadeh E, Soleimani M. Comparing the therapeutic effects of vitamin C and CoQ10 in reducing the number of damaged cells in mice hippocampus following ischemia-reperfusion. J Mazandaran Univ Med 2012; 22: 2-13.
21. - Gholipour Baradari A, Emami Zeydi A, Khademlu M, Naghshvar F, Razavi Baladehi M, Espahbodi F. The effect of intravenous vitamin C on the level of CRP in Hemodialysis patients. J Mazandaran Univ Med Sci 2011; 20: 55-61.
22. Dortaj H , Yadegari M , Hosseini Sharif Abad M , Abbasi Sarcheshmeh A , Anvari M. Effects of Acrylamide and Vitamin C on Histological Changes and Stereological Parameters of Cerebellum in Rat Offsprings. Shefaye Khatam 2014;2 : 9-18. [DOI:10.18869/acadpub.shefa.2.3.9]
23. Salehi M, Jafari M, Asgari A. Response of liver antioxidant defense system to vitamins E and C against. Sabzevar Univ Med Sci 2015; 21:1081-9.
24. Eteng MU, Ibekwe HA, Amatey TE, Bassey BJ, Uboh FU, Owu DU. Effect of vitamin C on serum lipids and electrolyte profile of albino Wistar rats. Niger J Physiol Sci 2006;21(1-2):15-9.
25. - Afkhami-Ardekani M, Shojaoddiny-Ardekani A. Effect of vitamin C on blood glucose, serum lipids & serum insulin in type 2 diabetes patients. Indian J Med Res 2007; 126:471-4.
26. Dashti GhR, Behdadipour ZA, Mardani M, Farzan A, Vahedi P. Effect of Ascorbic Acid on blood serum lipids in male rabbits fed high –cholesterol diet. IUMS 2006;24:57-60.
27. Ferretti G , Bacchetti T , Masciangelo S , Pallotta G. Lipid peroxidation in hemodialysis patients:effect of vitamin C supplementation.Clin Biochem 2008; 41:381-6. [DOI:10.1016/j.clinbiochem.2007.12.011]
28. Afolabi OK, Wusu AD, Ogunrinola OO, Abam EO, Babayemi DO, Dosumu OA, Onunkwor OB, Balogun E M, Odukoya O, Ademuyiwa O. Paraoxonase 1 activity in subchronic low-level inorganic arsenic exposure through drinking water. Environ Toxicol 2014.
29. Ghatreh Samani K, Farrokhi E, Hashemzadeh Chaleshtory M, Azadegan F. Paraoxonase-1 L55M polymorphism with fatty acid composition of phospholipids in high-density lipoproteins. Tehran Univ Med J 2012; 70:7-14.

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2021 CC BY-NC 4.0 | Iranian Journal of Toxicology

Designed & Developed by : Yektaweb