Write your message
Volume 11, Issue 6 (November-December 2017)                   IJT 2017, 11(6): 13-20 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bashiri Dezfouli A, Salar-Amoli J, Yazdi M, Ali-Esfahani T, Barin A. Evaluation of the Antioxidant Activities and Cytotoxicities of Selected Medicinal Herbs Using Human Hepatoma Cell Line (Hepg2) . IJT. 2017; 11 (6) :13-20
URL: http://ijt.arakmu.ac.ir/article-1-587-en.html
1- PhD of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2- DVSc of Clinical Pathology, Toxicology and Animal Poisoning Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
3- DVM, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
4- MSc Student of Mycology, Toxicology and Animal Poisoning Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
5- PhD of Pathobiology, Toxicology and Animal Poisoning Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Abstract:   (2267 Views)
Background: The aim of this study was to evaluate and compare the hepatotoxicity as well as antioxidants activities of hydromethanolic extract of six common traditional species (Origanum vulgar, Pterocarya fraxinifolia, Ferula assafoetida, Artemisia dracunculus, Rosmarinus officinalis, and Valerian officinalis) in order to find a safe antioxidant source.
Methods: This research project was performed at Toxicology and Animal Poisoning Research Center, University of Tehran (Tehran, Iran), in 2016. The HepG2 cells viability was examined by LDH and MTT techniques following treatment with different concentrations of selected herbal hydroethanolic extracts for 72 h. Furthermore, assessment of antioxidative properties of the extracts was carried out by various scavenging models including DPPH and FRAP.
Results: The highest cytotoxicity was displayed by F. asafoetida extract (IC50= 67.3 µg/ml). R. Officinalis and A. dracunculus extracts were noted as non-toxic due to the high effective dose. Interestingly, V. officinalis extract indicated stimulating effects on cell growth/ proliferation with ED50 values of 20.9 µ/ml. The highest and lowest antioxidant capacities refer to R. officinalis and V. officials. In DPPH assay, the IC50 value of R. officinalis and V. officinalis extracts was found to be 39.82 and 371.77 µg/ml, respectively. FRAP values of R. officinalis and V. officials were 2754.07 and 561.14 µM/g, respectively.
Conclusion: This study identified R. officinalis extract as a natural non-toxic agent with remarkable antioxidant potential in phytomedicine.
Full-Text [PDF 486 kb]   (959 Downloads)    
Type of Study: Research | Subject: General

1. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 2001;109(1):69-70. [DOI:10.1289/ehp.01109s169]
2. Spencer PS, Ludolph AC, Kisby GE. Neurologic diseases associated with use of plant components with toxic potential. Environ Res 1993;62(1):106-13. [DOI:10.1006/enrs.1993.1095]
3. Rodriguez-Fragoso L, Reyes-Esparza J, Burchiel SW, Herrera-Ruiz D, Torres E. Risks and benefits of commonly used herbal medicines in Mexico. Toxicol Appl Pharmacol 2008;227(1):125-35. [DOI:10.1016/j.taap.2007.10.005]
4. Saad B, Dakwar S, Said O, Abu-Hijleh G, Battah FA, Kmeel A, et al. Evaluation of medicinal plant hepatotoxicity in co-cultures of hepatocytes and monocytes. Evid Based Complement Alternat Med 2006;3(1):93-8. [DOI:10.1093/ecam/nel002]
5. Frei B. Natural antioxidants in human health and disease: Academic Press; 2012.
6. Carocho M, Ferreira IC. A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem Toxicol 2013;51:15-25. [DOI:10.1016/j.fct.2012.09.021]
7. Chirinos R, Pedreschi R, Rogez H, Larondelle Y, Campos D. Phenolic compound contents and antioxidant activity in plants with nutritional and or medicinal properties from the Peruvian Andean region. Ind Crops Prod 2013;47:145-52. [DOI:10.1016/j.indcrop.2013.02.025]
8. Sultana B, Anwar F, Ashraf M. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Mol 2009;14(6):2167-80. [DOI:10.3390/molecules14062167]
9. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65(1-2):55-63. [DOI:10.1016/0022-1759(83)90303-4]
10. Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TCd, Coube CS, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 2001;15(2):127-30. [DOI:10.1002/ptr.687]
11. Benzie IF, Strain J. Ferric reducing antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;299:15-27. [DOI:10.1016/S0076-6879(99)99005-5]
12. Vivekananthan DP, Penn MS, Sapp SK, Hsu A, Topol EJ. Use of antioxidant vitamins for the prevention of cardiovascular disease: meta-analysis of randomised trials. Lancet 2003;361(9374):2017-23. [DOI:10.1016/S0140-6736(03)13637-9]
13. Moon J-K, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem 2009;57(5):1655-66. [DOI:10.1021/jf803537k]
14. Kaur S, Mondal P. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. J Microbiol Exp 2014;1(1):1-6. [DOI:10.15406/jmen.2014.01.00005]
15. Galvao J, Davis B, Tilley M, Normando E, Duchen MR, Cordeiro MF. Unexpected low-dose toxicity of the universal solvent DMSO. FASEB J 2014;28(3):1317-30. [DOI:10.1096/fj.13-235440]
16. Conforti F, Perri V, Menichini F, Marrelli M, Uzunov D, Statti GA, et al. Wild Mediterranean dietary plants as inhibitors of pancreatic lipase. Phytother Res 2012;26(4):600-4. [DOI:10.1002/ptr.3603]
17. Dias PC, Foglio MA, Possenti A, de Carvalho JE. Antiulcerogenic activity of crude hydroalcoholic extract of Rosmarinus officinalis L. J Ethnopharmacol 2000;69(1):57-62. [DOI:10.1016/S0378-8741(99)00133-6]
18. Bakırel T, Bakırel U, Keleş OÜ, Ülgen SG, Yardibi H. In vivo assessment of antidiabetic and antioxidant activities of rosemary (Rosmarinus officinalis) in alloxan-diabetic rabbits. J Ethnopharmacol 2008;116(1):64-73. [DOI:10.1016/j.jep.2007.10.039]
19. 19.Aceites 2010;61(3):244-50. [DOI:10.3989/gya.110809]
20. Ebrahimzadeh M, Nabavi S. Essential oil Composition and Antioxidant Activity of Pterocaryafivaxinifolia. Pak J Biol Sci 2009;12(13):957-63. [DOI:10.3923/pjbs.2009.957.963]
21. Rezvani ME, Roohbakhsh A, Allahtavakoli M, Shamsizadeh A. Anticonvulsant effect of aqueous extract of Valeriana officinalis in amygdala-kindled rats: possible involvement of adenosine. J Ethnopharmacol 2010;127(2):313-8. [DOI:10.1016/j.jep.2009.11.002]
22. Weinöhrl SE. Screening of various extracts of Artemisia Dracunculus L. for antidiabetic activity in rats: uniwien; 2010.
23. Carvalho RN, Moura LS, Rosa PT, Meireles MAA. Supercritical fluid extraction from rosemary (Rosmarinus officinalis): Kinetic data, extract\'s global yield, composition, and antioxidant activity. J Supercritical Fluids 2005;35(3):197-204. [DOI:10.1016/j.supflu.2005.01.009]
24. Sotelo-Felix J, Martinez-Fong D, Muriel P, Santillan R, Castillo D, Yahuaca P. Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride-induced acute hepatotoxicity in the rat. J Ethnopharmacol 2002;81(2):145-54. [DOI:10.1016/S0378-8741(02)00090-9]
25. Iranshahy M, Iranshahi M. Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gum-resin)-A review. J Ethnopharmacol 2011;134(1):1-10. [DOI:10.1016/j.jep.2010.11.067]
26. Iranshahi M, Kalategi F, Rezaee R, Shahverdi AR, Ito C, Furukawa H, et al. Cancer chemopreventive activity of terpenoid coumarins from Ferula species. Planta med 2008;74(02):147-50. [DOI:10.1055/s-2008-1034293]
27. Goudah A, Abdo-El-Sooud K, Yousef MA. Acute and subchronic toxicity assessment model of Ferula assa-foetida gum in rodents. Vet World 2015;8(5):584. [DOI:10.14202/vetworld.2015.584-589]
28. Bagheri SM, Yadegari M, Mirjalily A, Rezvani ME. Evaluation of toxicity effects of asafetida on biochemical, hematological, and histological parameters in male wistar rats. Toxicol Int 2015;22(1):61-2. [DOI:10.4103/0971-6580.172258]
29. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001;49(11):5165-70. [DOI:10.1021/jf010697n]
30. Chen C, Hwang J, Lee W, Chiang H, Lin J, Chen H. Search for anti-tumor agents from Chinese herbs. I. Anti-tumor screening method. Chinese Med J (Taipei) 1988;41(3):177-84.
31. Grbović F, Stanković MS, Ćurčić M, Đorđević N, Šeklić D, Topuzović M, et al. In vitro cytotoxic activity of Origanum vulgare L on HCT-116 and MDA-MB-231 cell lines. Plants 2013;2(3):371-8. [DOI:10.3390/plants2030371]
32. Hussain AI. Characterization and biological activities of essential oils of some species of Lamiaceae. PhD Thesis, Faculty Of Sciences, University Of Agroculture, Faisalbad, Pakistan. 2009.
33. Khezrilu Bandli J, Heidari R. The Evaluation of Antioxidant Activities and Phenolic Compounds in Leaves and Inflorescence of Artemisia dracunculus L. by HPLC. J Med Plants 2014;3(51):41-50.
34. Hui-lian W, Dong-fang Z, Zhao-feng L, Yang L, Qian-rong L, Yu-zhen W. In vitro study on the genotoxicity of dichloromethane extracts of valerian (DEV) in human endothelial ECV304 cells and the effect of vitamins E and C in attenuating the DEV-induced DNA damages. Toxicol Appl Pharmacol 2003;188(1):36-41. [DOI:10.1016/S0041-008X(03)00017-6]
35. Horváthová E, Slameňová D, Maršálková L, Šramková M, Wsólová L. Effects of borneol on the level of DNA damage induced in primary rat hepatocytes and testicular cells by hydrogen peroxide. Food Chem Toxicol 2009;47(6):1318-23. [DOI:10.1016/j.fct.2009.03.002]

Add your comments about this article : Your username or Email:

© 2020 All Rights Reserved | Iranian Journal of Toxicology

Designed & Developed by : Yektaweb