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Volume 11, Issue 6 (November-December 2017)
IJT 2017, 11(6): 7-12 |
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Bahtouee M, Fatemikia H, Pourkhalili K, Kim E, Rahimi H R, Tavosi Z, et al . Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in Rats. IJT 2017; 11 (6) :7-12
URL: http://ijt.arakmu.ac.ir/article-1-599-en.html
URL: http://ijt.arakmu.ac.ir/article-1-599-en.html
Mehrzad Bahtouee1 
, Hossein Fatemikia2 , Khalil Pourkhalili3 , Euikyung Kim4 , Hamid Reza Rahimi5 , Zahra Tavosi1 , Abdol hamid Esmaili5 , Abdollah Hajivandi6 , Yaghoob Hassan7 , Ramin Seyedian * 8
, Hossein Fatemikia2 , Khalil Pourkhalili3 , Euikyung Kim4 , Hamid Reza Rahimi5 , Zahra Tavosi1 , Abdol hamid Esmaili5 , Abdollah Hajivandi6 , Yaghoob Hassan7 , Ramin Seyedian * 8
1- Department of Internal Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
2- Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Department of Physiology, Bushehr University of Medical Sciences, Bushehr, Iran.
4- Department of Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea.
5- Department of Pathology, Bushehr University of Medical Sciences, Bushehr, Iran.
6- Department of Biostatistics, Bushehr University of Medical Sciences, Bushehr,Iran.
7- MSc of Nutrition, Research Center, Bushehr University of Medical Science, Bushehr, Iran.
8- Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran. ,raminseyedian@gmail.com
2- Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Department of Physiology, Bushehr University of Medical Sciences, Bushehr, Iran.
4- Department of Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea.
5- Department of Pathology, Bushehr University of Medical Sciences, Bushehr, Iran.
6- Department of Biostatistics, Bushehr University of Medical Sciences, Bushehr,Iran.
7- MSc of Nutrition, Research Center, Bushehr University of Medical Science, Bushehr, Iran.
8- Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran. ,
Abstract: (5181 Views)
Background: Bleomycin-induced lung fibrosis has been accepted as an animal model for fibrosis in rats. The aim of this study was to evaluate the effects of saffron aqueous extract on this disorder paving the way for more investigation in treating idiopathic pulmonary fibrosis in human.
Methods: Male Wistar rats (250–300 gr) were instilled a single dose of bleomycin (5 mg/kg) via intratracheal tube (n=6) in 2015. Sham group received normal saline. Saffron aqueous extract (50 mg/kg and 100 mg/kg) were given orally in two different treated groups with bleomycin for 28 days. Lung Indices was calculated at the end of this experiment. Lung segments fixed in 10% formaldehyde were used for pathological preparation with Hematoxylin & Eosin and trichrome staining.
Results: The body weight was decreased and lung Indices increased in bleomycin group (P<0.5). Bleomycin administration increased myeloperoxidase, malondialdehyde and finally TNF-α in lung tissue homogenates (P<0.05) compared with sham group. The fibrotic process and thickening of alveolar septa in treated rats with bleomycin were increased by H&E and Masson Trichrome staining. Saffron treatment (50 and 100 mg/kg) attenuated the increase in MDA (264.43±10.4 nmol/g by the higher dose versus 378.4±18.1nmol/g), MPO (0.19±0.03 and 0.13± 0.04 IU/ml versus 0.39.2±0.05 IU/ml) and TNF-α level (18.42±3.7 ng/ml and14.31±3.6 ng /ml versus 35.32±4.2) in lung homogenates compared to bleomycin group (P<0.05). It decreased collagen accumulation and alveolar destructive patterns in pulmonary fibrosis.
Conclusion: This study introduces saffron as novel anti-fibrotic agent against bleomycin-induced fibrosis due to histological examinations and preventive effects on destructive enzyme release in rats.
Methods: Male Wistar rats (250–300 gr) were instilled a single dose of bleomycin (5 mg/kg) via intratracheal tube (n=6) in 2015. Sham group received normal saline. Saffron aqueous extract (50 mg/kg and 100 mg/kg) were given orally in two different treated groups with bleomycin for 28 days. Lung Indices was calculated at the end of this experiment. Lung segments fixed in 10% formaldehyde were used for pathological preparation with Hematoxylin & Eosin and trichrome staining.
Results: The body weight was decreased and lung Indices increased in bleomycin group (P<0.5). Bleomycin administration increased myeloperoxidase, malondialdehyde and finally TNF-α in lung tissue homogenates (P<0.05) compared with sham group. The fibrotic process and thickening of alveolar septa in treated rats with bleomycin were increased by H&E and Masson Trichrome staining. Saffron treatment (50 and 100 mg/kg) attenuated the increase in MDA (264.43±10.4 nmol/g by the higher dose versus 378.4±18.1nmol/g), MPO (0.19±0.03 and 0.13± 0.04 IU/ml versus 0.39.2±0.05 IU/ml) and TNF-α level (18.42±3.7 ng/ml and14.31±3.6 ng /ml versus 35.32±4.2) in lung homogenates compared to bleomycin group (P<0.05). It decreased collagen accumulation and alveolar destructive patterns in pulmonary fibrosis.
Conclusion: This study introduces saffron as novel anti-fibrotic agent against bleomycin-induced fibrosis due to histological examinations and preventive effects on destructive enzyme release in rats.
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