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Volume 11, Issue 6 (November-December 2017)                   IJT 2017, 11(6): 43-47 | Back to browse issues page

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Shirmohammadi M, Ronagh M T, Salamat N, Movahedinia A, Hamidian G. Response of Melanomacrophage Centers in Yellowfin Seabream (Acanthopagrus latus) Immune Organs as an Immunohistological Biomarker in Short-term Exposure to Phenanthrene. IJT 2017; 11 (6) :43-47
URL: http://ijt.arakmu.ac.ir/article-1-611-en.html
Response of Melanomacrophage Centers in Yellowfin Seabream (Acanthopagrus latus) Immune Organs as an Immunohistological Biomarker in Short-term Exposure to Phenanthrene
Mehrnaz Shirmohammadi1 , Mohammad Taghi Ronagh1 , Negin Salamat1 , Abdolali Movahedinia1 , Gholamreza Hamidian2
1- Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
2- Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Abstract:   (4035 Views)
Background: The aim of this study was to assess the changes in plasma levels of antioxidant enzymes and melano-macrophage centers (MMCs) in immune tissues as biomarkers of yellowfin seabream (Acanthopagrus latus) exposed to phenanthrene (Phe) for 14 days.
Methods: The research was carried out at Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran in Jan 2016. Thirty-six immature fish were intraperitoneally injected with coconut oil (10 μl/g-bw) containing Phe (70mg/kg-bw) and compared with control group (without injection). Then tissue and blood samples were obtained at 1, 4, 7 and 14 d after injection.
Results: The SOD and CAT activity showed a significant decrease in fish exposed to Phe up to day 7, then activity increased at day 14 in Phe-treated fish (P<0.05). The size and number of MMCs in treated fish in spleen and head kidney were higher than control.
Conclusion: Changes in these parameters (SOD, CAT, and MMCs) might be used as useful biomarkers for evaluating immunosuppressive Phe in fish.
Keywords: Antioxidant Enzymes, Melano-Macrophage Centers, Phenanthrene, Yellowfin Seabream
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Type of Study: Research | Subject: General
References
1. Hannam ML, Bamber SD, Galloway TS, Moody AJ, Jones MB. Effects of the model PAH phenanthrene on immune function and oxidative stress in the haemolymph of the temperate scallop Pecten maximus. Chemosphere 2010;78(7):779-84. [DOI:10.1016/j.chemosphere.2009.12.049]
2. Sun Y, Yu H, Zhang J, Yin Y, Shi H, Wang X. Bioaccumulation, depuration and oxidative stress in fish Carassius auratus under phenanthrene exposure. Chemosphere 2006;63(8):1319-27. [DOI:10.1016/j.chemosphere.2005.09.032]
3. Sinaei M, Mashinchian A. Polycyclic aromatic hydrocarbons in the coastal sea water, the surface sediment and Mudskipper Boleophthalmus dussumieri from coastal areas of the Persian Gulf: source investigation, composition pattern and spatial distribution. J Environ Health Sci Eng 2014;12(1):59-60. [DOI:10.1186/2052-336X-12-59]
4. Mikula P, Modra H, Nemethova D, Groch L, Svobodova Z. Effects of Subchronic Exposure to LASSO MTX®(Alachlor 42% W/V) on Hematological Indices and Histology of the Common Carp, Cyprinus carpio L. Bull Environ Contam Toxicol 2008;81(5):475-9. [DOI:10.1007/s00128-008-9500-z]
5. Suresh N. Effect of cadmium chloride on liver, spleen and kidney melano macrophage centres in Tilapia mossambica. J Environ Biol 2009;30(4):505-8.
6. Vijayavel K, Gomathi R, Durgabhavani K, Balasubramanian M. Sublethal effect of naphthalene on lipid peroxidation and antioxidant status in the edible marine crab Scylla serrata. Mar Pollut Bull 2004;48(5):429-33. [DOI:10.1016/j.marpolbul.2003.08.017]
7. Pathiratne A, Hemachandra CK. Modulation of ethoxyresorufin O-deethylase and glutathione S-transferase activities in Nile tilapia (Oreochromis niloticus) by polycyclic aromatic hydrocarbons containing two to four rings: implications in biomonitoring aquatic pollution. Ecotoxicol 2010;19(6):1012-8. [DOI:10.1007/s10646-010-0482-3]
8. Phalen LJ, Köllner B, Leclair LA, Hogan NS, van den Heuvel MR. The effects of benzo pyrene on leucocyte distribution and antibody response in rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 2014;147:121-8. [DOI:10.1016/j.aquatox.2013.12.017]
9. Vossoughi M, Moslehi P, Alemzadeh I. Research note some investigation on bioremediation of sediment in persian gulf coast. Int J Eng 2005;18(1):45-53.
10. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72(1-2):248-54. [DOI:10.1016/0003-2697(76)90527-3]
11. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47(3):469-74. [DOI:10.1111/j.1432-1033.1974.tb03714.x]
12. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6. [DOI:10.1016/S0076-6879(84)05016-3]
13. Manrique W, Silva Claudiano Gd, Petrillo T, Castro MP, Figueiredo P, Belo A, et al. Response of splenic melanomacrophage centers of Oreochromis niloticus (Linnaeus, 1758) to inflammatory stimuli by BCG and foreign bodies. Applied Ichthyol 2014;30(5):1001-6. [DOI:10.1111/jai.12445]
14. Sayed A, Younes HA. Melanomacrophage centers in Clarias gariepinus as an immunological biomarker for toxicity of silver nanoparticles. J Microsc Ultrastructur 2017;5(2):97-104. [DOI:10.1016/j.jmau.2016.07.003]
15. Kurtović B, Teskeredžić E, Teskeredžić Z. Histological comparison of spleen and kidney tissue from farmed and wild European sea bass (Dicentrarchus labrax L.). Acta Adriat 2008;49(2):147-54.
16. Blazer V, Wolke R. Ceroid deposition, retinal degeneration and renal calcium oxalate crystals in cultured clownfish, Amphiprion ocellaris. J Fish Dis 1983;6(4):365-76. [DOI:10.1111/j.1365-2761.1983.tb00088.x]
17. Obach A, Quentel C, Baudin Laurencin F. Effects of alpha-tocopherol and dietary oxidized fish-oil on the immune-response of Sea Bass Dicentrarchus labrax. Dis Aqu Org 1993; 15: 175-85. [DOI:10.3354/dao015175]
18. Gaber HS. Fish health as a biomarker for the condition of Lake Nasser (Review article). J Appl Sci Res 2013; 9(11): 5794-810.
19. Dias D, Maiorino F, Ranzani-Paiva M, Ishikawa N, Lombardi J, Ferreira J, et al. Avaliação histopatológica do baço, coração e encéfalo de tilápia Oreochromis niloticus (linnaeus, 1758) exposta ao cloreto de mercúrio. Bol Inst Pesca 2007;33(2):213-20.
20. Sinhorin VDG, Sinhorin AP, dos Santos Teixeira JM, Miléski KML, Hansen PC, Moreira PSA, et al. Effects of the acute exposition to glyphosate-based herbicide on oxidative stress parameters and antioxidant responses in a hybrid Amazon fish surubim (Pseudoplatystoma sp). Ecotoxicol Environ Saf 2014;106:181-7. [DOI:10.1016/j.ecoenv.2014.04.040]
21. Pan L, Ren J, Zheng D. Effects of benzo (a) pyrene exposure on the antioxidant enzyme activity of scallop Chlamys farreri. J Ocean Limnol 2009;27(1):43-53. [DOI:10.1007/s00343-009-0043-x]
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