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Volume 11, Issue 6 (November-December 2017)
IJT 2017, 11(6): 21-26 |
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Forouhar Vajargah M, Ghafari Farsani H, Gerami M H, Hedayati S A, Nezhadheydari H. Effects of the Prebiotic in Reducing Histopathological Changes and Immune Response of Cyprinuscarpio after Exposer to Abamectin. IJT 2017; 11 (6) :21-26
URL: http://ijt.arakmu.ac.ir/article-1-624-en.html
URL: http://ijt.arakmu.ac.ir/article-1-624-en.html
Mohammad Forouhar Vajargah1 
, Hamed Ghafari Farsani2 , Mohammad Hasan Gerami3 , Seyed Aliakbar Hedayati4 , Hasan Nezhadheydari5
, Hamed Ghafari Farsani2 , Mohammad Hasan Gerami3 , Seyed Aliakbar Hedayati4 , Hasan Nezhadheydari5
1- PhD Student of Aquatic Ecology, Faculty of Natural Resources, University of Guilan, Sowmehsara, Iran.
2- PhD Student of Fisheries, Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
3- PhD of Fisheries, Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
4- Department of Fisheries and Environment, Gorgan University of Agricultural and Natural Resources, Gorgan, Iran.
5- Department of Fisheries, College of Natural Resources, University of Tehran, Karaj, Iran.
2- PhD Student of Fisheries, Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
3- PhD of Fisheries, Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
4- Department of Fisheries and Environment, Gorgan University of Agricultural and Natural Resources, Gorgan, Iran.
5- Department of Fisheries, College of Natural Resources, University of Tehran, Karaj, Iran.
Abstract: (4750 Views)
Background: To assess the toxicity of environmental pollutants in fish, there are different physiological parameters including histology. This study aimed to investigate the effect of prebiotic dietary supplement on fish immune response after exposure to toxicant.
Methods: The study was conducted in the Gorgan University of Agricultural and Natural Resources aquaculture Laboratory in 2017. Common carp species were obtained and exposed to 2 ppm, 3 ppm, 6 ppm Abamectin as a toxicant. Prebiotic isomalto-oligosaccharides added to diet with spray method as 1 g/kg and histopathological examinations were done after 60 d experiment. There were no significant differences between aquariums in water quality during the test and no mortality and injuries were observed during accumulation.
Results: The Abamectin caused some lesions such as vacuolization latest, bleeding, necrosis, degeneration of the epithelium, the destruction of the villi in the intestine, destruction of liver cells, ascites, hemorrhage, necrosis and nuclear karyolysis in the liver and lymphocytes penetration and degradation of intestinal epithelium in intestine. Maximum lesions observed in 6-ppm toxicant concentration.
Conclusion: The isomalto-oligosaccharides probiotics was not successful in stimulating the immune system and reducing adverse effect of toxicant in common carp, significantly. However, usage of this prebiotic could be useful in some cases.
Methods: The study was conducted in the Gorgan University of Agricultural and Natural Resources aquaculture Laboratory in 2017. Common carp species were obtained and exposed to 2 ppm, 3 ppm, 6 ppm Abamectin as a toxicant. Prebiotic isomalto-oligosaccharides added to diet with spray method as 1 g/kg and histopathological examinations were done after 60 d experiment. There were no significant differences between aquariums in water quality during the test and no mortality and injuries were observed during accumulation.
Results: The Abamectin caused some lesions such as vacuolization latest, bleeding, necrosis, degeneration of the epithelium, the destruction of the villi in the intestine, destruction of liver cells, ascites, hemorrhage, necrosis and nuclear karyolysis in the liver and lymphocytes penetration and degradation of intestinal epithelium in intestine. Maximum lesions observed in 6-ppm toxicant concentration.
Conclusion: The isomalto-oligosaccharides probiotics was not successful in stimulating the immune system and reducing adverse effect of toxicant in common carp, significantly. However, usage of this prebiotic could be useful in some cases.
References
1. Gibson GR. Fibre and effects on probiotics (the prebiotic concept). Clin Nutr Suppl 2004;1(2):25-31. [DOI:10.1016/j.clnu.2004.09.005]
2. Yousefian M, Amiri MS. A review of the use of prebiotic in aquaculture for fish and shrimp. Afr J Biotechnol 2009;8(25):7313-8.
3. Rycroft C, Jones M, Gibson G, Rastall R. The role of prebiotics in human gut microbiology. Prebiotic oligosaccharides. J Appl Microbiol 2001;91:878-87. [DOI:10.1046/j.1365-2672.2001.01446.x]
4. Gu Q, Yang Y, Jiang G, Chang G. Study on the regulative effect of isomalto-oligosaccharides on human intestinal flora. J Hyg Res 2003;32(1):54-5.
5. Zhang W, Li D, Lu W, Yi G. Effects of isomalto-oligosaccharides on broiler performance and intestinal microflora. Poult Sci 2003;82(4):657-63. [DOI:10.1093/ps/82.4.657]
6. Thitaram S, Chung C-H, Day D, Hinton Jr A, Bailey J, Siragusa G. Isomalto-oligosaccharide increases cecal Bifidobacterium population in young broiler chickens. Poult Sci 2005;84(7):998-1003. [DOI:10.1093/ps/84.7.998]
7. Goffin D, Delzenne N, Blecker C, Hanon E, Deroanne C, Paquot M. Will isomalto-oligosaccharides, a well-established functional food in Asia, break through the European and American market? The status of knowledge on these prebiotics. Crit Rev Food Sci Nutr 2011;51(5):394-409. [DOI:10.1080/10408391003628955]
8. Playne MJ, Crittenden RG. Part II Biotechnology strategies for producing specific food ingridents. In: Shahidi F, Neeser J-R, German JB, editors. Bioprocesses and biotechnology for functional foods and nutraceuticals. CRC Press; 2004. p. 120-1.
9. Mountzouris K, Gilmour S, Rastall R. Continuous production of oligodextrans via controlled hydrolysis of dextran in an enzyme membrane reactor. J Food Sci 2002;67(5):1767-71. [DOI:10.1111/j.1365-2621.2002.tb08720.x]
10. Ringo E, Olsen R, Gifstad T, Dalmo R, Amlund H, HEMRE GI, et al. Prebiotics in aquaculture: a review. Aqucul Nut 2010;16(2):117-36. [DOI:10.1111/j.1365-2095.2009.00731.x]
11. Merrifield DL, Dimitroglou A, Foey A, Davies SJ, Baker RT, Bøgwald J, et al. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquacul 2010;302(1):1-18. [DOI:10.1016/j.aquaculture.2010.02.007]
12. Soleimani N, Hoseinifar SH, Merrifield DL, Barati M, Abadi ZH. Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol 2012;32(2):316-21. [DOI:10.1016/j.fsi.2011.11.023]
13. Hoseinifar SH, Khalili M, Rostami HK, Esteban MÁ. Dietary galactooligosaccharide affects intestinal microbiota, stress resistance, and performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol 2013;35(5):1416-20. [DOI:10.1016/j.fsi.2013.08.007]
14. Hoseinifar SH, Sharifian M, Vesaghi MJ, Khalili M, Esteban MÁ. The effects of dietary xylooligosaccharide on mucosal parameters, intestinal microbiota and morphology and growth performance of Caspian white fish (Rutilus frisii kutum) fry. Fish Shellfish Immunol 2014;39(2):231-6. [DOI:10.1016/j.fsi.2014.05.009]
15. Mo WY, Cheng Z, Choi WM, Lun CH, Man YB, Wong JT, et al. Use of food waste as fish feeds: effects of prebiotic fibers (inulin and mannanoligosaccharide) on growth and non-specific immunity of grass carp (Ctenopharyngodon idella). Environ Sci Pol Res 2015;22(22):17663-71. [DOI:10.1007/s11356-015-4971-z]
16. Ketabi A, Dieleman L, Gänzle M. Influence of isomalto‐oligosaccharides on intestinal microbiota in rats. J Appl Microbiol 2011;110(5):1297-306. [DOI:10.1111/j.1365-2672.2011.04984.x]
17. Zhang C, GUO Y-m, LI W. Effects of Isomalto-Oligosaccharide on the Performance and Gastro-Intestinal Physlological and Biochemical Parameters of Broilers. Chinese J AniSci 2002;38(5):7-9.
18. Hedayati A, Vajargah MF, Yalsuyi AM, Abarghoei S, Hajiahmadyan M. Acute toxicity test of pesticide abamectin on common carp (Cyprinus carpio). J Coast Life Med 2014;2(11):841-4.
19. Dunier M, Siwicki AK. Effects of pesticides and other organic pollutants in the aquatic environment on immunity of fish: a review. Fish Shellfish Immunol 1993;3(6):423-38. [DOI:10.1006/fsim.1993.1042]
20. Naserabad SS, Mirvaghefi A, Gerami MH, Farsani HG. Acute toxicity and behavioral changes of Caspian kutum (Rutilus frisii Kutum Kamensky, 1991) and Caspian roach (Rutilus rutilus caspicus Jakowlew, 1870) exposed to the fungicide hinosan. Afr J Biotechnol 2015;14(20):1737-42. [DOI:10.5897/AJB2015.14494]
21. Shahbazi Naserabad S, Mirvaghefi A, Gerami MH, Ghafari Farsani H. Acute toxicity and behavioral changes of the gold fish (Carassius auratus) exposed to malathion and hinosan. Iran J Toxicol 2015;8(27):1203-8.
22. Vajargah MF, Hedayati A, Yalsuyi AM, Abarghoei S, Gerami MH, Farsani HG. Acute toxicity of Butachlor to Caspian Kutum (Rutilus frisii Kutum Kamensky, 1991). J Environ Treat Tech 2014;2(4):155-7.
23. Abarghoei S, Hedayati SA, Ghafari Farsani H, Gerami MH. Hematological responses of Gold fish (Carassius auratus) to different acute concentrations of Silver Sulfate as a toxicant. Pollution 2015;1(3):247-56.
24. OECD. Guidelines for the Testing of Chemicals. Organization for Economic. 1993.
25. Thanomsit C. Evaluation of abamectin effect on some biochemical constituents and histological alterations in Asian sea bass (Lates calcarifer). Naresuan Uni J SciTech 2016;24(1):72-8.
26. Magnadóttir B. Innate immunity of fish (overview). Fish Shellfish Immunol 2006;20(2):137-51. [DOI:10.1016/j.fsi.2004.09.006]
27. Mallatt J. Fish gill structural changes induced by toxicants and other irritants: a statistical review. Can J Fish Aquat Sci 1985;42(4):630-48. [DOI:10.1139/f85-083]
28. Suvetha L, Ramesh M, Saravanan M. Influence of cypermethrin toxicity on ionic regulation and gill Na+/K+-ATPase activity of a freshwater teleost fish Cyprinus carpio. Environ Toxicol Pharm 2010;29(1):44-9. [DOI:10.1016/j.etap.2009.09.005]
29. El-Said M. Evaluation of Abamectin toxicity on some biochemical constituents and osmoregulation in freshwater fish Oreochromis niloticus (Tilapia niloticus). J Egypt Soci Toxicol 2007;37:1-10.
30. Al-Kahtani MA. Effect of an insecticide abamectin on some biochemical characteristics of Tilapia fish (Oreochromis niloticus). Am J Agr Biol Sci 2011; 6(1): 62-8. [DOI:10.3844/ajabssp.2011.62.68]
31. Ohta A, Ohtsuki M, Baba S, Adachi T, Sakata T, Sakaguchi E. Calcium and magnesium absorption from the colon and rectum are increased in rats fed fructooligosaccharides. J Nutr 1995;125(9):2417-24.
32. Chen H-L, Lu Y-H, Lin J-J, Ko L-Y. Effects of isomalto-oligosaccharides on bowel functions and indicators of nutritional status in constipated elderly men. J Am Coll Nut 2001;20(1):44-9. [DOI:10.1080/07315724.2001.10719013]
33. Cerezuela R, Cuesta A, Meseguer J, Esteban MÁ. Effects of inulin on gilthead seabream (Sparus aurata) innate immune parameters. Fish Shellfish Immunol 2008;24(5):663-8. [DOI:10.1016/j.fsi.2007.10.002]
34. Olsen R, Myklebust R, Kryvi H, Mayhew T, Ringo E. Damaging effect of dietary inulin on intestinal enterocytes in Arctic charr (Salvelinus alpinus). Aquacul Res 2001;32(11):931-4. [DOI:10.1046/j.1365-2109.2001.00626.x]
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
