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Volume 15, Issue 4 (October 2021)
IJT 2021, 15(4): 249-256 |
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Abayomi T A, Tokunbo O S, Ajayi M, Abayomi O A, Ofusori D A. Riboceine Regimen Attenuates Ethanol-induced Neuronal Damage in the Cerebellum of Adult Male Wistar Rats. IJT 2021; 15 (4) :249-256
URL: http://ijt.arakmu.ac.ir/article-1-971-en.html
URL: http://ijt.arakmu.ac.ir/article-1-971-en.html
Taiwo Adekemi Abayomi1 
, Olorunfemi Samuel Tokunbo * 2, Moyinoluwa Ajayi1 , Olawale Ayobami Abayomi3 , David A. Ofusori4
, Olorunfemi Samuel Tokunbo * 2, Moyinoluwa Ajayi1 , Olawale Ayobami Abayomi3 , David A. Ofusori4
1- Department of Anatomy, School of Basic Medical Sciences, College of Health Sciences, Osun State University, Osogbo, Nigeria.
2- Department of Anatomy, School of Basic Medical Sciences, College of Health Sciences, Osun State University, Osogbo, Nigeria. , olorunfemi.tokunbo@uniosun.edu.ng
3- Department of Radiology, School of Clinical Sciences, Ekiti State University, Ekiti State, Nigeria.
4- Department of Anatomy and Cell Biology, School of Basic Medical Sciences, College of Health Sciences, Obafemi Awolowo University, Ile-ife, Nigeria.
2- Department of Anatomy, School of Basic Medical Sciences, College of Health Sciences, Osun State University, Osogbo, Nigeria. , olorunfemi.tokunbo@uniosun.edu.ng
3- Department of Radiology, School of Clinical Sciences, Ekiti State University, Ekiti State, Nigeria.
4- Department of Anatomy and Cell Biology, School of Basic Medical Sciences, College of Health Sciences, Obafemi Awolowo University, Ile-ife, Nigeria.
Abstract: (1652 Views)
Background: Although ethanol exerts its neurotoxic effect on the brain through inflammatory and oxidative processes, the effect of Riboceine on the brain following ethanol neurotoxicity is yet to be elucidated. Therefore, this study was designed to evaluate the effects of riboceine on the cellular, behavioral, and molecular impairments induced by ethanol toxicity in rats.
Methods: A total of 24 male Wistar rats weighing between 160-170 grams were used for the study, and were divided into four groups of six rats each. After completion of the administration of ethanol and riboceine, and testing for motor impairment, the rats were sacrificed. The cerebellum was excised and processed for oxidative stress analyses, based on oxidative stress markers and histological examinations. The immunohistochemical expression of astrocytes in the cerebellum was examined, using Glial Fibrillary Acidic Protein (GFAP) stain.
Results: This study demonstrated that ethanol-induced neurotoxicity in the cerebellum, characterized by increased oxidative stress profile, astrocyte activation, and neuronal death in the cerebellum, especially the Purkinje layer. Necrosis, significant decrease in Superoxide Dismutase (SOD), Catalase (CAT) and Gluathione (GSH) activities (P<0.05) as well as astrogliosis was associated with ethanol treatment. However, riboceine was observed to significantly increase the cerebellar SOD, CAT and GSH activities with significantly reduced Malondialdehyde (MDA) levels (P<0.05). It also attenuated the histomorphological alteration of the cerebellum and reduced the cerebellar astrocytes activation following ethanol-induced neurotoxicity, thus leading to the attenuation of motor impairment.
Conclusion: Riboceine attenuated motor impairment caused by chronic ethanol-induced neurotoxicity, suggestive of its anti-oxidative and anti-inflammatory properties.
Methods: A total of 24 male Wistar rats weighing between 160-170 grams were used for the study, and were divided into four groups of six rats each. After completion of the administration of ethanol and riboceine, and testing for motor impairment, the rats were sacrificed. The cerebellum was excised and processed for oxidative stress analyses, based on oxidative stress markers and histological examinations. The immunohistochemical expression of astrocytes in the cerebellum was examined, using Glial Fibrillary Acidic Protein (GFAP) stain.
Results: This study demonstrated that ethanol-induced neurotoxicity in the cerebellum, characterized by increased oxidative stress profile, astrocyte activation, and neuronal death in the cerebellum, especially the Purkinje layer. Necrosis, significant decrease in Superoxide Dismutase (SOD), Catalase (CAT) and Gluathione (GSH) activities (P<0.05) as well as astrogliosis was associated with ethanol treatment. However, riboceine was observed to significantly increase the cerebellar SOD, CAT and GSH activities with significantly reduced Malondialdehyde (MDA) levels (P<0.05). It also attenuated the histomorphological alteration of the cerebellum and reduced the cerebellar astrocytes activation following ethanol-induced neurotoxicity, thus leading to the attenuation of motor impairment.
Conclusion: Riboceine attenuated motor impairment caused by chronic ethanol-induced neurotoxicity, suggestive of its anti-oxidative and anti-inflammatory properties.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
