Masoomeh Masoomi Karimi, Moslem Jafari Sani, Ali Zaree Mahmudabadi , Asma Jafari Sani, Seyed Reza Khatibi,
Volume 6, Issue 18 (10-2012)
Abstract
Background: Cadmium is one of the most toxic heavy metals in our environment having a very strong ability to accumulate in body organs, especially in kidney. The present study was done to determine the genotoxicity and cytotoxicity in kidneys of rats exposed to cadmium.
Methods: Male rats (n=30), kept in standard conditions were used in this study. The animals were randomly divided into 2 groups (control and treatment). The treatment group was intraperitoneally injected with Cd (300µm/kg) at hours 0, 6, 12, 24, 48. Twenty four hours after the last injection, the rats were sacrificed and their kidneys were obtained. Then oxidative stress markers, malondialdehide (MDA), glutathione (GSH), and superoxide dismutase (SOD), were assayed in homogenized kidney for studying their cytotoxicity. For genotoxicity and DNA damage studies, Comet assay was run on isolated kidney cells. Data analysis was done by t-test and ANOVA using SPSS software version 15.
Results: MDA and GSH concentrations in normal and Cd exposed kidney cells were 287.01±37.30nmol/g.pr and 15.61±3.89µmol/g.pr and 609.24±87.87nmol/g.pr and 28.52±5.22µmol/g.pr, respectively. In addition, SOD activity in normal and Cd exposed kidney cells were 77.75±4.12 and 218.91±5.40 U/mg.pr, respectively. Comet assay results (content comet length, tail length, and head diameter) showed DNA breakage in the treatment group that was stimulated by Cd which was not seen in the control group.
Conclusion: The results demonstrated the genotoxicity effect of Cd on kidney cells as well as the ability of Cd to producing cytotoxicity.
Asmaa Almukhtar, Zaynab Abdulghany, Amal Ali, Teeba Jaafar,
Volume 19, Issue 3 (4-2025)
Abstract
Background: the cellular phone towers were established in Iraq specifically in Baghdad since 2003 after the war. These towers like knife has two sides: one its advantages in helping people contacting the second it spread frequencies of electromagnetic field around the place.
Objective: This cross-sectional study, first study was carried out in Baghdad city and focused to determine wither cellular phone tower was harm to peoples who set under these towers and the distance between their home and a mobile phone base station were linked to health complaints and the DNA impact.
Methods: In the framework of a small panel survey frequently done by an Iraqi center for cancer and medical genetics research/ Mustansiriyah University/ Baghdad/ Iraq, the study's authors used a population-based, cross-sectional analysis in the beginning. First, questions on the impact of mobile phone base stations on fourteen healthy people (eight direct exposure and six away from phone tower) received responses from respondents of all ages and genders. They initially addressed inquiries on their health and the effects of mobile phone base stations. There was a list of ailments mentioned. The closeness of a dwelling to mobile phone base stations and risk perception were two predictors of health complaints found using a multiple linear regression model. The genotoxicity of cell phone tower station radiation was determined using a DNA comet assay.
Results: The participants (volunteers) were grouped into exposed to 3G and 4G towers. The results of genotoxicity for volunteers who were involved in exposure showed type 3 and 4 damage of DNA by comet assay. While the control participants showed type 0-1 which is normal.
Conclusions: The location of towers above residential buildings with dimensions of zero meters and 7-15 meters high poses the risk of accumulating errors in DNA, which may contribute in the future to dangerous situations for individuals.
