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Volume 18, Issue 1 (January 2024)                   IJT 2024, 18(1): 45-51 | Back to browse issues page


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Jimat R T, Ethica S N, Rahmani N, Yulianti S E, Rachmayati R, Nuryati N, et al . Alginate Lyase from Streptomyces olivaceus is a Safe and Effective Antibiofilm in Male Wistar Rats (Rattus norvegicus). IJT 2024; 18 (1) :45-51
URL: http://ijt.arakmu.ac.ir/article-1-1274-en.html
1- Magister Study Program of Clinical Laboratory Science, Postgraduate Program, Universitas Muhammadiyah Semarang
2- Research Center for applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency
3- Research Center for Biosystematics and Evolution, Organization Research of Life Sciences and Environment, National Research and Innovation Agency
4- Deputy for Regional Research and Innovation, National Research and Innovation Agency
5- Research Center for Deep Sea, Earth Sciences and Maritime Research Organization, National Research and Innovation Agency
6- Department of Biomedical Sciences, Faculty of Medicine, Universitas Muhammadiyah Semarang , mayadianr@unimus.ac.id
Abstract:   (503 Views)
Background: Alginate lyase is known to have antibiofilm activity. Toxicity tests for the alginate lyase produced by Streptomyces olivaceus from Serena Kecil Island, North Sulawesi, Indonesia, have never been reported. This research conducted an in vivo toxicity study of the alginate lyase, using male Wistar rats for its development as an oral anti-biofilm agent.
Methods: Twenty male Wistar rats (Rattus norvegicus) were divided equally into four groups of: technical control (TC), which was only given food and drink; negative control (NC), given phosphate buffer saline 1x; experimental group (E1), given alginate lyase at a dose of 0.63 g/kg BW, and (E2), given alginate lyase at a dose of 20.85 g/kg BW. The toxicity tests were carried out for 7 days by observing the following parameters: changes in the body weight and behavior; changes in AST and ALT enzyme levels; and evaluation of macroscopic and microscopic damages to the liver.
Results: After 7 days of treatment, rats in the NC, E1, and E2 groups experienced insignificant weight losses. They also had normal behavior and did not show signs of toxicity or death. The AST and ALT levels in rats, given alginate lyase (E1 and E2) decreased significantly but were still within the normal range.  Alginate lyase also caused an adaptive stress response and degeneration in the liver cells.
Conclusion: Alginate lyase at 0.63 g/kg BW and 20.85 g/kg BW were considered safe in the rats and had the prospect of being developed into an anti-biofilm agent.
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Type of Study: Research | Subject: General

References
1. Founou RC, Founou LL, Essack SY. Clinical and economic impact of antibiotic resistance in developing countries: A systematic review and meta-analysis. PLoS One. 2017;12(12):e0189621. [DOI:10.1371/journal.pone.0189621] [PMID] []
2. Topka-Bielecka G, Dydecka A, Necel A, Bloch S, Nejman-Falenczyk B, Wegrzyn G, et al. Bacteriophage-Derived Depolymerases against Bacterial Biofilm. Antibiotics (Basel). 2021;10(2). [DOI:10.3390/antibiotics10020175] [PMID] []
3. Ethica SN. Bioprospection of alginate lyase from bacteria associated with brown algae Hydroclathrus sp. as antibiofilm agent: a review. ACL Bioflux. 2021;14(4):1974-89.
4. Ethica SN, Zilda DS, Oedjijono O, Muhtadi M, Patantis G, Darmawati S, et al. Biotechnologically potential genes in a polysaccharide-degrading epibiont of the Indonesian brown algae Hydroclathrus sp. J Genet Eng Biotechnol. 2023;21(1):18. [DOI:10.1186/s43141-023-00461-5] [PMID] []
5. Roy R, Tiwari M, Donelli G, Tiwari V. Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action. Virulence. 2018;9(1):522-54. [DOI:10.1080/21505594.2017.1313372] [PMID]
6. M. Haroon A, M. Daboor S. Nutritional status, antimicrobial and anti-biofilm activity of Potamogeton nodosus Poir. Egyptian Journal of Aquatic Biology and Fisheries. 2019;23(2):81-93. [DOI:10.21608/ejabf.2019.29301]
7. Sa'adah AL. Aktivitas antibiofilm alginat liase Streptomyces olivaceus pada jaringan tikus wistar (Rattus norvegicus) terinfeksi Mycobacterium smegmatis: Magister Study Program of Clinical Laboratory Science Universitas Muhammadiyah Semarang; 2023.
8. Mandasari AA, Wahyuningsih SPA, Darmanto W. Uji Toksisitas akut polisakarida krestin dari ekstrak Coriolus versicolor dengan parameter kerusakan hepatosit, enzim SGPT dan SGOT pada mencit. Jurnal Sain Veteriner. 2015;33(1):69-74.
9. Purba M, Surjanto S, Batubara R. Keamanan teh gaharu (Aquilaria malaccencis Lamk) dari pohon induksi terhadap toksik oral. Wahana Forestra: Jurnal Kehutanan. 2018;13(1):1-11. [DOI:10.31849/forestra.v13i1.1399]
10. Firdausi KM. Uji toksisitas subkronik ekstrak air daun katuk (Sauropus androgynus (L) Merr) terhadap kadar enzim transaminase (AST dan ALT) hepar tikus (Rattus norvegicus) betina: Universitas Islam Negeri Maulana Malik Ibrahim; 2015.
11. Murti FK, Amarwati S, Wijayahadi N. Pengaruh ekstrak daun kersen (Muntingia calabura) terhadap gambaran mikroskopis hepar tikus wistar jantan yang diinduksi etanol dan soft drink. Diponegoro Medical Journal (Jurnal Kedokteran Diponegoro). 2016;5(4):871-83.
12. Murray RK, Granner DK, Victor W. Biokimia Harper: Harper's illustrated biochemistry. In: Soeharsono R, editor. Harper's illustrated biochemistry. Jakarta: BP FKUI; 2014.
13. Shariffah-Muzaimah SA, Idris AS, Madihah AZ, Dzolkhifli O, Kamaruzzaman S, Maizatul-Suriza M. Characterization of Streptomyces spp. isolated from the rhizosphere of oil palm and evaluation of their ability to suppress basal stem rot disease in oil palm seedlings when applied as powder formulations in a glasshouse trial. World J Microbiol Biotechnol. 2017;34(1):15. [DOI:10.1007/s11274-017-2396-1]
14. Puspita Sari D, Hadisusanto S, Istriyati I. Struktur Histologis Hepar, Intestinum, dan Ren Burung Cerek Jawa (Charadrius javanicus Chasen 1938) Dengan Kontaminasi DDT di Delta Sungai Progo Yogyakarta. Biogenesis: Jurnal Ilmiah Biologi. 2014;2(2):126-31. [DOI:10.24252/bio.v2i2.479]
15. Nguyen TNT, Chataway T, Araujo R, Puri M, Franco CMM. Purification and Characterization of a Novel Alginate Lyase from a Marine Streptomyces Species Isolated from Seaweed. Mar Drugs. 2021;19(11). [DOI:10.3390/md19110590] [PMID] []
16. Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE. A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced. Biodiversitas Journal of Biological Diversity. 2019;20(4):1166-72. [DOI:10.13057/biodiv/d200432]
17. Sabbani V, Ramesh A, Shobharani S. Acute Oral Toxicity Studies of Ethanol Leaf Extracts of Derris Scandes & Pulicaria Wightiana in Albino Rats. International Journal Of Pharmacological Research: India. 2015;5(1):1-5.
18. Hasan KMM, Tamanna N, Haque MA. Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed. Food Science and Human Wellness. 2018;7(1):77-82. [DOI:10.1016/j.fshw.2017.12.002]
19. Fitri NMA, Haeni L, Mardliyah E. Pengaruh Pemberian Ekstrak Etanol Kulit Batang Kelor (Moringa oleifera Lam.) sebagai Hepatoprotektor. JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia. 2018;6(2):55-62.
20. López Panqueva RdP. Aspectos morfológicos de la enfermedad hepática inducida por drogas. Revista Colombiana de Gastroenterología. 2014;29(4):449-60. [DOI:10.22516/25007440.445]
21. Rinaldi SF, Mujianto B. Metodologi Penelitian dan Statistik. Bahan Ajar TLM. Jakarta: KEMENKES RI Pusat Pendidikan SDM Kesehatan; 2017.
22. Wolfensohn S, Lloyd M. Handbook of Laboratory Animal Management and Welfare. 3rd ed. USA: Blackwell Publishing; 2013.
23. Abdulwahid SJ, Goh MY, Ebrahimi M, Mohtarrudin N, Hashim ZB. Sub-Acute Oral Toxicity Profiling of the Methanolic Leaf Extract of Clinacanthus Nutans in Male and Female Icr Mice. International Journal of Pharmacy and Pharmaceutical Sciences. 2018;10(12). [DOI:10.22159/ijpps.2018v10i12.28075]
24. Silva AV, Norinder U, Liiv E, Platzack B, Oberg M, Tornqvist E. Associations between clinical signs and pathological findings in toxicity testing. ALTEX. 2021;38(2):198-214. [DOI:10.14573/altex.2003311] [PMID]
25. Kuntz E, Kuntz H-D. Hepatology Textbook and Atlas. Germany: Spinger Medizin Verlag Heidelberg; 2008. [DOI:10.1007/978-3-540-76839-5]
26. Sari HK, Budirahardjo R, Sulistyani E. Kadar Serum Glutamat Piruvat Transaminase (SGPT) pada Tikus Wistar (Rattus norvegicus) Jantan yang Dipapar Stresor Rasa Sakit berupa Electrical Foot Shock selama 28 Hari (The Level of Serum Glutamic Pyrufic Transaminase [SGPT] on a Wistar [Rattus norvegicus]). Pustaka Kesehatan. 2015;3(2):205-11.
27. Rosida A. Pemeriksaan Laboratorium Penyakit Hati. Berkala Kedokteran. 2016;12(1). [DOI:10.20527/jbk.v12i1.364]
28. Parmar K, Singh G, Gupta G, Pathak T, Nayak S. Evaluation of De Ritis ratio in liver-associated diseases. International Journal of Medical Science and Public Health. 2016;5(9). [DOI:10.5455/ijmsph.2016.24122015322]
29. Ndrepepa G. De Ritis ratio and cardiovascular disease: evidence and underlying mechanisms. Journal of Laboratory and Precision Medicine. 2023;8:6-. [DOI:10.21037/jlpm-22-68]
30. Maulina M. ZAT zat yang mempengaruhi histopatologi hepar. Sulawesi: Unimal Press; 2018.
31. Nugrahanti MP, Armalina D, Partiningrum DL, Fulyani F. The effect of ranitidine administration in graded dosage to the degree of liver damage: A study on Wistar rats with acute methanol intoxication. Hum Exp Toxicol. 2021;40(3):497-503. [DOI:10.1177/0960327120954529] [PMID]
32. Stanek M, Rotkiewicz T, Sobotka W, Bogusz J, Otrocka-Domagała I, Rotkiewicz A. The effect of alkaloids present in blue lupine (Lupinus angustifolius) seeds on the growth rate, selected biochemical blood indicators and histopathological changes in the liver of rats. Acta Veterinaria Brno. 2015;84(1):55-62. [DOI:10.2754/avb201585010055]

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