Evaluation of the Subchronic Toxicity of Two Extracts of Tusca Leaves (Acacia aroma) on Rats’ Kidneys, Liver, and Stomach

Taboada, Félix F.; Habib, Natalia C.; Genta, Susana B.

doi:10.32592/IJT.18.2.71

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Volume 18, Issue 2 (May 2024) IJT 2024, 18(2): 71-77 | Back to browse issues page

‎ 10.32592/IJT.18.2.71

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Taboada F F, Habib N C, Genta S B. Evaluation of the Subchronic Toxicity of Two Extracts of Tusca Leaves (Acacia aroma) on Rats’ Kidneys, Liver, and Stomach. IJT 2024; 18 (2) :71-77
URL: http://ijt.arakmu.ac.ir/article-1-1292-en.html

Evaluation of the Subchronic Toxicity of Two Extracts of Tusca Leaves (Acacia aroma) on Rats’ Kidneys, Liver, and Stomach

Félix F. Taboada¹

, Natalia C. Habib¹

, Susana B. Genta ^*

1- Institute of Biology, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán. Superior Institute of Biological Research, National Council of Scientific and Technical Research, Chacabuco 461, San Miguel de Tucumán, Argentina
2- Institute of Biology, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán. Superior Institute of Biological Research, National Council of Scientific and Technical Research, Chacabuco 461, San Miguel de Tucumán, Argentina , susana.genta@fbqf.unt.edu.ar

Abstract: (232 Views)

Background: Acacia aroma (A. aroma), or Tusca, is a South American plant frequently used in medicine. We recently reported the antiulcer effect of two Tusca leaf extracts in rats: a 5% infusion and a 10% hydroalcoholic. This study aimed to investigate the in vitro cytotoxicity and the in vivo oral subchronic toxicity of these two extracts in rats.
Methods: Cytotoxicity was evaluated through cell viability assays using the 50% lethal concentration (LC₅₀) on lung cells, which was calculated for each extract. Oral subchronic toxicity was studied in Wistar rats, and the clinical parameters were determined during a 90-day treatment period. Following the experimental period, biochemical parameters were analyzed, and histopathological analyses of the stomach, liver, and kidneys were performed.
Results: The in vitro cytotoxicity was low in both extracts (LC₅₀=966.78±48.34 µg/ml for the 5% infusion and 562.28±28.11 µg/ml for the 10% hydroalcoholic extract). The in vivo clinical, behavioral, and biochemical parameters, body weight, and food intake had no significant differences (P≥0.05) compared to the control group. There were also no histopathological alterations in the analyzed organs.
Conclusion: The present study contributes to expanding the knowledge on the safety of the two extracts derived from A. aroma (5% infusion and 10% hydroalcoholic). Therefore, the extracts can possibly be used in the treatment or prevention of gastric diseases in future studies.

Keywords: Acacia aroma, Cytotoxic activity, Preclinical study, Subchronic toxicity, Tusca leaf extracts

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Type of Study: Research | Subject: General

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