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Volume 18, Issue 2 (May 2024)
IJT 2024, 18(2): 61-70 |
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Ethics code: 01/21
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Fernandes de Lima Neto M, Igo Teixeira de Assis E, Venância Nunes Azevedo A, Raiane Feitosa Melo Paulino L, Aragão Matos Donato M, Alves Peixoto C, et al . Extract of Actaea racemosa Protects Mice Ovarian Follicles Against Doxorubicin-induced Toxicity. IJT 2024; 18 (2) :61-70
URL: http://ijt.arakmu.ac.ir/article-1-1333-en.html
URL: http://ijt.arakmu.ac.ir/article-1-1333-en.html
Miguel Fernandes de Lima Neto1 
, Ernando Igo Teixeira de Assis1 , Antônia Venância Nunes Azevedo1 , Laís Raiane Feitosa Melo Paulino1 , Mariana Aragão Matos Donato2 , Christina Alves Peixoto2 , Alane Pains Oliveira do Monte3 , Maria Helena Tavares de Matos3 , Alana Nogueira Godinho4 , Jordânia Marques de Oliveira Freire4 , Ricássio De Sousa Barberino3 , Anderson Weiny Barbalho Silva1 , José Roberto Viana Silva * 5
, Ernando Igo Teixeira de Assis1 , Antônia Venância Nunes Azevedo1 , Laís Raiane Feitosa Melo Paulino1 , Mariana Aragão Matos Donato2 , Christina Alves Peixoto2 , Alane Pains Oliveira do Monte3 , Maria Helena Tavares de Matos3 , Alana Nogueira Godinho4 , Jordânia Marques de Oliveira Freire4 , Ricássio De Sousa Barberino3 , Anderson Weiny Barbalho Silva1 , José Roberto Viana Silva * 5
1- Laboratory of Biotechnology and Physiology of Reproduction–LABIREP, Federal University of Ceara, Sobral, CE, Brazil
2- Laboratory of Ultrastructure, CNPqAM/FIOCRUZ, Federal University of Pernambuco Recife, PE, Brazil
3- Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
4- Nucleus of Research in Animal Experimentation–NUPEX, Federal University of Ceara, Sobral, CE, Brazil
5- Laboratory of Biotechnology and Physiology of Reproduction–LABIREP, Federal University of Ceara, Sobral, CE, Brazil ,jrvsilva@ufc.br
2- Laboratory of Ultrastructure, CNPqAM/FIOCRUZ, Federal University of Pernambuco Recife, PE, Brazil
3- Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
4- Nucleus of Research in Animal Experimentation–NUPEX, Federal University of Ceara, Sobral, CE, Brazil
5- Laboratory of Biotechnology and Physiology of Reproduction–LABIREP, Federal University of Ceara, Sobral, CE, Brazil ,
Abstract: (284 Views)
Background: Given the cytotoxicity of chemotherapy drugs used in cancer treatment, there is a need to develop alternative agents to protect female fertility. This study investigated the effect of Actaea racemosa (A. racemosa) extract on mice ovarian cells and the damage caused by doxorubicin (DOX) to the mice ovaries.
Methods: We evaluated the effects of A. racemosa extract on mice ovaries (n=42) after DOX treatment. The mice were pre-treated with saline solution (controls) or with 0.5 or 5 mg/kg A. Racemosa extract. Afterward, during a period of 10 days, they were treated daily with one of the six protocols: (i) saline solution (control), (ii) 10 mg/kg DOX, (iii) 0.5 mg/kg A. racemosa extract, (iv) both DOX and 5 mg/kg A. racemosa extract, (v) A. racemosa extract (5 mg/kg), and (vi) both DOX and 0.5 mg/kg A. racemosa extract. At the end of these treatments, the ovaries were fixed for histopathological examinations. Ovarian follicular morphology, stromal cell density, collagen fibers, and TNF-α expression were evaluated. Some ovaries were fixed for transmission electron microscopy or stored at -80oC to study the mRNA expression for Caspase-3 and TNF-α.
Results: The Mice treated with A. racemosa extract had reduced follicular degeneration and cell death after exposure to DOX. Ovaries of mice treated with 0.5 mg/kg A. racemosa extract had granulosa cells and oocytes with preserved ultrastructure, decreased immunostaining for TNF-α, and reduced Caspase-3 mRNA.
Conclusion: The A. racemosa extract supported follicular survival and protected the ovarian follicles and stromal cells against DOX-induced cytotoxicity.
Methods: We evaluated the effects of A. racemosa extract on mice ovaries (n=42) after DOX treatment. The mice were pre-treated with saline solution (controls) or with 0.5 or 5 mg/kg A. Racemosa extract. Afterward, during a period of 10 days, they were treated daily with one of the six protocols: (i) saline solution (control), (ii) 10 mg/kg DOX, (iii) 0.5 mg/kg A. racemosa extract, (iv) both DOX and 5 mg/kg A. racemosa extract, (v) A. racemosa extract (5 mg/kg), and (vi) both DOX and 0.5 mg/kg A. racemosa extract. At the end of these treatments, the ovaries were fixed for histopathological examinations. Ovarian follicular morphology, stromal cell density, collagen fibers, and TNF-α expression were evaluated. Some ovaries were fixed for transmission electron microscopy or stored at -80oC to study the mRNA expression for Caspase-3 and TNF-α.
Results: The Mice treated with A. racemosa extract had reduced follicular degeneration and cell death after exposure to DOX. Ovaries of mice treated with 0.5 mg/kg A. racemosa extract had granulosa cells and oocytes with preserved ultrastructure, decreased immunostaining for TNF-α, and reduced Caspase-3 mRNA.
Conclusion: The A. racemosa extract supported follicular survival and protected the ovarian follicles and stromal cells against DOX-induced cytotoxicity.
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