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Volume 1, Issue 2 (Summer 2007)
IJT 2007, 1(2): 3-3 |
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Raees Ahmad K. Teratological Effects of Chlorpyrifos in Mice. IJT 2007; 1 (2) :3-3
URL: http://ijt.arakmu.ac.ir/article-1-27-en.html
URL: http://ijt.arakmu.ac.ir/article-1-27-en.html
1- Department of Biological Sciences, Assistant Professor of Zoology University of Sargodha, Sargodha. Pakistan , khawajaraees@hotmail.com
Abstract: (12851 Views)
Introduction: Chlorpyrifos (CPF), an organophosphate insecticide, was evaluated for potential teratogenic effects in mice. A single oral dose of insecticide (i.e. 72, 36 18 or 9 mg/kg) was given to the pregnant females on gestation day6 (GD6). These doses correspond 50, 25, 12.5 and 6.25% to the calculated LD50 value (144 mg/kg) for the pregnant mice. No signs of overt toxicity were observed at any of these doses.
Method and Materials: Fetuses recovered from 18, 36 and 72 mg/kg groups showed a variety of morphological defects i.e. microcephaly, hydrocephaly, agnathia, anophthalmia, enlarged meningio-encepahlocoele, Spina-bifida, meromelia, micromelia, drooping wrists, rudimentary or kinky tails, round back, twisted spine, tortuous-limbs, flipper feet and sacral hygromas. Fetuses recovered from vehicle treated and 9mg/kg groups were apparently normal.
Results: Statistical analysis of the data based on single factor ANOVA and Duncan’s Multiple Range Test have shown a dose dependent decrease in litter size (P < 0.001) along with averaged per litter: fetal weight (P < 0.01) and crown rump length (P < 0.05). The extent of ossification in embryonic skeleton decreased progressively with increased maternal CPF exposure.
Conclusion: Based on these findings we conclude that, if treated on GD6, a single oral dose of 18 mg/kg CPF (12.5% of LD50) may lead to several craniofacial and skeletal anomalies in developing mice embryos, whereas its fetotoxic role is fairly dose dependent embryos.
Method and Materials: Fetuses recovered from 18, 36 and 72 mg/kg groups showed a variety of morphological defects i.e. microcephaly, hydrocephaly, agnathia, anophthalmia, enlarged meningio-encepahlocoele, Spina-bifida, meromelia, micromelia, drooping wrists, rudimentary or kinky tails, round back, twisted spine, tortuous-limbs, flipper feet and sacral hygromas. Fetuses recovered from vehicle treated and 9mg/kg groups were apparently normal.
Results: Statistical analysis of the data based on single factor ANOVA and Duncan’s Multiple Range Test have shown a dose dependent decrease in litter size (P < 0.001) along with averaged per litter: fetal weight (P < 0.01) and crown rump length (P < 0.05). The extent of ossification in embryonic skeleton decreased progressively with increased maternal CPF exposure.
Conclusion: Based on these findings we conclude that, if treated on GD6, a single oral dose of 18 mg/kg CPF (12.5% of LD50) may lead to several craniofacial and skeletal anomalies in developing mice embryos, whereas its fetotoxic role is fairly dose dependent embryos.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
