Write your message
Volume 12, Issue 2 (March-April 2018)                   IJT 2018, 12(2): 37-43 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tasneem S, Yasmeen R. Induction of Micronuclei and Erythrocytic Nuclear Abnormalities in Peripheral Blood of Fish Cyprinus carpio on Exposure to Karanjin. IJT. 2018; 12 (2) :37-43
URL: http://ijt.arakmu.ac.ir/article-1-635-en.html
1- Department of Zoology, University College of Science, Osmania University, Hyderabad, India. , shoeiba.tas@gmail.com
2- Department of Zoology, University College of Science, Osmania University, Hyderabad, India.
Abstract:   (4498 Views)
Background: We have come across the plant secondary metabolites having pesticidal properties being used in the form of pesticides. In this study, we used one of newly available and plant metabolite used as pesticide in wide agricultural fields – Karanjin, obtained from seeds of plant Pongamia pinnata.
Methods: The study was conducted during the month of March 2016 at the Department of Zoology, Osmania University. The fish common carp- Cyprinus carpio was exposed to sub-lethal concentration of karanjin i.e., 1/10th of 96 h LC50 value (0.28 ppm) for a period of 21 d. Moreover, at 24 h, 7 d, 14 d and 21 d, the peripheral blood of both control and exposed group fishes were studied for the presences of micronuclei and other nuclear abnormalities.
Results: The micronuclei were completely absent, i.e., were not seen during the sub-lethal exposure period. There were seen nuclear abnormalities such as blebbed nuclei (BN), notched nuclei (NN), differently shaped nuclei (DSN), pear-shaped nuclei (PSN), circular nuclei (CN), lobed nuclei (LN) and Karyolysed nuclei (KN). The control group showed few nuclear abnormalities.
Conclusion: During the sub-lethal exposure, as the days of exposure increased, the types of aberrations and their number also increased.
Full-Text [PDF 781 kb]   (935 Downloads)    
Type of Study: Research | Subject: Special

1. Marchezan E, Reimche G, Avila L. Toxicological and metabolical parameters of the teleost fish (Leporinus obtusidens) in response to commercial herbicides containing clomazone and propanil. Pest Biochem Physiol 2009;95:57-62. [DOI:10.1016/j.pestbp.2009.06.006]
2. Agah H, Leermakers M, Elskens M, Fatemi SMR,Baeyens W. Accumulation of trace metals in the muscles and liver tissues of five fish species from the Persian Gulf. Environ Monit Assess 2009; 157: 499-514. [DOI:10.1007/s10661-008-0551-8]
3. Arkhipchuk V, Garanko N. Using the nucleolar biomarker and the micronucleus test on in vivo fish fin cells. Ecotoxicol environ saf 2005;62(1):42-52. [DOI:10.1016/j.ecoenv.2005.01.001]
4. Ateeq B, Ali MN, Ahmad W. Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2, 4-dichlorophenoxyacetic acid and butachlor. Mutation Res 2002;518(2):135-44. [DOI:10.1016/S1383-5718(02)00075-X]
5. Al-Sabti K, Metcalfe CD. Fish micronuclei for assessing genotoxicity in water. Genet Toxicol 1995; 343:121-35. [DOI:10.1016/0165-1218(95)90078-0]
6. Ferraro MVM, Fenocchio AS, Mantovani MS, Ribeiro CO, Cestari MM. Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. Malabaricus as evaluated using the comet assay and piscine micronucleus and chromosome aberration tests. Genet Mol Biol 2004; 27:103-7. [DOI:10.1590/S1415-47572004000100017]
7. Çavas T, Garanko NN, Arkhipchuk VV. Induction of micronuclei and binuclei in blood, gill and liver cells of fishes sub-chronically exposed to cadmium chlorid and copper sulphate. Food Chem Toxicol 2005; 43:569-74. [DOI:10.1016/j.fct.2004.12.014]
8. Çavaş T, Ergene-Gözükara S. Micronuclei, nuclear lesions and interphase silver-stained nucleolar organizer regions (AgNORs) as cyto-genotoxicity indicators in Oreochromis niloticus exposed to textile mill effluent. Mutat Res 2003;538(1):81-91. [DOI:10.1016/S1383-5718(03)00091-3]
9. Ayllón F, Garcia-Vazquez E. Induction of micronuclei and other nuclear abnormalidades in European minnow Phoxinusphoxinus and mollie Poecilialatipinna: An assessment of the fish micro nucleus test. Mutat Res 2000; 467:177-86. [DOI:10.1016/S1383-5718(00)00033-4]
10. Franco-Bernardes MF, Maschio LR, De Azeredo-Oliveira MTV, De alimeida EA. Biochemical and genotoxic effects of a commercial formulation of the herbicide tebuthiuron in Oreochromisniloticus of different sizes. Ecotoxicol Environ Cont 2014; 9(10), 59-67. [DOI:10.5132/eec.2014.01.008]
11. Çavas T, Ergene-Gözükara S. Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluents. Aquat Toxicol 2005; 74:264-71. [DOI:10.1016/j.aquatox.2005.06.001]
12. Araújo CVM, Cohin-de-Pinho SJ, Santos JS, Delgado F, Santana LCS, Chastinet CBA, Silva EM. In situ and laboratory bioassays using Poeciliareticulata Peters, 1859 in the biomonitoring of an acidic lake at Camaçari, BA, Brazil. Chemosphere 2006; 65:599-603. [DOI:10.1016/j.chemosphere.2006.02.006]
13. Hayashi M, Ueda T, Uyeno K, Wada K, Kinae N, Saotme K, Tanaka N, Takai A, Sasaki YF, Asano N. Development of genotoxicity assay systems that use aquatic organisms. Mutat Res 1998; 399:125-33. [DOI:10.1016/S0027-5107(97)00251-0]
14. Teles M, Pacheco M, Santos MA.Anguilla anguilla L. Liver ethoxyresorufin O-deethylation, glutathione S-tranferase, erythrocytic nuclear abnormalities, and endocrine responses to naphthalene and β-naphthoflavone. Ecotoxicol Environ Saf 2003; 55:98-107. [DOI:10.1016/S0147-6513(02)00134-3]
15. Buschini A, Martino A, Gustavino B, Monfrinotti M, Poli P, Rossi C, Santoro M, Dörr AJ MandRizzoni M. Comet assay and micronucleus test in circulation erythrocytes of Cyprinus carpios pecimes exposed in situ to lake waters treated with disinfectants for potabilization. Mutat Res 2004; 557:119-29. [DOI:10.1016/j.mrgentox.2003.10.008]
16. Kayhanian M, Suverkropp C, Ruby A,Tsay K. Characterization and prediction of highway runoff constituent event mean concentration. J Environ Manage 2007; 85:279-95. [DOI:10.1016/j.jenvman.2006.09.024]
17. Davis AP, Shokouhian M, Ni S. Loading estimates of lead, copper, cadmiun, and zinc in urban runoff from specific sources. Chemosphere 2000; 44:997-1009. [DOI:10.1016/S0045-6535(00)00561-0]
18. Maria VL, Gravato C, Correria AC, Santos MA. Biotransformation and genotoxicity responses to PHAs in two teleost species. Fresenius Environ Bull 2002; 11: 609-15.
19. Gravato C, Santos MA. Juvenile sea bass liver P450, EROD induction, and erythrocytic genotoxic responses to PAH and PAH-like compounds. Ecotoxicol Environ Saf 2002; 51:115-27. [DOI:10.1006/eesa.2001.2133]
20. Gravato C, Santos M. Genotoxicity biomarkers' association with B (a) P biotransformation in Dicentrarchus labrax L. Ecotoxicol Environ Saf 2003;55(3):352-8. [DOI:10.1016/S0147-6513(02)00070-2]
21. Pacheco M, Santos MA. Biotransformation, endocrine, and genetic responses of (Anguilla anguilla L) to petroleum distillate products and environmentally contaminated waters. Ecotoxicol Environ Saf 2001; 49: 64-75. [DOI:10.1006/eesa.2000.2025]
22. Pacheco M, Santos MA. Induction of liver EROD activity and erythrocytic nuclear abnormalities by cyclophosphamide and PAHs in Anguilla anguilla L. Ecotoxicol Environ Saf 1998; 40: 71-6. [DOI:10.1006/eesa.1998.1644]
23. Ayllon F, Garcia-Vazquez E. Micronuclei and other nuclear lesions as genotoxicity indicators in rainbow trout Oncorhynchus mykiss. Ecotoxicol Environ Saf 2001; 49:221-5. [DOI:10.1006/eesa.2001.2065]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2021 CC BY-NC 4.0 | Iranian Journal of Toxicology

Designed & Developed by : Yektaweb