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Volume 13, Issue 1 (January 2019)
IJT 2019, 13(1): 13-18 |
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Hajari Z, Fathi B, Saadatfar Z, Zare A. Antagonistic Effects of Suramin Against the Venom of Snake, Echis Carinatus, on the Circulatory System of Developing Chicken Embryos. IJT 2019; 13 (1) :13-18
URL: http://ijt.arakmu.ac.ir/article-1-722-en.html
URL: http://ijt.arakmu.ac.ir/article-1-722-en.html
1- Department of Basic sciences (Pharmacology), School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
2- Department of Basic sciences (Pharmacology), School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. , b-fathi@ferdowsi.um.ac.ir
3- PhD of Biochemistry and Toxicology, Razi Vaccine and Serum Research Institute, Tehran, Iran.
2- Department of Basic sciences (Pharmacology), School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. , b-fathi@ferdowsi.um.ac.ir
3- PhD of Biochemistry and Toxicology, Razi Vaccine and Serum Research Institute, Tehran, Iran.
Abstract: (3168 Views)
Background: The snake, Echis carinatus, one of the most venomous snakes in Asia, possesses a deadly hemotoxic venom. It has been reported that suramin, an anti-trypansomiasis drug, can inhibit the toxic effects of some snake venoms. This study was conducted to evaluate the possible antagonistic effects of suramin against the hemorrhagic activity of the venom from an Iranian snake, Echis carinatus, in developing chicken embryos.
Methods: One day old fertile eggs (n=250) were incubated for six days at 37ºC and 60% humidity. Paper discs (5 mm diameter) containing different concentrations of E. carinatus venom (5, 10, 20 & 30 μg) were placed on the chorioallantoic membrane over the major bilateral vein and were left in place until hemorrage occurred and the embryos died.
Results: We found the standard hemorrhagic dose (SHD) of E. carinatus venom to be (5 μg/disc). Various concentrations (5, 10 & 20 μg) of suramin were tested against SHD of E. carinatus venom in different protocols. Control experiments were performed with the buffered saline solution, venom and suramin individually. The results demonstrated that suramin at 5, 10 and 20 μg significantly (P<0.05) delayed time to death (717, 521 & 208 min) of embryos poisoned with E. carinatus venom in a dose-dependent manner.
Conclusion: Suramin exerted protective and inhibitory effects against the deadly E. carinatus venom, and therefore, may potentially offer future therapeutic applications against poisoning with E. carinatus venom.
Methods: One day old fertile eggs (n=250) were incubated for six days at 37ºC and 60% humidity. Paper discs (5 mm diameter) containing different concentrations of E. carinatus venom (5, 10, 20 & 30 μg) were placed on the chorioallantoic membrane over the major bilateral vein and were left in place until hemorrage occurred and the embryos died.
Results: We found the standard hemorrhagic dose (SHD) of E. carinatus venom to be (5 μg/disc). Various concentrations (5, 10 & 20 μg) of suramin were tested against SHD of E. carinatus venom in different protocols. Control experiments were performed with the buffered saline solution, venom and suramin individually. The results demonstrated that suramin at 5, 10 and 20 μg significantly (P<0.05) delayed time to death (717, 521 & 208 min) of embryos poisoned with E. carinatus venom in a dose-dependent manner.
Conclusion: Suramin exerted protective and inhibitory effects against the deadly E. carinatus venom, and therefore, may potentially offer future therapeutic applications against poisoning with E. carinatus venom.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
