Abstract
Nowadays, due to the problems of increasing microbial resistance, scientists are trying to find the most effective and safest way to overcome them. Colloidal nanoparticles of Ag (cAgNPs) and Tio2 (cTio2NPs), can be effective against many microorganisms. Therefore, the purpose of the current study was to investigate the antibacterial properties and cytotoxicity assessment of cAgNPs and cTio2NPs, against Shigella dysenteriae ATCC 13313 and Staphylococcus aureus ATCC 25922. The UV absorption spectra of cAgNPs and cTiO2 NPs showed the highest absorption at 313 and 206 nm, respectively. The average size of nanoparticles of Ag and TiO2 was 18.2 ± 0.1 and 20.6 ± 0.2. The morphology of nanoparticles indicated spherical shapes with smooth surfaces. XRD pattern of cAgNPs and cTio2NPs showed sharp characteristic peaks at about 2𝜃 = 24∘ and about 2𝜃 = 26∘, related to the crystal formation of both Ag and Tio2. It was observed that MIC and MBC of cAgNPs and cTio2NPs were similar (12.5 µg/ml) against S. aureus while MIC of cAgNPs and cTio2NPs against S. dysenteria founded 12.5 and 25 µg/ml. In addition, MBC of cAgNPs and Tio2NPs for S. dysenteriae were 25 and 50 µg/ml. The cAgNPs and cTio2NPs indicated good antibacterial properties and had low toxicity against the Huo2 fibroblast cell lines.