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Volume 17, Issue 4 (In Press 2023)
IJT 2023, 17(4): 0-0 |
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Vali A, Soleimani Badie M, Dastan D, Moradkhani S. Screening Cytotoxicity of 10 Plants from Lamiaceae Family on MCF-7 Breast Cancer Cell Line and Investigating the Selectivity Indices for Fractions and Essential Oil of the Most Effective Plant. IJT 2023; 17 (4)
URL: http://ijt.arakmu.ac.ir/article-1-1251-en.html
URL: http://ijt.arakmu.ac.ir/article-1-1251-en.html
1- Department f Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Product Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
2- Department f Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
3- Department f Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Product Research Center, Hamadan University of Medical Sciences, Hamadan, Iran , shirin.moradkhani@yahoo.com
2- Department f Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
3- Department f Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Product Research Center, Hamadan University of Medical Sciences, Hamadan, Iran , shirin.moradkhani@yahoo.com
Abstract: (56 Views)
Background:
Breast cancer is considered to be the most common cancer in women. The plants of the Lamiaceae family have been found to exert in-vitro anti-proliferative activity on breast cancer cells. This research aims to evaluate the cytotoxic activity of the ten species from the Lamiaceae family on the breast cancer cell line, as well as study the selective indices of fractions and essential oil of the most effective plant.
Material and methods:
The plant species were harvested, dried and authenticated. Then, the hydroalcoholic extract (HAE) of each plant was investigated for cytotoxicity on the MCF-7 breast cancer cell line by the MTT assay.
The HAE of Nepeta crispa was fractionated using n-hexane, Chloroform and Ethyl Acetate. The essential oil of Nepeta crispa was isolated by hydro-distillation. Then, the fractions and essential oil (EO) of Nepeta crispa were investigated for in vitro cytotoxic effects against both MCF-7 and HEK293 cell lines.
Results:
The HAE of Nepeta crispa exhibited significant cytotoxic activity on MCF-7 (IC50 = 59 ± 3.4 μg/mL) among other extracts.
The n-hexane fraction of Nepeta crispa displayed the highest cytotoxicity (IC50 = 65.47± 4.3 μg/ml) among fractions.
The essential oil of Nepeta crispa exhibited concentration-dependent growth inhibition of cancer cells, and it possessed the most inhibitory effect against MCF-7 cells (IC50 = 18.15 ± 2.7 μg/mL) with a selectivity index of 9.69 for MCF-7 cells.
Conclusion:
Based on the results, the n-hexane fraction and EO of Nepeta crispa may be the potential sources of biologically active components to develop novel drugs for breast cancer treatment.
Breast cancer is considered to be the most common cancer in women. The plants of the Lamiaceae family have been found to exert in-vitro anti-proliferative activity on breast cancer cells. This research aims to evaluate the cytotoxic activity of the ten species from the Lamiaceae family on the breast cancer cell line, as well as study the selective indices of fractions and essential oil of the most effective plant.
Material and methods:
The plant species were harvested, dried and authenticated. Then, the hydroalcoholic extract (HAE) of each plant was investigated for cytotoxicity on the MCF-7 breast cancer cell line by the MTT assay.
The HAE of Nepeta crispa was fractionated using n-hexane, Chloroform and Ethyl Acetate. The essential oil of Nepeta crispa was isolated by hydro-distillation. Then, the fractions and essential oil (EO) of Nepeta crispa were investigated for in vitro cytotoxic effects against both MCF-7 and HEK293 cell lines.
Results:
The HAE of Nepeta crispa exhibited significant cytotoxic activity on MCF-7 (IC50 = 59 ± 3.4 μg/mL) among other extracts.
The n-hexane fraction of Nepeta crispa displayed the highest cytotoxicity (IC50 = 65.47± 4.3 μg/ml) among fractions.
The essential oil of Nepeta crispa exhibited concentration-dependent growth inhibition of cancer cells, and it possessed the most inhibitory effect against MCF-7 cells (IC50 = 18.15 ± 2.7 μg/mL) with a selectivity index of 9.69 for MCF-7 cells.
Conclusion:
Based on the results, the n-hexane fraction and EO of Nepeta crispa may be the potential sources of biologically active components to develop novel drugs for breast cancer treatment.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
