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Volume 18, Issue 1 (January 2024)                   IJT 2024, 18(1): 39-44 | Back to browse issues page

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Asoudeh-Fard A, Najafipour R, Salehi M, Mahmoudi M, Salahshourifar I, Eghdami A, et al . Apoptotic Effect of Phycocyanin on HT-29 Colon Cancer through Activation of Caspase Enzymes and P53 Cell Signaling Pathway. IJT 2024; 18 (1) :39-44
URL: http://ijt.arakmu.ac.ir/article-1-1253-en.html
Apoptotic Effect of Phycocyanin on HT-29 Colon Cancer through Activation of Caspase Enzymes and P53 Cell Signaling Pathway
Abbas Asoudeh-Fard1 , Reza Najafipour2 , Mitra Salehi3 , Mahsa Mahmoudi4 , Iman Salahshourifar5 , Anoosh Eghdami6 , Asghar Parsaei7 , Hossein Piri * 8
1- INSERM U1148, Laboratory for Vascular Translation Science (LVTS), Cardiovascular Bioengineering, University Sorbonne Paris North, Paris, France.
2- Genetics Research Center, the University of Social Welfare and Rehabilitation Science, Tehran, Iran.
3- Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
4- Faculty of Convergent Sciences and Technologies Islamic Azad University of Science and Research Tehran Iran.
5- Assistant professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
6- Department of Biochemistry, School of Medicine, Saveh Branch, Islamic Azad University, Saveh, Iran.
7- Rayan Novin Pajoohan Pras, Biotechnology Company, Biotechnology Incubator, Shiraz University of Medical Sciences, Shiraz, Iran.
8- Cellular and Molecular Research Center, Research Institute for prevention of Non-Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran , hosseinpiry@gmail.com
Abstract:   (383 Views)
Background: C-phycocyanin, a biliprotein from Spirulina platensis, is a future candidate for cancer management. This agent is originated from edible blue-green algae, and numerous in vivo and in vitro research have reported on its anti-cancer properties. The effects of C-phycocyanin have been investigated on caspases 3, 8, 9, and p53 pathways in the human colorectal adenocarcinoma cell line (HT-29) and human umbilical vein endothelial cells (HUVECs).
Methods: In the current study, we investigated the effect of C-phycocyanin on caspase 3, 8, 9, and p53-mediated apoptosis pathways in two cell lines (HT-29 & HUVEC), using quantitative real-time PCR and flow cytometry. The cytotoxicity of phycocyanin on HT-29 cells was compared with HUVEC normal cells via colorimetric assays.
Results: Based on our findings at molecular level, the expression of caspases 3, 8, 9, and p53 genes were increased in colorectal cancer cells treated with C-phycocyanin.The results were confirmed by an increase in the number of colorectal cancer cells in the early and late stages of apoptosis as compared to the control, untreated cells. In addition, the results of colorimetric assay showed that C-phycocyanin has no cytotoxic effects on normal HUVECs cells.
Conclusion: Based on our experimental data, it is evident that C-phycocyanin has measurable effects on cell apoptosis. Since tumorigenesis is halted by apoptosis, C-phycocyanin can be a hopeful candidate for the treatment of human colorectal cancer in the future.
Keywords: Apoptosis, Caspases, Colorectal Cancer, Human Umbilical Vein Endothelial Cells, Phycocyanin, Tumor Suppressor Protein p53
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Type of Study: Research | Subject: Special
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