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Volume 18, Issue 1 (January 2024)
IJT 2024, 18(1): 45-51 |
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Jimat R T, Ethica S N, Rahmani N, Yulianti S E, Rachmayati R, Nuryati N, et al . Alginate Lyase from Streptomyces olivaceus is a Safe and Effective Antibiofilm in Male Wistar Rats (Rattus norvegicus). IJT 2024; 18 (1) :45-51
URL: http://ijt.arakmu.ac.ir/article-1-1274-en.html
URL: http://ijt.arakmu.ac.ir/article-1-1274-en.html
Resi Tondho Jimat1 
, Stalis Norma Ethica1 , Nanik Rahmani2 , Siti Eka Yulianti2 , Rike Rachmayati2 , Nuryati Nuryati2 , Akhirta Atikana2 , Shanti Ratnakomala3 , Puspita Lisdiyanti3 , Yopi Yopi4 , Dewi Seswita Zilda5 , Maya Dian Rakhmawatie * 6
, Stalis Norma Ethica1 , Nanik Rahmani2 , Siti Eka Yulianti2 , Rike Rachmayati2 , Nuryati Nuryati2 , Akhirta Atikana2 , Shanti Ratnakomala3 , Puspita Lisdiyanti3 , Yopi Yopi4 , Dewi Seswita Zilda5 , Maya Dian Rakhmawatie * 6
1- Magister Study Program of Clinical Laboratory Science, Postgraduate Program, Universitas Muhammadiyah Semarang
2- Research Center for applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency
3- Research Center for Biosystematics and Evolution, Organization Research of Life Sciences and Environment, National Research and Innovation Agency
4- Deputy for Regional Research and Innovation, National Research and Innovation Agency
5- Research Center for Deep Sea, Earth Sciences and Maritime Research Organization, National Research and Innovation Agency
6- Department of Biomedical Sciences, Faculty of Medicine, Universitas Muhammadiyah Semarang , mayadianr@unimus.ac.id
2- Research Center for applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency
3- Research Center for Biosystematics and Evolution, Organization Research of Life Sciences and Environment, National Research and Innovation Agency
4- Deputy for Regional Research and Innovation, National Research and Innovation Agency
5- Research Center for Deep Sea, Earth Sciences and Maritime Research Organization, National Research and Innovation Agency
6- Department of Biomedical Sciences, Faculty of Medicine, Universitas Muhammadiyah Semarang , mayadianr@unimus.ac.id
Abstract: (396 Views)
Background: Alginate lyase is known to have antibiofilm activity. Toxicity tests for the alginate lyase produced by Streptomyces olivaceus from Serena Kecil Island, North Sulawesi, Indonesia, have never been reported. This research conducted an in vivo toxicity study of the alginate lyase, using male Wistar rats for its development as an oral anti-biofilm agent.
Methods: Twenty male Wistar rats (Rattus norvegicus) were divided equally into four groups of: technical control (TC), which was only given food and drink; negative control (NC), given phosphate buffer saline 1x; experimental group (E1), given alginate lyase at a dose of 0.63 g/kg BW, and (E2), given alginate lyase at a dose of 20.85 g/kg BW. The toxicity tests were carried out for 7 days by observing the following parameters: changes in the body weight and behavior; changes in AST and ALT enzyme levels; and evaluation of macroscopic and microscopic damages to the liver.
Results: After 7 days of treatment, rats in the NC, E1, and E2 groups experienced insignificant weight losses. They also had normal behavior and did not show signs of toxicity or death. The AST and ALT levels in rats, given alginate lyase (E1 and E2) decreased significantly but were still within the normal range. Alginate lyase also caused an adaptive stress response and degeneration in the liver cells.
Conclusion: Alginate lyase at 0.63 g/kg BW and 20.85 g/kg BW were considered safe in the rats and had the prospect of being developed into an anti-biofilm agent.
Methods: Twenty male Wistar rats (Rattus norvegicus) were divided equally into four groups of: technical control (TC), which was only given food and drink; negative control (NC), given phosphate buffer saline 1x; experimental group (E1), given alginate lyase at a dose of 0.63 g/kg BW, and (E2), given alginate lyase at a dose of 20.85 g/kg BW. The toxicity tests were carried out for 7 days by observing the following parameters: changes in the body weight and behavior; changes in AST and ALT enzyme levels; and evaluation of macroscopic and microscopic damages to the liver.
Results: After 7 days of treatment, rats in the NC, E1, and E2 groups experienced insignificant weight losses. They also had normal behavior and did not show signs of toxicity or death. The AST and ALT levels in rats, given alginate lyase (E1 and E2) decreased significantly but were still within the normal range. Alginate lyase also caused an adaptive stress response and degeneration in the liver cells.
Conclusion: Alginate lyase at 0.63 g/kg BW and 20.85 g/kg BW were considered safe in the rats and had the prospect of being developed into an anti-biofilm agent.
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Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
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