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Volume 18, Issue 2 (May 2024)
IJT 2024, 18(2): 106-112 |
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Ethics code: YDU/2020/83-1163
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Muhammed D S H, Saygi S. Urinary Bisphenol-A Identification in an Iraqi Population. IJT 2024; 18 (2) :106-112
URL: http://ijt.arakmu.ac.ir/article-1-1290-en.html
URL: http://ijt.arakmu.ac.ir/article-1-1290-en.html
1- Department of Toxicology, Faculty of Pharmacy, Near East University, Nicosia, North Cyprus , dastansalim@yahoo.com
2- Professor, Department of Toxicology, Faculty of Pharmacy, Near East University, Nicosia, North Cyprus
2- Professor, Department of Toxicology, Faculty of Pharmacy, Near East University, Nicosia, North Cyprus
Abstract: (827 Views)
Background: Investigation into environmental chemicals and their impact on human health is a topic of great interest. It is essential to understand the relationship between the quantity of chemical substances involved and the prevalence of particular diseases. Bisphenol-A (BPA) is a disrupting agent to human endocrine health. Human exposure to BPA is on the rise due to the widespread use of this agent, particularly in manufacturing industries. This study aimed to investigate the concentrations of BPA in the Iraqi population.
Methods: This study aimed to determine the amount of BPA in the urine samples of 100 adults residing in the three cities of Diyalah, Kirkuk, and Solaymania in northeastern Iraq. After the subjects’ urine samples were collected, the overall, free, and conjugated BPA contents of the samples were measured using high-performance liquid chromatography and mass spectroscopy (HPLC/MS). The data were adjusted based on the precalculated creatinine levels in the subjects.
Results: Urine samples were collected from 100 individuals residing in three different cities in Iraq, comprising 47 men and 53 women. The concentrations of BPA in all participants’ urine samples ranged from greater than the limit of quantification to 80.24 µg/g creatinine, with a calculated geometric mean of 4.17 µg/g creatinine.
Conclusion: The findings of this preliminary study indicated that the Iraqi population’s level of BPA exposure falls within an acceptable range. To provide a more accurate estimate of BPA exposure nationwide, further investigations on larger sample sizes are required across a wider range of age and gender distributions.
Methods: This study aimed to determine the amount of BPA in the urine samples of 100 adults residing in the three cities of Diyalah, Kirkuk, and Solaymania in northeastern Iraq. After the subjects’ urine samples were collected, the overall, free, and conjugated BPA contents of the samples were measured using high-performance liquid chromatography and mass spectroscopy (HPLC/MS). The data were adjusted based on the precalculated creatinine levels in the subjects.
Results: Urine samples were collected from 100 individuals residing in three different cities in Iraq, comprising 47 men and 53 women. The concentrations of BPA in all participants’ urine samples ranged from greater than the limit of quantification to 80.24 µg/g creatinine, with a calculated geometric mean of 4.17 µg/g creatinine.
Conclusion: The findings of this preliminary study indicated that the Iraqi population’s level of BPA exposure falls within an acceptable range. To provide a more accurate estimate of BPA exposure nationwide, further investigations on larger sample sizes are required across a wider range of age and gender distributions.
References
1. Kafle A, Mohapatra S, Panigrahi S, Ganguly S. Enviromental Toxicology. In Ashok G. Matani, Dr. Shamal K. Doifode. Environment and Energy: Latest Progress. Delhi: International Research Publication House. 2018; 251-69. [Link]
2. Fisher MR. Environmental biology. Revised ed. Oregon: creative commons. 2019. [Link]
3. Dianin AP. Condensation of ketones with phenols. Zhurnal Russkogo Fiziko-Khimicheskogo Obshchestva. J. Russ. Phys. Chem. Soc. St. Petersburg. 1891;23(488-517):601-11. [Link]
4. Zincke TH. Ueber die Einwirkung von Brom und von Chlor auf Phenole: Substitutionsproducte, Pseudobromide und Pseudochloride. Justus Liebigs Annalen der Chemie. 1905;343(1):75-99. [Link]
5. Allard P, Colaiacovo P. Bisphenol A. In Gupta, R. Reproductive and developmental toxicology. USA: Esliver. 2011: 673-83. [Link]
6. Shelby MD. NTP-CERHR - Monograph on the Potential Human Reproductive and Developmental Effects of Bisphenol A. Ntp cerhr mon. 2008; 22:v-ii. [pmid: 19407859]
7. Allard P, Colaiácovo MP. Bisphenol A impairs the double-strand break repair machinery in the germline and causes chromosome abnormalities. Proceedings of the National Academy of Sciences. 2010;107(47):20405-10. [doi:10.1073/pnas.1010386107]
8. Rocheste R. Bisphenol A and human health: A review of the literature. Reprod Toxicol. 2013; 42:132-155. [Link] [Link]
9. Siracusa S, Yin L, Measel E, Liang S, Yu X. Effects of bisphenol A and its analogs on reproductive health: A mini review. Reprod Toxicol. 2018; 79:96-123. [doi: 10.1016/j.reprotox.2018.06.005] [pmid: 29925041]
10. Melzer D, Rice N, Lewis C, Henley W, Galloway T. Associacion of urinary bisphenol A concentration with heart disease: evidence from NHANES 2003/06. PLoS ONE. 2010; 5: 8673. [doi:10.1371/journal.pone.0008673]
11. Hassan ZK, Elobeid MA, Virk P, Omer SA, ElAmin M, Daghestani MH, AlOlayan EM. Bisphenol A induces hepatotoxicity through oxidative stress in rat model. Oxidative medicine and cellular longevity. 2012;2012(1):194829. [doi:10.1155/2012/194829]
12. Kuiper GG, Carlsson B, Grandien K, et al. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology.1997;138:863-70. [doi:10.1210/endo.138.3.4979]
13. Doerge R, Fisher W. Background Paper on Metabolism and Toxicokinetics of Bisphenol A. In FOOD, WHO/HSE/FOS/11.1 Expert Meeting on Bisphenol A (BPA), Ottawa, Canada. 2010: 1-20. [Link]
14. Stowell L, Barvian K, Young C. et al. A role for sulfation-desulfation in the uptake of bisphenol a into breast tumor cells. Chem Biol. 2006;13:891-97. [doi:10.1016/j.chembiol.2006.06.016]
15. Zalko D, Soto M, Dolo L, Dorio C, Rathahao E, Debrauwer L, et al. Biotransformations of bisphenol A in a mammalian model: Answers and new questions raised by low-dose metabolic fate studies in pregnant CD1 mice. Environmental Health Perspectives. 2003; 111:309-19. [doi: 10.1289/ehp.5603] [DOI:10.1289/ehp.5603] [PMID] []
16. Zalko D, Jacques C, Duplan H, Bruel S, Perdu E. Viable skin efficiently absorbs and metabolizes bisphenol A. Chemosphere. 2011;82(3):424-30. [doi:10.1016/j.chemosphere.2010.09.058]
17. Wilson K, Chuang C, Lyu C, Menton R, Morgan K. Aggregate exposures of nine preschool children to persistent organic pollutants at day care and at home. J Expo Anal Environ Epidemiol.2003;13:187-202. [doi:10.1038/sj.jea.7500270]
18. Wolff S, Teitelbaum L, Windham G. et al. Pilot study of urinary biomarkers of phytoestrogens, phthalates, and phenols in girls. Environ Health Perspect. 2007; 115(1):116-21. [doi:10.1289/ehp.9488]
19. Volkel W, Colnot T, Csanady A, Filser G, Dekant W. Metabolism and kinetics of bisphenol A in humans at low doses following oral administration. Chem Res Toxicol. 2002;15:1281-7. [Link]
20. Battal D, Cok I, Unlusayin I, Tunctan B. Development and validation of an LC-MS/MS method for simultaneous quantitative analysis of free and conjugated bisphenol A in human urine. Biomed Chromatogr. 2014; 28:686-93. [doi:10.1002/bmc.3090]
21. Park K, Watanabe T, Gee J, Schenker B, Hammock D. Creatinine measurements in 24 h urine by liquid chromatography - tandem Mass Spectrometry. J Agric Food Chem.2008; 56(2):333-36. [Link]
22. Perucca J, Bouby N, Valeix P, Bankir L. Sex difference in urine concentration across differing ages, sodium intake, and level of kidney disease. Am J Physiol Reg Integr Comp Physiol. 2007;292(2): R700-5. [doi:10.1152/ajpregu.00500.2006]
23. Dekant W, Volkel W. Human exposure to bisphenol A by biomonitoring: methods, results and assessment of environmental exposures. Toxicol Appl Pharmacol. 2008; 228(1):114-34. [doi:10.1016/j.taap.2007.12.008]
24. UNEP/WHO. State of the science of endocrine disrupting chemicals. 2012. [Link]
25. Geens T, Roosens L, Neels H, Covaci A. Assessment of human exposure to bisphenol-A, triclosan and tetrabromobisphenol-A through indoor dust intake in Belgium. Chemosphere. 2009;76:755-60. [doi:10.1016/j.chemosphere.2009.05.024]
26. He Y, Miao M, Herrinton LJ, Wu C, Yuan W, Zhou Z, et al. Bisphenol A levels in blood and urine in a Chinese population and the personal factors affecting the levels. Environmental Research.2009; 109:629-33. [doi:10.1016/j.envres.2009.04.003]
27. Ye X, Bishop AM, Needham LL, Calafat AM. Automated on-line column-switching HPLC-MS/MS method with peak focusing for measuring parabens, triclosan, and other environmental phenols in human milk. Analytica Chimica Acta, 2008; 622(1-2):150-56. [doi:10.1016/j.aca.2008.05.068]
28. Health Canada - Report on Human Biomonitoring of Environmental Chemicals in Canada. Results of Canadian Health Measures Survey Cycle 1 (2007-2009). 2021. [Link]
29. Ikezuki Y, Tsutsumi O, Takai Y, Kamei Y, TaketaniY. Determination of bisphenol-A concentrations in human biological fluids reveals significant earlyprenatal exposure. Human Reproduction (Oxford, UK). 2002;17:2839-41. [doi:10.1093/humrep/17.11.2839]
30. Shiue I. Urine phthalates concentrations are higher in people with stroke: United States National Health and Nutrition Examination Surveys (NHANES), 2001-2004. European Journal of Neurology. 2013; 20(4): 728-31. [doi:10.1111/j.1468-1331.2012.03862.x]
31. Calafat M, Ye Y, Wong Y, Reidy A, Needham L. Exposure of the US population to bisphenol A and 4-tertiary-octylphenol: 2003-2004. Environ Health Perspect. 2008;116(1):39-44. [doi: 10.1289/ehp.10753] [pmid: 18197297] [doi:10.1289/ehp.10753] [pmid: 18197297]
32. Völkel W, Kiranoglu M, and Fromme H. Determination of free and total bisphenol A in urine of infants. Environmental Research. 2011; 111:143-8. [DOI:10.1016/j.envres.2010.10.001]
33. Casas L, Fernandez MF, Llop S, Guxens M, Ballester F, Olea N. et al. Urinary concentrations of phthalates and phenols in a population of Spanish pregnant women and children. Environmental International. 2011; 37:858-6. [doi:10.1016/j.envint.2011.02.012]
34. Bushnik T, Haines D, Levallois P, Levesque J. Lead and bisphenol A concentrations in the Canadian populations. Statistics of Canada Health Reproduction. 2010; 21(3):7-18. [Link]
35. Sabanayagam C, Teppala S, Shankar A. Relationship between urinary bisphenol A levels and prediabetes among subjects free of diabetes. Acta Diabetol . 2013; 50:625-31. [doi:10.1007/s00592-013-0472-z]
36. Trasande L, Attina M, and Blustein J. Association between urinary bisphenol A concentration and obesity prevalence in children and adolescents. JAMA. 2012; 308:1113-21. [Link]
37. Alshana U, Lubbad I, Goger NG, Cok I, Tamer U, Ertas N. Dispersive liquid-liquid microextraction based on solidification of floating organic drop combined with counter-electroosmotic flow normal stacking mode in capillary electrophoresis for the determination of bisphenol A in water and urine samples. Journal of Liquid Chromatography and Related Technologies. 2013; 36: 2855-70. [doi:10.1080/10826076.2012.725700]
38. Calafat M, Needham L. Factors affecting the evaluation of biomonitoring data for human exposure assessment. International Journal of Andrology. 2008 ;31(2):139-43. [doi:10.1111/j.1365-2605.2007.00826.x]
39. Brock JW, Yoshimura Y, Barr JR, Maggio VL, Graiser SR, Nakazawa H, et al. Measurement of bisphenol A levels in human urine. J Expo Sci Environ Epidemiol . 2001; 11(4):323-8. [doi:10.1038/sj.jea.7500174]
40. Wolff MS, Engel SM, Berkowitz GS, Ye X, Silva MJ, Zhu C, et al. Prenatal phenol and phthalate exposures and birth outcomes. Environ Health Perspect. 2008; 116:1092-97. [doi:10.1289/ehp.11007]
41. Zhang Z, Alomirah H, Cho HS, Li YF, Liao C, Minh TB, et al. Urinary Bisphenol A Concentrations and Their Implications for Human Exposure in Several Asian Countries. Environ Sci Technol. 2011 ;45(16):7044-50. [doi:10.1021/es200976k]
42. Becker K, Göen T, Seiwert M, Conrad A, Pick-Fub H, Müller J, et al. Phthalate metabolites and bisphenol A in urine of German children. International Journal of Hygiene and Environmental Health. 2009; 212:685-92. [doi:10.1016/j.ijheh.2009.08.002]
43. Liao C, Kannan K. Determination of free and conjugated forms of bisphenol A in human urine and serum by liquid chromatography & tandem mass spectrometry. Environ Sci Technol. 2012; 46(9): 5003-9. [doi:10.1021/es300115a]
44. Takeuchi T, Tsutsumi O. Serum bisphenol-A concentrations showed gender differences, possibly linked to androgen levels. Biochemical and Biophysical Research Communications. 2002; 291(1):76-8. [doi:10.1006/bbrc.2002.6407]
45. Huang P, Liu ZH, Yuan SF, Yin H, Dang Z, Wu PX. Worldwide human daily intakes of bisphenol A (BPA) estimated from global urinary concentration data (2000-2016) and its risk analysis. Environ Pollut. 2017; 230:143-52. [doi:10.1016/j.envpol.2017.06.026]
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
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