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Volume 18, Issue 2 (May 2024)
IJT 2024, 18(2): 78-83 |
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Ethics code: IR.QUMS.REC.1399.546
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Asoudeh-Fard A, Najafipour R, Hosseini A H, Razi F, Salahshourifar I, Zareian Jahromi M, et al . Novel Effects of Phycocyanin Extract on the Regulation of Apoptosis Genes in Human Colorectal Cancer Cells. IJT 2024; 18 (2) :78-83
URL: http://ijt.arakmu.ac.ir/article-1-1294-en.html
URL: http://ijt.arakmu.ac.ir/article-1-1294-en.html
Abbas Asoudeh-Fard1 
, Reza Najafipour2 , Amir Hadi Hosseini3 , Faezeh Razi4 , Iman Salahshourifar5 , Mohammad Zareian Jahromi6 , Asghar Parsaei7 , Hossein Piri *8
, Reza Najafipour2 , Amir Hadi Hosseini3 , Faezeh Razi4 , Iman Salahshourifar5 , Mohammad Zareian Jahromi6 , Asghar Parsaei7 , Hossein Piri *8
1- Laboratory for Vascular Translation Science, Cardiovascular Bioengineering, University Sorbonne, Paris North, Paris, France, and the Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
2- Genetics Research Center, University of Social Welfare and Rehabilitation Science, Tehran, Iran
3- Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
4- Faculty of Convergent Sciences and Technologies, Islamic Azad University of Science and Research, Tehran, Iran
5- Assistant Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
6- Assistant Professor, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
7- Rayan Novin Pajoohan Pars, Biotechnology Company Rep. and Shiraz University of Medical Sciences, Shiraz, Iran
8- Associate Professor, Department of Biochemistry and Genetics, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran ,hosseinpiry@gmail.com
2- Genetics Research Center, University of Social Welfare and Rehabilitation Science, Tehran, Iran
3- Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
4- Faculty of Convergent Sciences and Technologies, Islamic Azad University of Science and Research, Tehran, Iran
5- Assistant Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
6- Assistant Professor, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
7- Rayan Novin Pajoohan Pars, Biotechnology Company Rep. and Shiraz University of Medical Sciences, Shiraz, Iran
8- Associate Professor, Department of Biochemistry and Genetics, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran ,
Abstract: (891 Views)
Background: Cancer is a major cause of death worldwide, second only to cardiovascular disease. Many factors influence the development of cancer, including environmental, genetic, and lifestyle factors. Although the prognosis of colon cancer treatment has improved significantly in recent years, there is still much clinical need to explore new treatment strategies with anticancer and immune-enhancing effects. Phycocyanin (PC) is an important compound isolated from blue-green algae and a well-known antioxidant that suppresses oxidative reactions in cells. It also has other useful functions, such as high efficiency, low toxicity, and antitumor properties. We investigated the potential antiapoptotic effect of PC in vitro on human normal and cancer cell lines.
Methods: Phycocyanin extracted from Streptomyces platensis algae has the potential to become a turning point in pharmaceutical research. In this study, PC was used as a natural anticancer agent and apoptosis activator to increase the expression of the PTEN and Bax genes in HT-29 cancer and normal cells (HUVEC). The effect of treatment with PC was evaluated using an MTT assay and flow cytometry.
Results: The results showed that PC can have apoptotic effects on the human colon cancer cell line (HT-29) by up-regulating PTEN and Bax genes and down-regulating AKT and BCL-2 genes.
Conclusion: Based on the findings, PC can be considered a herbal treatment and a useful supplement in the management of human colorectal cancer.
Methods: Phycocyanin extracted from Streptomyces platensis algae has the potential to become a turning point in pharmaceutical research. In this study, PC was used as a natural anticancer agent and apoptosis activator to increase the expression of the PTEN and Bax genes in HT-29 cancer and normal cells (HUVEC). The effect of treatment with PC was evaluated using an MTT assay and flow cytometry.
Results: The results showed that PC can have apoptotic effects on the human colon cancer cell line (HT-29) by up-regulating PTEN and Bax genes and down-regulating AKT and BCL-2 genes.
Conclusion: Based on the findings, PC can be considered a herbal treatment and a useful supplement in the management of human colorectal cancer.
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Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
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