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Volume 18, Issue 3 (July 2024)
IJT 2024, 18(3): 130-137 |
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Ethics code: Medical/Health Research Bioethics Commission, Faculty of Medicine, Universitas Islam Sultan Agung, N
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Marianti A, Amalina N D, Chairani Dimarti S, Anindita R, Dyarizky Ramadhan M D, Bintang Ramadani M. Protective Effects of Chitosan Nanoparticles against Hepatic and Renal Damage in Rats with Chronic Lead Poisoning. IJT 2024; 18 (3) :130-137
URL: http://ijt.arakmu.ac.ir/article-1-1316-en.html
URL: http://ijt.arakmu.ac.ir/article-1-1316-en.html
Aditya Marianti *1 
, Nur Dina Amalina2 , Safira Chairani Dimarti3 , Rahma Anindita4 , M Daffa Dyarizky Ramadhan4 , Mutiara Bintang Ramadani4
, Nur Dina Amalina2 , Safira Chairani Dimarti3 , Rahma Anindita4 , M Daffa Dyarizky Ramadhan4 , Mutiara Bintang Ramadani4
1- Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Semarang, Semarang, Indonesia , aditya.marianti.am@mail.unnes.ac.id
2- Department of Pharmacy, Faculty of Medicine, Universitas Negeri Semarang, Semarang, Indonesia
3- Department of Nutrition, Faculty of Medicine, Universitas Negeri Semarang, Semarang, Indonesia
4- Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Semarang, Semarang, Indonesia
2- Department of Pharmacy, Faculty of Medicine, Universitas Negeri Semarang, Semarang, Indonesia
3- Department of Nutrition, Faculty of Medicine, Universitas Negeri Semarang, Semarang, Indonesia
4- Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Semarang, Semarang, Indonesia
Abstract: (780 Views)
Background: Lead exposure inflicts severe damage to various body organs. The present study sought to assess the effect of chitosan nanoparticles (NPs) on the prevention of hepatic and renal damage in rats with chronic lead poisoning. The indicators were the levels of oxidative stress and proinflammatory cytokines expression.
Methods: We used Rattus norvegicus as the animal model. They were assigned to three groups: negative control, positive control, and treatment (n=8 each). The negative control and treatment groups were the models for chronic lead poisoning, and the serum lead levels were determined by atomic absorption spectrometry. The treatment group was orally administered chitosan NPs at 64 mg/kg for 30 days. The reactive oxygen species (ROS) were measured using 2',7’-dichlorodihydrofluorescein diacetate flow cytometry. The hepatic and renal TNF-α and IL-6 gene expressions were also analyzed.
Results: The mean serum lead level was 0.52 mg/L, validating the rats as the lead poisoning model. The results indicated that the liver and kidneys in the treatment group had the lowest ROS and TNF-α levels compared to those in other groups. The treatment rats had a lower hepatic IL-6 level compared to those in positive controls, although this was higher than that in the negative controls. The renal IL-6 level in the treatment group was the highest among all groups.
Conclusion: As evidenced by the results of this study, chitosan NPs had a protective effect on the liver and kidneys of rats during chronic lead poisoning by reducing the ROS and TNF-α levels; nonetheless, it did not suppress the renal IL-6 expression.
Methods: We used Rattus norvegicus as the animal model. They were assigned to three groups: negative control, positive control, and treatment (n=8 each). The negative control and treatment groups were the models for chronic lead poisoning, and the serum lead levels were determined by atomic absorption spectrometry. The treatment group was orally administered chitosan NPs at 64 mg/kg for 30 days. The reactive oxygen species (ROS) were measured using 2',7’-dichlorodihydrofluorescein diacetate flow cytometry. The hepatic and renal TNF-α and IL-6 gene expressions were also analyzed.
Results: The mean serum lead level was 0.52 mg/L, validating the rats as the lead poisoning model. The results indicated that the liver and kidneys in the treatment group had the lowest ROS and TNF-α levels compared to those in other groups. The treatment rats had a lower hepatic IL-6 level compared to those in positive controls, although this was higher than that in the negative controls. The renal IL-6 level in the treatment group was the highest among all groups.
Conclusion: As evidenced by the results of this study, chitosan NPs had a protective effect on the liver and kidneys of rats during chronic lead poisoning by reducing the ROS and TNF-α levels; nonetheless, it did not suppress the renal IL-6 expression.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
