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Volume 19, Issue 4 (October 2025)
IJT 2025, 19(4): 0-0 |
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Tewari I, Ram V, Singh S, F Singh M. Toxicity and fertility studies of Cornus capitata Well. fruit extract in Zebrafish Model. IJT 2025; 19 (4)
URL: http://ijt.arakmu.ac.ir/article-1-1482-en.html
URL: http://ijt.arakmu.ac.ir/article-1-1482-en.html
1- Pharmacology, School of Pharmaceutical Sciences, Sardar Bhagwan Singh University, Dehradun, Uttarakhand, India
2- SBS UNIVERSITY, DEHRADUN
3- Siddhartha Institute of Pharmacy, Dehradun
4- College of Pharmacy, COER University, Roorkee, 247667, Uttarakhand ,mamta_fr2002@yahoo.co.in
2- SBS UNIVERSITY, DEHRADUN
3- Siddhartha Institute of Pharmacy, Dehradun
4- College of Pharmacy, COER University, Roorkee, 247667, Uttarakhand ,
Abstract: (15 Views)
Background: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder, demanding the development of safe and effective treatment. Fruits of Cornus capitata Wall. are particularly known for their anticancer, antidiabetic, and antioxidant effects.
Objective: This study aimed to evaluate the toxicity and therapeutic potential of the methanolic extract of Cornus capitata Well. fruits (MECCF) using fish embryo toxicity assay and testosterone-induced PCOS in zebrafish model.
Methods: Phytochemical screening and antioxidant activity (DPPH and Ferric reductase assay) of MECCF were evaluated. Embryo toxicity was evaluated in zebrafish embryos, on the basis of survival and morphological analyses using different concentrations of MECCF (5, 2.5, 1.25, 0.625, 0.3125 & 0.156 g/L). Adult zebrafish (3-8 months) were given testosterone (100ng) dissolved in distilled water containing 0.1% dimethyl sulfoxide followed by MECCF treatment (31.25 mg/L, 62.25 mg/L).
Results: In vitro antioxidant studies revealed a significant free radical scavenging property of MECCF. Embryo toxicity assay revealed that MECCF concentrations of 5, 2.5, 1.25, 0.625 g/L showed the highest rates of lethality. 0.3125, and 0.156 g/L were found to have no lethal effects even after 9 hours post-fertilization. Exposure to 62.25 mg/L MECCF in adult zebrafish showed significant changes in testosterone levels, gonado somatic index and ovarian health.
Conclusion: MECCF exhibits potent antioxidant activity and improves testosterone-induced PCOS symptoms in adult zebrafish, with low toxicity at appropriate concentrations in zebrafish embryos. Observed therapeutic effects may be attributed presence of triterpenoids and flavonoids, supporting its potential as a natural candidate for PCOS symptoms management.
Objective: This study aimed to evaluate the toxicity and therapeutic potential of the methanolic extract of Cornus capitata Well. fruits (MECCF) using fish embryo toxicity assay and testosterone-induced PCOS in zebrafish model.
Methods: Phytochemical screening and antioxidant activity (DPPH and Ferric reductase assay) of MECCF were evaluated. Embryo toxicity was evaluated in zebrafish embryos, on the basis of survival and morphological analyses using different concentrations of MECCF (5, 2.5, 1.25, 0.625, 0.3125 & 0.156 g/L). Adult zebrafish (3-8 months) were given testosterone (100ng) dissolved in distilled water containing 0.1% dimethyl sulfoxide followed by MECCF treatment (31.25 mg/L, 62.25 mg/L).
Results: In vitro antioxidant studies revealed a significant free radical scavenging property of MECCF. Embryo toxicity assay revealed that MECCF concentrations of 5, 2.5, 1.25, 0.625 g/L showed the highest rates of lethality. 0.3125, and 0.156 g/L were found to have no lethal effects even after 9 hours post-fertilization. Exposure to 62.25 mg/L MECCF in adult zebrafish showed significant changes in testosterone levels, gonado somatic index and ovarian health.
Conclusion: MECCF exhibits potent antioxidant activity and improves testosterone-induced PCOS symptoms in adult zebrafish, with low toxicity at appropriate concentrations in zebrafish embryos. Observed therapeutic effects may be attributed presence of triterpenoids and flavonoids, supporting its potential as a natural candidate for PCOS symptoms management.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
