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Volume 6, Issue 18 (Autumn 2012)                   IJT 2012, 6(18): 699-703 | Back to browse issues page

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Sattar S, Asghari G, Ehsanpour A A. Biotechnological Reduction of Tobacco (Nicotiana Tabacum L.) Toxicity. IJT. 2012; 6 (18) :699-703
URL: http://ijt.arakmu.ac.ir/article-1-149-en.html
1- Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
2- Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. , asghari@pharm.mui.ac.ir
3- Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran.
Abstract:   (7583 Views)
BACKGROUND: Nicotiana tobacco contains large amounts of alkaloid nicotine. Tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. Reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. Utilizing the new methods of genetic engineering can modify nicotine levels in the plant. In this study, through transferring the blocking gene, the pathway of nicotine biosynthesis was blocked to produce transgenic tobacco with low levels of nicotine.
Methods: Transgenic plants carrying T DNA, and non-transgenic plants were grown on MS medium. Then their leaves were dried and powdered. The plants were extracted with alkali solution. Eventually, the nicotine content of the extract were analyzed using GC.
Results: The analysis of extracts showed a reduction in the nicotine content of the transgenic plant (contain T-DNA) in comparison with non-transgenic plants.
Conclusion: Tobacco with lower nicotine reduction can reduce the toxic effects of smoking on smokers and can facilitate withdrawal from it.
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Type of Study: Research | Subject: Special

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