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1- Toxicology, Department of Marine Biology, Faculty of Marine Science and oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
2- Department of Marine Biology, Faculty of Marine Science and oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. ,salamatnegin@yahoo.com
3- Department of Basic Sciences, Abadan University of Medical Sciences, Abadan, Iran.
2- Department of Marine Biology, Faculty of Marine Science and oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. ,
3- Department of Basic Sciences, Abadan University of Medical Sciences, Abadan, Iran.
Abstract: (12 Views)
The present study was carried out to identify potential effects of dexamethasone (DEX) on antioxidant parameters of Acanthopagrus arabicus, in vitro. Cultured hepatocytes from A. arabicus were exposed to 3, 3×10, 3×102 and 3×103µg/ ml of DEX for 48 h. Then, the toxic effects of DEX on the total antioxidant capacity (TAC), glutathione enzyme activity (GSH), lipid peroxidation (LPO), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured at 12, 24 and 48h of the experiment. The results indicated that the sensitivity of cultivated liver cells to DEX increased in a dose-dependent manner. The levels of TAC and GSH activity increased significantly in cells exposed to 3 and 3×10 µg/ml of DEX after 48 h of experiment. In contrast, higher concentrations of DEX (3×102 and 3×103 µg/ml) induced a significant decrease in TAC value and GSH activity in treated hepatocytes throughout the experiment. The LPO, AST, ALT, and ALP activities increased dose and time-dependently in cultivated hepatocytes exposed to 3×102 and 3×103 µg/ml and reached their peak following 48 h of exposure. In conclusion, DEX can suppress the antioxidant defense system, as evidenced by the significant increase in lipid peroxides and decrease in antioxidant factors in the liver.
Keywords: Acanthopagrus arabicus, Dexamethasone, Glutathione, Liver enzymes, Total antioxidant capacity
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
