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Volume 7, Issue 23 (Winter 2014)
IJT 2014, 7(23): 944-951 |
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Hashemi S A, Allameh A , Daraei B, Moradi Peynevandi K, Pashazadeh R. The Effect of Rebadioside A on Attenuation of Oxidative Stress in Kidney of Mice under Acetaminophen Toxicity. IJT 2014; 7 (23) :944-951
URL: http://ijt.arakmu.ac.ir/article-1-270-en.html
URL: http://ijt.arakmu.ac.ir/article-1-270-en.html
Seyed Ali Hashemi1 
, Abdolamir Allameh * 2, Bahram Daraei3 , Kamran Moradi Peynevandi4 , Ramin Pashazadeh5
, Abdolamir Allameh * 2, Bahram Daraei3 , Kamran Moradi Peynevandi4 , Ramin Pashazadeh5
1- Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran.
2- Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran. ,allameha@modares.ac.ir
3- Department of Toxicology, Tarbiat Modares University, Tehran, Iran.
4- Department of Plant Physiology, Tarbiat Modares University, Tehran, Iran.
5- Department of Chemistry, Tarbiat Modares University, Tehran, Iran.
2- Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran. ,
3- Department of Toxicology, Tarbiat Modares University, Tehran, Iran.
4- Department of Plant Physiology, Tarbiat Modares University, Tehran, Iran.
5- Department of Chemistry, Tarbiat Modares University, Tehran, Iran.
Abstract: (7255 Views)
Background: Acetaminophen (APAP) overdose causes renal and hepatic injury. It is also believed that oxidative stress has a pivotal role in APAP-induced renal injury. Therefore, protective effects of different antioxidants have been examined in APAP-induced renal and hepatic toxicity models. Stevia rebadiana is a plant with a high degree of natural antioxidant activity in its leaf extract. The aim of this study was to investigate the possible protective effects of rebadioside A one of the main components of stevia extract, on APAP-induced oxidative stress in kidney of mice.
Methods: Oxidative stress was induced in kidney of BALB/c mice by the intraperitoneal (i.p.) administration of a single dose of 300 mg/kg APAP. Some of these mice also received rebadioside A (700 mg/kg) (i.p.) 30 minutes after APAP injection. Two and six hours after APAP injection, all mice were sacrificed and malondialdehyde (MDA), glutathione (GSH), free APAP, and glutathione conjugated of APAP (APAP-GSH) were determined in the kidney tissue.
Results: GSH depletion and MDA levels significantly (P<0.05) increased in mice treated with either APAP or APAP plus Rebadioside A, respectively in 2 and 6 hours intervals after APAP administration. Significantly (P<0.05) higher levels of free APAP and APAP-GSH levels detected in kidney of mice administrated with APAP plus rebadioside A compared to APAP treated ones.
Conclusion: Rebadioside A may be a potential compound in alleviation of APAP-induced oxidative stress in kidney of mice after APAP overdoses.
Methods: Oxidative stress was induced in kidney of BALB/c mice by the intraperitoneal (i.p.) administration of a single dose of 300 mg/kg APAP. Some of these mice also received rebadioside A (700 mg/kg) (i.p.) 30 minutes after APAP injection. Two and six hours after APAP injection, all mice were sacrificed and malondialdehyde (MDA), glutathione (GSH), free APAP, and glutathione conjugated of APAP (APAP-GSH) were determined in the kidney tissue.
Results: GSH depletion and MDA levels significantly (P<0.05) increased in mice treated with either APAP or APAP plus Rebadioside A, respectively in 2 and 6 hours intervals after APAP administration. Significantly (P<0.05) higher levels of free APAP and APAP-GSH levels detected in kidney of mice administrated with APAP plus rebadioside A compared to APAP treated ones.
Conclusion: Rebadioside A may be a potential compound in alleviation of APAP-induced oxidative stress in kidney of mice after APAP overdoses.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
