[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 1, Number 2 (Summer 2007) ::
IJT 2007, 1(2): 6-6 Back to browse issues page
Heavy Metal Induced Cell Necrosis: Involves Apoptosis Death Signals Initiated by Mitochondrial Injury
Jalal Pourahmad *, Farzaneh Jokar, Mariam Rabiei, Peter J.O Brien
Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Beheshti Medical University, Tehran, Iran. , j.pourahmadjaktaji@utoronto.ca
Abstract:   (8306 Views)
Introduction: Severe industrial diseases result from the hepatic accumulation of mercury, cadmium or chromium in humans and on the other hand cadmium and dichromate and mercuric salts may induce lung or kidney cancer. Acute or chronic CdCl2, HgCl2 or dichromate administration induces hepatic and nephrotoxicity in rodents. Oxidative stress is often cited as a possible cause of metal induced cell death but the death signaling pathways involved have not yet been well investigated.
Method and Materials: To search for death signaling mechanisms we used accelerated cytotoxicity mechanism screening techniques (ACMS) on isolated rat hepatocytes as our cellular model.
Results: Adding the CdC12, HgC12 or K2Cr2O7 to isolated hepatocytes caused a rapid increase in reactive oxygen species ("ROS") formation and a decline in mitochondrial membrane potential. Then lipid per-oxidation and cell-lysis ensued. Cytotoxicity was prevented by "ROS" scavengers and various inhibitors of the mitochondrial permeability transition (MPT) e.g. cyclosporin A, carnitine or trifluoperazine. Antioxidants prevented hepatocyte lysis induced by CdC12 , K2Cr207 but not HgC12.
Conclusion: Hepatocyte lysis was also prevented by various apoptosis inhibitors e.g. cycloheximide, dactinomycin and a tetrapeptide caspase 3 inhibitor which suggested that metal induced hepatocyte lysis involves apoptotic death signals initiated by MPT and "ROS".
Keywords: Chromium, Cadmium, Mercury, MPT, Lipid per oxidation, ROS, Apoptosis, Necrosis .
Full-Text [PDF 9 kb]   (1301 Downloads)    
Type of Study: Research | Subject: General
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pourahmad J, Jokar F, Rabiei M, Brien P J. Heavy Metal Induced Cell Necrosis: Involves Apoptosis Death Signals Initiated by Mitochondrial Injury. IJT. 2007; 1 (2) :6-6
URL: http://ijt.arakmu.ac.ir/article-1-31-en.html
Volume 1, Number 2 (Summer 2007) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
Persian site map - English site map - Created in 0.047 seconds with 792 queries by yektaweb 3471