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Volume 9, Issue 30 (Autumn 2015)
IJT 2015, 9(30): 1402-1406 |
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Yousofvand N, Fahim M. Effects of Chronic Exposure to Sodium Arsenate on Kidney of Rats. IJT 2015; 9 (30) :1402-1406
URL: http://ijt.arakmu.ac.ir/article-1-458-en.html
URL: http://ijt.arakmu.ac.ir/article-1-458-en.html
1- Department of Biology, University of Razi, Kermanshah, Iran. , yousofvand@razi.ac.ir
2- Department of Physiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
2- Department of Physiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
Abstract: (4648 Views)
Background: In the present study, histopathological effects of chronic exposure to sodium arsenate in drinkable water were studied on a quantity of organs of rat.
Methods: Rats were divided into two groups, group I served as control group, were main-tained on deionized drinkable water for 2 months, and group II the study group were given 60 g/ml of sodium arsenate in deionized drinkable water for 2 months. Blood and urine samples from two groups of animals were collected under anesthesia and the animals were sacrificed under deep anesthesia (a-chloralose, 100 mg/kg, I.P). Their kidney, liver, aorta, and heart were dissected out and cleaned of surrounding connective tissue. The organs were kept in formaldehyde (10%) for histopathologic examination. Serum and urine samples from two groups were collected and analyzed for arsenic level. Total quantity of arsenic in serum and urine of animal was measured through graphic furnace atomic absorption spectrometry (GF-AAS).
Results: Examination with light microscopy did not show any visible structural changes in the aorta, myocardium, and liver of chronic arsenic treated animals.However, a significant effect was observed in the kidneys of chronic arsenic treated rats showing distinct changes in proxi-mal tubular cells. There was high concentration of arsenic in serum and urine of arsenic ex-posed animals (group II) significantly (P<0.001).
Conclusion: Swollen tubular cells in histopathologic study of kidney may suggest toxic effects of arsenic in the body.
Methods: Rats were divided into two groups, group I served as control group, were main-tained on deionized drinkable water for 2 months, and group II the study group were given 60 g/ml of sodium arsenate in deionized drinkable water for 2 months. Blood and urine samples from two groups of animals were collected under anesthesia and the animals were sacrificed under deep anesthesia (a-chloralose, 100 mg/kg, I.P). Their kidney, liver, aorta, and heart were dissected out and cleaned of surrounding connective tissue. The organs were kept in formaldehyde (10%) for histopathologic examination. Serum and urine samples from two groups were collected and analyzed for arsenic level. Total quantity of arsenic in serum and urine of animal was measured through graphic furnace atomic absorption spectrometry (GF-AAS).
Results: Examination with light microscopy did not show any visible structural changes in the aorta, myocardium, and liver of chronic arsenic treated animals.However, a significant effect was observed in the kidneys of chronic arsenic treated rats showing distinct changes in proxi-mal tubular cells. There was high concentration of arsenic in serum and urine of arsenic ex-posed animals (group II) significantly (P<0.001).
Conclusion: Swollen tubular cells in histopathologic study of kidney may suggest toxic effects of arsenic in the body.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
