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Volume 10, Issue 4 (July-August 2016)                   IJT 2016, 10(4): 13-20 | Back to browse issues page

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Hassanshahian M. Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils. IJT. 2016; 10 (4) :13-20
URL: http://ijt.arakmu.ac.ir/article-1-487-en.html
Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran. , mshahi@uk.ac.ir
Abstract:   (3596 Views)

Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran.

Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on amplifying of 16s rDNA.

Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environment. Three diazinon-degrading bacteria strains belong to: Pseudomonas putida strain D3, P. fluorescens strain D1 and Achromobacter piechaudii strain D8. The best degrading strain (D1), up to 100 ppm, illustrated a good growth, whereas more than this concentration, the growth was reduced. The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.

Conclusion: The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.

Using these strains and other biological reclamation methods we can eliminate bio-environmental problems.

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Type of Study: Research | Subject: Special

1. Drufovka K, Danevcic T, Trebs P, Stopar D. Microorganisms trigger chemical degradation of diazinon. Inter Biodeterior Biodegrad 2008; 62: 293–6. [DOI:10.1016/j.ibiod.2008.02.003]
2. Singh BK, Walker A. Microbial degradation of organophosphorus compounds. FEMS Microbiol Rev 2006; 30: 428–71. [DOI:10.1111/j.1574-6976.2006.00018.x]
3. Sanchez ME, Estrada IB, Martinez O, Martin-Villacorta J, Aller A, Moran A. Influence of the application of sewage sludge on the degradation of pesticides in the soil. Chemosphere 2004; 57: 673–9. [DOI:10.1016/j.chemosphere.2004.07.023]
4. Yasouri FN. Plasmid mediated degradation of diazinon by three bacterial strains Pseudomonas sp., Flavobacterium sp. and Agrobacterium sp. Asian J Chem 2006; 18: 2437–44.
5. Deshpande N. Biodegradation of Dimethoate-a carbamate group of organophosphorus insecticides. Lett Appl Microbiol 2002; 33: 275-9. [DOI:10.1046/j.1472-765X.2001.00995.x]
6. Kanekar P, Bhadbhade B. Biodegreadtion of Organophosphorus Pseticides. Proc Indian natn Sci Acad 2004; 70: 57-70.
7. Cycoń M, Wójcik M, Piotrowska Z. Biodegradation of the organophosphorus insecticide diazinon by Serratia sp. and Pseudomonas sp. and their use in bioremediation of contaminated soil. Chemosphere 2009; 76: 494-501. [DOI:10.1016/j.chemosphere.2009.03.023]
8. Holt SG, Kriey NR, Sneath PHA, Staley JT, Williams ST. Bergy,s manual of determinative for bacteriology 1998; Williams and Wilkins, New York.
9. Winnepenninckx B, Backeljau T, Dewachter R. Extraction of high molecular weight DNA from molluscs. Trends Genet 1993; 9: 407-11. [DOI:10.1016/0168-9525(93)90102-N]
10. Maidak BL, Olsen GJ, Larsen N. The RDP Ribosomal Database Project. Nucleic Acids Res 1997; 25: 109-1 11.
11. Troussellier M, Got P, Mboup M, Corbin D, Giuliano L, Cappello S, Bouvy M. Daily bacterio plankton in a sub-Saharan estuary (Senegal River, West Africa): a mesocosm study. Aquat Microb Ecol 2005: 40: 13-24. [DOI:10.3354/ame040013]
12. Yakimov MM, Cappello S, Crisafi E, Tursi A. Phylogenetic survey of metabolically active microbial communities associated with the deep-sea coral Lophelia pertusa from the Apulian Plateau, Central Mediterran Sea. Deep Sea Res part I (2006); 53: 62-75. [DOI:10.1016/j.dsr.2005.07.005]
13. Abo A, Aly E. Biodegradation of Diazinon by Serratia marcescens DI101 & its use in bioremediation of contaminated Environment. J Microbial Biotecnol 2011; 21: 71-80. [DOI:10.4014/jmb.1007.07024]
14. Rosenberg A, Alexander M. Microbial cleavage of various Organophosphorus insecticides. Apple Environ Microbiol 1979; 37: 886-91.
15. Beynon K, Hutson D, Wright A. The metabolism & degradation of Vinyl phosphate insecticides. Res Rev 1973; 47: 55-142. [DOI:10.1007/978-1-4615-8488-9_2]
16. Bayat Z, Hassanshahian M, Askeri Hesni M. Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf. Mar Pollut Bull 2015; 101 (1): 85–91. [DOI:10.1016/j.marpolbul.2015.11.021]
17. Emtiazi G, Saleh T, Hassanshahian M. The effect of bacterial glutathione S-transferase on morpholine Degradation. Biotechnol J. 2009; 4: 202–5. [DOI:10.1002/biot.200800238]
18. Hassanshahian M, Zeynalipour M.S, Hosseinzadeh Musa F. Isolation and characterization of crude oil degrading bacteria from the Persian Gulf (Khorramshahr provenance). Mar Pollut Bull 2014; 82: 39–44. [DOI:10.1016/j.marpolbul.2014.03.027]
19. Hassanshahian M, Emtiazi G, Cappello S. Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. Mar Pollut Bull 2102; 64: 7–12. [DOI:10.1016/j.marpolbul.2011.11.006]
20. Hassanshahian M. Isolation and characterization of biosurfactant producing bacteria from Persian Gulf (Bushehr provenance). Mar Pollut Bull 2014; 86: 361–6. [DOI:10.1016/j.marpolbul.2014.06.043]

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