[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 10, Issue 5 (September-October 2016) ::
IJT 2016, 10(5): 17-22 Back to browse issues page
Helianthus annuus Leaf Ameliorates Postprandial Hyperglycaemia by Inhibiting Carbohydrate Hydrolyzing Enzymes Associated with Type-2 Diabetes
Oluwafemi Adeleke Ojo * , Adebola Busola Ojo , Basiru Ajiboye , Israel Olayide , Adewale Fadaka
Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Nigeria. , oluwafemiadeleke08@gmail.com
Abstract:   (939 Views)

Background: Diabetes mellitus is a chronic disease. Decreasing postprandial hyperglycemia by retarding glucose absorption through inhibiting carbohydrates digesting enzymes (α-amylase and α-glucosidase) is one of many approaches used for the management of this disease. This study was aimed at evaluating the normoglycaemic potential of Helianthus annuus leaf.

Methods: The effect of the in vitro inhibitory of different extracts (acetone, ethyl acetate and hexane) of the plant was assessed on the activities of diabetes-related enzymes (α-amylase and α-glucosidase).

Results: The hexane extract of H. annuus leaf displayed the best inhibitory activity against α-amylase and α-glucosidase as indicated by the IC50 values (3.92 ± 0.02 mg mL-1) and (3.29 ± 0.12 mg mL-1), respectively. Lineweaver-Burk plot of inhibition of α-amylase and α-glucosidase by this extract showed that it was competitive and non-competitive mode, respectively.

Conclusion: H. annuus leaf possesses hypoglycaemic potential which may be due to the inhibition of pancreatic α-amylase and intestinal α-glucosidase.

Keywords: α-Amylase, α-Glucosidase, Helianthus Annuus, Postprandial Hyperglycaemia.
Full-Text [PDF 1546 kb]   (383 Downloads)    
Type of Study: Research | Subject: General
1. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes estimates for the year 2000 and projections for 2030. Diabetes care 2004;27(5):1047-53. [DOI:10.2337/diacare.27.5.1047]
2. International Diabetes Federation (IDF). Diabetes Atlas. 5 th ed. Brussels: Belgium, 2011. P.14-5.
3. Ali MS, Jahangir M, ul Hussan SS, Choudhary MI. Inhibition of α-glucosidase by oleanolic acid and its synthetic derivatives. Phytochemistry 2002;60(3):295-9. [DOI:10.1016/S0031-9422(02)00104-8]
4. Cai H, Mcneilly AS, Luttrell LM, Martin B. Endocrine function in aging. Int J Endocrinol 2012; 2012:872478-81. [DOI:10.1155/2012/872478]
5. Duke J, Wain K. Medicinal plants of the world. Computer index with more than 85000 entries. Encyclopaedia Americana, Grolier 1981;3:25-6.
6. Ngondi JL, Fossouo Z, Djiotsa EJ, Oben J. Glycaemic variations after administration of Irvingia gabonensis seeds fractions in normoglycemic rats. Afr J Tradit Complement Altern Med 2006;3(4):94-101. [DOI:10.4314/ajtcam.v3i4.31181]
7. Saini S, Sharma S. Antidiabetic effect of Helianthus annuus L, seeds ethanolic extract in streptozotocinnicotinamide induced type 2 diabetes mellitus. Int J Pharm Pharm Sci 2013;5(2):382-7.
8. Sofowora A. Screening plants for bioactive agents. In: Medicinal plants and traditional medicinal in Africa. Nigeria: Ibadan;1993.
9. Evans W. Trease and Evans pharmacognosy. WB Saunders and Company, London. 2002.
10. Shai LJ, Masoko P, Mokgotho MP, Magano SR, Mogale A, Boaduo N, et al. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa. Afr J Bot 2010;76(3):465-70. [DOI:10.1016/j.sajb.2010.03.002]
11. Ali H, Houghton P, Soumyanath A. α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 2006;107(3):449-55. [DOI:10.1016/j.jep.2006.04.004]
12. Ademiluyi AO, Oboh G. Soybean phenolic-rich extracts inhibit key-enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin I converting enzyme) in vitro. Exp Toxicol Pathol 2013;65(3):305-9. [DOI:10.1016/j.etp.2011.09.005]
13. Choudhary MI, Adhikari A, Rasheed S, Marasini BP, Hussain N, Kaleem WA. Cyclopeptide alkaloids of Ziziphus oxyphylla Edgw as novel inhibitors of α-glucosidase enzyme and protein glycation. Phytochem Lett 2011;4(4):404-6. [DOI:10.1016/j.phytol.2011.08.006]
14. Ojo OA, Oloyede OI, Olarewaju OI, Ojo AB, Ajiboye BO, Onikanni SA. Toxicity studies of the crude aqueous leaves extracts of Ocimum gratissimum in albino rats. IOSR J Environ Sci Toxicol Food Technol 2013; 6(4) :34-9. [DOI:10.9790/2402-0643439]
15. Huang TH, Peng G, Kota BP, Li GQ, Yamahara J, Roufogalis BD, et al. Anti-diabetic action of Punica granatum flower extract: activation of PPAR-γ and identification of an active component. Toxicol Appl Pharmacol 2005;207(2):160-9. [DOI:10.1016/j.taap.2004.12.009]
16. Ajiboye BO, Ibukun EO, Edobor G, Ojo OA, Onikanni SA. Qualitative and Quantitative analysis of phytochemicals in Senecio biafrae leaf. Int J Inv Pharm Sci 2013; 1(5): 428-32.
17. Krishnaiah D, Devi T, Bono A, Sarbatly R. Studies on phytochemical constituents of six Malaysian medicinal plants. JMPR 2009;3(2):067-72.
18. Lee K-T, Jung TW, Lee H-J, Kim S-G, Shin Y-S, Whang W-K. The antidiabetic effect of ginsenoside Rb2 via activation of AMPK. Arch Pharm Res 2011;34(7):1201-8. [DOI:10.1007/s12272-011-0719-6]
19. Eu CHA, Lim WYA, Ton SH, bin Abdul Kadir K. Glycyrrhizic acid improved lipoprotein lipase expression, insulin sensitivity, serum lipid and lipid deposition in high-fat diet-induced obese rats. Lipids Health Dis 2010;9(1):1-9. [DOI:10.1186/1476-511X-9-81]
20. Kimura Y, Araki Y, Takenaka A, Igarashi K. Protective effects of dietary nasunin on paraquat-induced oxidative stress in rats. Biosci Biotechnol Biochem 1999;63(5):799-804. [DOI:10.1271/bbb.63.799]
21. Kwon YI, Apostolidis E, Shetty K. Inhibitory potential of wine and tea against α‐Amylase and α‐Glucosidase for management of hyperglycemia linked to type 2 diabetes. J Food Biochem 2008;32(1):15-31. [DOI:10.1111/j.1745-4514.2007.00165.x]
22. Kazeem MI, Ogungbe SM, Saibu GM, Aboyade OM. In vitro study on the hypoglycemic potential of Nicotiana tabacum leaf extracts. Bangladesh J Pharmacol 2014;9(2):140-5. [DOI:10.3329/bjp.v9i2.17540]
23. Mogale M, Lebelo SL, Thovhogi N, De Freitas A, Shai L. α-Amylase and α-glucosidase inhibitory effects of Sclerocarya birrea [(A. Rich.) Hochst.] subspecies caffra (Sond) Kokwaro (Anacardiaceae) stem-bark extracts. AJB 2011;10(66):15033-9. [DOI:10.5897/AJB11.1408]
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >

XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ojo O A, Ojo A B, Ajiboye B, Olayide I, Fadaka A. Helianthus annuus Leaf Ameliorates Postprandial Hyperglycaemia by Inhibiting Carbohydrate Hydrolyzing Enzymes Associated with Type-2 Diabetes . IJT. 2016; 10 (5) :17-22
URL: http://ijt.arakmu.ac.ir/article-1-505-en.html

Volume 10, Issue 5 (September-October 2016) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
Persian site map - English site map - Created in 0.051 seconds with 836 queries by yektaweb 3619