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Volume 11, Issue 5 (September-October 2017)                   IJT 2017, 11(5): 23-30 | Back to browse issues page

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Okoro P N, Ibrahim S, Inuwa H M, Okoduwa S I R. Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment. IJT. 2017; 11 (5) :23-30
URL: http://ijt.arakmu.ac.ir/article-1-557-en.html
1- Department of Basic Research, National Research Institute for Chemical Technology, Zaria, Nigeria. , peckyclett@sironigeria.com
2- Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
3- Department of Research and Development, Nigerian Institute of Leather and Science Technology, Zaria, Nigeria.
Abstract:   (3490 Views)
Background: The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seed kernel methanol extract on metalloproteases in Carpet Viper (Echis ocellatus) venom.
Methods: Mango seed kernel methanolic extract was evaluated in vitro for its anti-venom activity and inhibition of metalloproteases of Carpet Viper's (Echis ocellatus) venom. Metalloprotease portion was partially purified from the venom of E. ocellatus with a yield of 71%, a purification fold of 2.63 and a specific activity of 19.00 µmol/min/mg protein.
Results: The enzyme appeared as a band on SDS-PAGE with a molecular weight of 23 kDa. The kinetic properties of the enzyme showed a Km of 0.31 mg mL-1 and a Vmax of 9.09 µmol min-1. When the enzyme was incubated with the extract, kinetic studies revealed a mixed non-competitive pattern of inhibition with Km values of 0.56 and 1.11 mg mL-1 and Vmaxvalues of 6.67 and 4.17-µmol min-1 for 5% and 20% inhibitor concentrations, respectively. An estimated Ki value of 0.168 mg mL-1 was obtained from a secondary plot demonstrating that the extract had a high affinity for the partially purified enzyme; thus, could serve as an effective inhibitor.
Conclusion: Methanol extract of mango seed kernel has a high affinity for the partially purified enzyme, and it might provide an inexpensive and readily available alternative to sheep serum in the management of snakebite envenomation. Therefore, further in vivo studies are necessary to assess its effectiveness and safety.
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Type of Study: Research | Subject: General

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