Write your message
Volume 11, Issue 2 (March-April 2017)                   IJT 2017, 11(2): 15-19 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosseini Z, Khosravi M, Ghorbanpoor M, Mayahi M. Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice. IJT. 2017; 11 (2) :15-19
URL: http://ijt.arakmu.ac.ir/article-1-558-en.html
1- Student of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran.
2- Department of Pathobiology, Shahid Chamran University of Ahvaz, Iran. , m.khosravi@scu.ac.ir
3- Department of Pathobiology, Shahid Chamran University of Ahvaz, Iran.
4- Department of Clinical Sciences, Shahid Chamran University of Ahvaz, Iran.
Abstract:   (2305 Views)

Background: To explore the oral absorption of scorpion venom an ELISA were designed in this study. Scorpions and their venom were been used for centuries as medical treatments in traditional medicine. The oral administration of drug referred as the convenient way, as there was not any publication about gastro-intestinal absorption of scorpion venom; this experiment checked oral absorption of Mesobuthus eupeus scorpion venom in mice.

Methods: Six groups of mice orally received 0, 0.2, 0.5, 1, 2 and 5 mg/kg of M. eupeus venom and their blood samples were tacked after 15, 30, 60 min and 2, 4, 6, 24, 48 h after that. The presence of venom the blood samples were detected with a house- antigen capture ELISA.

Results: The venom was absorbed after its feeding to mice. The animals expressed no signs of envenomation and, the venom was detectable by AC-ELISA as soon as 15 min after its feed. Maximum serum levels were 2 h after its meal.

Conclusion: The orally administrated venom was absorbed to the blood circulation without any clinically symptoms.

Full-Text [PDF 132 kb]   (1156 Downloads)    
Type of Study: Research | Subject: General

1. Dunlop JA, Selden PA. Scorpion fragments from the Silurian of Powys, Wales. Arachnology 2013;16: 27–32. [DOI:10.13156/arac.2013.16.1.27]
2. Waddington J, Rudkin DM, Dunlop JA. A new mid-Silurian aquatic scorpion-one step closer to land? Biol Lett 2015;11(1):20140815. [DOI:10.1098/rsbl.2014.0815]
3. Karataş A. Mesobuthus eupeus (CL Koch, 1839)(Scorpiones: Buthidae) in Turkey. Anatolia. Euscorpius 2003; 7: 1–7.
4. Dehghani R, Rafinejad J, Fathi B, Panjeh-Shahi M, Jazayeri M, Hashemi A. A Retrospective Study on Scropionism in Iran (2002–2011). J Arthropod-Borne Dis 2016; 11(1): 309-18.
5. Eskandari G, Jolodar A, Shapouri MRS, Bahmainmehr A, Navidpour S. Production of Recombinant Alpha Neurotoxin of Scorpion Venom Mesobuthus eupeus and Analysis of its Immunogenicity. Iran Red Cres Med J 2014;16(1) :e9666-7. [DOI:10.5812/ircmj.9666]
6. Kuzmenkov AI, Vassilevski AA, Kudryashova KS, Nekrasova OV, Peigneur S, Tytgat J, et al. Variability of Potassium Channel Blockers in Mesobuthus eupeus Scorpion Venom with Focus on Kv1. 1 An integrated transcriptomic and proteomic study. J BiolChem 2015;290(19):12195-209. [DOI:10.1074/jbc.M115.637611]
7. Xu X, Duan Z, Di Z, He Y, Li J, Li Z, et al. Proteomic analysis of the venom from the scorpion Mesobuthus martensii. J proteomics 2014;106:162-80. [DOI:10.1016/j.jprot.2014.04.032]
8. Inceoglu B, Lango J, Wu J, Hawkins P, Southern J, Hammock BD. Isolation and characterization of a novel type of neurotoxic peptide from the venom of the South African scorpion Parabuthus transvaalicus (Buthidae). Eur J Biochem 2001;268(20):5407-13. [DOI:10.1046/j.0014-2956.2001.02479.x]
9. Cao Z, Di Z, Wu Y, Li W. Overview of scorpion species from China and their toxins. Toxins 2014;6(3):796-815. [DOI:10.3390/toxins6030796]
10. Ozkan O, Kat I. Mesobuthus eupeus scorpionism in Sanliurfa region of Turkey. J Venomous Anim Toxins Incl Trop Dis 2005;11(4):479-91. [DOI:10.1590/S1678-91992005000400008]
11. Theakston R, Warrell D, Griffiths E. Report of a WHO workshop on the standardization and control of antivenoms. Toxicon 2003;41(5):541-57. [DOI:10.1016/S0041-0101(02)00393-8]
12. Chippaux J-P, Goyffon M. Epidemiology of scorpionism: a global appraisal. Acta trop 2008;107(2):71-9. [DOI:10.1016/j.actatropica.2008.05.021]
13. Gomes A, Bhattacharjee P, Mishra R, Biswas AK, Dasgupta SC, Giri B, et al. Anticancer potential of animal venoms and toxins. Indian J Exp Biol 2010; 48(2): 93-103.
14. Khosravi M, Mayahi M, Jalali SM, Hosseini Z, Taghavi-Moghadam A, Hadinasab H. The resistance of mice and poultry to oral administration of scorpion venom. National congress of veterinary medicine in the service of community health and animal hygiene.2015.
15. Mikaelian A. Polarized scorpion venom solution and a method for making polarized scorpion venom solution. Google Patents; 2012.
16. Ding J, Chua P-J, Bay B-H, Gopalakrishnakone P. Scorpion venoms as a potential source of novel cancer therapeutic compounds. Exp Biol Med 2014;239(4):387-93. [DOI:10.1177/1535370213513991]
17. Chen C-X, Chen J-Y, Kou J-Q, Xu Y-L, Wang S-Z, Zhu Q, et al. Suppression of inflammation and arthritis by orally administrated cardiotoxin from Naja naja atra. J Evidence-Based Complementary Altern Med 2015;2015.
18. Giorgi R, Bernardi M, Cury Y. Analgesic effect evoked by low molecular weight substances extracted from Crotalus durissus terrificus venom. Toxicon 1993;31(10):1257-65. [DOI:10.1016/0041-0101(93)90399-4]
19. National Ethics Advisory Committee. Ethical Guidelines for Observational Studies: Observational research, audits and related activities. Revised edition. 2012.
20. March SC, Parikh I, Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem 1974;60(1):149-52. [DOI:10.1016/0003-2697(74)90139-0]
21. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5. [DOI:10.1038/227680a0]
22. Debatin K-M, Krammer PH. Death receptors in chemotherapy and cancer. Oncogene 2004;23(16):2950-66. [DOI:10.1038/sj.onc.1207558]
23. Heinen TE, Da Veiga ABG. Arthropod venoms and cancer. Toxicon 2011;57(4):497-511. [DOI:10.1016/j.toxicon.2011.01.002]
24. Ortiz E, Gurrola GB, Schwartz EF, Possani LD. Scorpion venom components as potential candidates for drug development. Toxicon 2015;93:125-35. [DOI:10.1016/j.toxicon.2014.11.233]
25. Pu X, Wong P, Gopalakrishnakone P. A novel analgesic toxin (hannalgesin) from the venom of king cobra (Ophiophagus hannah). Toxicon 1995;33(11):1425-31. [DOI:10.1016/0041-0101(95)00096-5]
26. Mitchell SW. Experimental contributions to the toxicology of rattle-snake venom: Moorhead, Simpson & Bond; 1868. [DOI:10.5962/bhl.title.51364]
27. Zhu K-Z, Liu Y-L, Gu J-H, Qin Z-H. Antinociceptive and anti-inflammatory effects of orally administrated denatured naja naja atra venom on murine rheumatoid arthritis models. J Evidence-Based Complementary Altern Med 2013;2013.
28. Malleswari M, Sschidevi P, Ravikanth SV, Josthna P, Jacob doss P. Oral toxicity study of the venom of Najanaja in albino rat. Int J Pharm Bio Sci 2014; 5(4): 935-41.
29. Dos Santos GGL, e Silva LLC, Soares MBP, Villarreal CF. Antinociceptive properties of Micrurus lemniscatus venom. Toxicon 2012;60(6):1005-12. [DOI:10.1016/j.toxicon.2012.07.003]
30. Malleswari M, Josthna P, Doss PJ. Orally Administered Venom of Naja Naja Alters Protein Metabolic Profiles in the Liver of Albino Rats. Int J Life Sci Biotechnol Pharma Res 2015;4(1):10.
31. Qin T, Yu L, Wang Z. Study on rectal mucosa absorption effects of cobra neurotoxins by I tracer labelling methods. J Zhejiang Coll TCM 2001;25(5):55-6.

Add your comments about this article : Your username or Email:

Send email to the article author

© 2020 All Rights Reserved | Iranian Journal of Toxicology

Designed & Developed by : Yektaweb