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Volume 12, Issue 2 (March-April 2018)                   IJT 2018, 12(2): 7-13 | Back to browse issues page

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Ghofrani Tabari D, Kermanshahi H, Golian A, Majidzadeh Heravi R. In Vitro Binding Potentials of Bentonite, Yeast Cell Wall and Lactic Acid Bacteria for Aflatoxin B1 and Ochratoxin A. IJT 2018; 12 (2) :7-13
URL: http://ijt.arakmu.ac.ir/article-1-642-en.html
In Vitro Binding Potentials of Bentonite, Yeast Cell Wall and Lactic Acid Bacteria for Aflatoxin B1 and Ochratoxin A
Damoon Ghofrani Tabari1 , Hassan Kermanshahi * 2, Abolghasem Golian1 , Reza Majidzadeh Heravi1
1- Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
2- Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. , hassbird@yahoo.com
Abstract:   (6451 Views)
Background: This study intended to assess individual and combined adsorption potentials of three adsorbents (processed bentonite as an inorganic adsorbent, and cell walls of Saccharomyces cerevisiae and of the GG strain of Lactobacillus rhamnosus as organic adsorbents) for aflatoxin B1 and ochratoxin A under in vitro conditions.
Methods: This study was conducted in Ferdowsi University of Mashhad, Mashhad, Iran in 2106. A pre-test with four treatments of bacteria and yeast (live or dead) with five replications was designed and conducted to study the effects of these biotic and abiotic organic adsorbents on toxin adsorption efficiency. The experiment in the main study had seven treatments including two toxins (2 ppm) and three adsorbents with five replications using the completely randomized design. Toxin quantities were measured by an HPLC instrument.
Results: The various types of dead organic adsorbents were more capable of adsorbing toxins compared to the live ones. The processed bentonite was considerably more efficient in adsorbing aflatoxin B1 (93.51) compared to the other treatment groups (P<0.05). No significant differences were observed between the effects of yeast and bacterial cell walls in adsorbing aflatoxin B1. However, the bacterial cell walls (61.71) had higher adsorption efficiencies in adsorbing ochratoxin A than the processed bentonite and yeast cell walls (P<0.05).
Conclusion: Processed bentonite (or montmorillonite) and bacterial cell walls are able to adsorb considerable quantities of aflatoxin B1and ochratoxin A, and can be used in multiple mycotoxin contaminations as an effective strategy for preventing or reducing the harmful effects of these toxins.
Keywords: Adsorbents, Bentonite, Detoxification, Lactobacillus, Mycotoxin, Yeast Cell Wall
Full-Text [PDF 1784 kb]   (2591 Downloads)    
Type of Study: Research | Subject: General
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