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Volume 14, Issue 4 (October 2020)
IJT 2020, 14(4): 201-214 |
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Larbie C, Emikpe B O, Oyagbemi A A, Jarikre T A, Adjei C O. Acalypha wilkesiana ‘Inferno’ Is Nephroprotective Against Gentamicin and Cisplatin: Biochemical, Histological and Immunohistochemical Evidence. IJT 2020; 14 (4) :201-214
URL: http://ijt.arakmu.ac.ir/article-1-821-en.html
URL: http://ijt.arakmu.ac.ir/article-1-821-en.html
Christopher Larbie * 
1, Benjamin O. Emikpe2 , Ademola A. Oyagbemi3 , Theophilus A. Jarikre4 , Clement O. Adjei1
1, Benjamin O. Emikpe2 , Ademola A. Oyagbemi3 , Theophilus A. Jarikre4 , Clement O. Adjei1
1- Department of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
2- Department of Pathobiology, School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
3- Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, Nigeria.
4- Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Nigeria.
2- Department of Pathobiology, School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
3- Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, Nigeria.
4- Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Nigeria.
Abstract: (2235 Views)
Background: Kidneys exposure to toxins can cause injuries, leading to their functional impairments. Traditionally, plants have been used for the treatment of renal disorders and numerous medicinal plants have been tested for their nephroprotective effects, in such cases as gentamicin (GM) and cisplatin (Cisp)-induced nephrotoxicity. This study assessed the ability of Acalypha wilkesiana’s extract to counteract its toxic effect based on the biochemical, histological and proinflammatory cytokines components in rats.
Methods: Thirty-six male Wistar rats were randomly divided into nine groups (n=4 each) and administered the following treatments: a) normal control (1 mL/kg body weight normal saline from days 1-10); b) nephrotoxin (GM 120 mg/kg, days 2-7; or Cisp 7 mg/kg on day 3); c) standard drug (120 mg/kg Silymarin plus GM or Cisp, days 1-10); and, d) extract groups (100 or 250 mg/kg, days 1-10 plus GM). Blood samples were collected and subjected to hematological and biochemical evaluations while kidney tissue samples were examined for histopathological alterations, pro- and antioxidants, and expression of pro-inflammatory cytokines.
Results: Treatment of the rats pre-exposed to GM or Cisp with the extract decreased the serum creatinine, urea and MDA levels. The GST and GPx levels were also restored in rats. Glomerular atrophy with tubular epithelial necrosis induced by either nephrotoxin was restored to near normal. The expression of COX-2 following the administration of either nephrotoxin was reversed after treatment with the extract.
Conclusion: The A. wilkesiana extract exhibited significant nephroprotective property, which could potentially be regarded as a promising alternative to the management of renal diseases.
Methods: Thirty-six male Wistar rats were randomly divided into nine groups (n=4 each) and administered the following treatments: a) normal control (1 mL/kg body weight normal saline from days 1-10); b) nephrotoxin (GM 120 mg/kg, days 2-7; or Cisp 7 mg/kg on day 3); c) standard drug (120 mg/kg Silymarin plus GM or Cisp, days 1-10); and, d) extract groups (100 or 250 mg/kg, days 1-10 plus GM). Blood samples were collected and subjected to hematological and biochemical evaluations while kidney tissue samples were examined for histopathological alterations, pro- and antioxidants, and expression of pro-inflammatory cytokines.
Results: Treatment of the rats pre-exposed to GM or Cisp with the extract decreased the serum creatinine, urea and MDA levels. The GST and GPx levels were also restored in rats. Glomerular atrophy with tubular epithelial necrosis induced by either nephrotoxin was restored to near normal. The expression of COX-2 following the administration of either nephrotoxin was reversed after treatment with the extract.
Conclusion: The A. wilkesiana extract exhibited significant nephroprotective property, which could potentially be regarded as a promising alternative to the management of renal diseases.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
