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Volume 10, Issue 4 (July-August 2016)
IJT 2016, 10(4): 13-20 |
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Hassanshahian M. Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils. IJT 2016; 10 (4) :13-20
URL: http://ijt.arakmu.ac.ir/article-1-487-en.html
URL: http://ijt.arakmu.ac.ir/article-1-487-en.html
Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran. , mshahi@uk.ac.ir
Abstract: (4701 Views)
Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran.
Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on amplifying of 16s rDNA.
Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environment. Three diazinon-degrading bacteria strains belong to: Pseudomonas putida strain D3, P. fluorescens strain D1 and Achromobacter piechaudii strain D8. The best degrading strain (D1), up to 100 ppm, illustrated a good growth, whereas more than this concentration, the growth was reduced. The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.
Conclusion: The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.
Using these strains and other biological reclamation methods we can eliminate bio-environmental problems.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
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