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:: Volume 10, Issue 4 (July-August 2016) ::
IJT 2016, 10(4): 13-20 Back to browse issues page
Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils
Mehdi Hassanshahian
Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran. , mshahi@uk.ac.ir
Abstract:   (1305 Views)

Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran.

Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on amplifying of 16s rDNA.

Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environment. Three diazinon-degrading bacteria strains belong to: Pseudomonas putida strain D3, P. fluorescens strain D1 and Achromobacter piechaudii strain D8. The best degrading strain (D1), up to 100 ppm, illustrated a good growth, whereas more than this concentration, the growth was reduced. The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.

Conclusion: The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.

Using these strains and other biological reclamation methods we can eliminate bio-environmental problems.

Keywords: Agricultural Pesticide, Degrading Bacteria, Diazinon
Full-Text [PDF 505 kb]   (987 Downloads)    
Type of Study: Research | Subject: Special
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Hassanshahian M. Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils. IJT. 2016; 10 (4) :13-20
URL: http://ijt.arakmu.ac.ir/article-1-487-en.html


Volume 10, Issue 4 (July-August 2016) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
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