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:: Volume 10, Issue 4 (July-August 2016) ::
IJT 2016, 10(4): 9-12 Back to browse issues page
Evaluation of some Enzymatic Changes in the Liver and Kidney of Rats Following Exposure to Sublethal Concentration of Potassium Cyanide
Hasan Baghshani * , Vahide Ghodsi
Department of Basic Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. , baghishani@ferdowsi.um.ac.ir
Abstract:   (1485 Views)

Background: Besides acute lethal cyanide poisoning, its chronic intoxication may also produce some pathologic effects on different tissues that precedes alterations in biochemical parameters. The present study was aimed to evaluate the effects of sublethal cyanide exposure on some tissue enzyme activities in liver and kidney of rats.

Methods: Twelve male rats were divided into two groups as follows: Group 1 rats served as control. Rats in group 2 received water containing 200 ppm inorganic cyanide. At the end of the experiment (42 days), hepatic and renal activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and rhodanese were measured.

Results: Potassium cyanide administration caused elevation of all measured liver enzymes in group 2, although the increase was only significant for AST and ALT activities as compared to control values (P<0.05). Moreover, renal AST activity in rats from group 2 was significantly higher than those from controls.

Conclusion: The altered tissue activities of some enzymes in the present study might reflect the metabolic disturbances due to cyanide intoxication in studied organs. However, further research should be focused on this issue for better understanding of the fine mechanism of cyanide effects upon metabolic enzyme activities.

Keywords: Cyanide Poisoning, Rat, Tissue Enzyme
Full-Text [PDF 44 kb]   (898 Downloads)    
Type of Study: Research | Subject: Special
References
1. Isom GE, Borowitz JL, Mukhopadhyay S. Sulfurtransferase enzymes involved in cyanide metabolism. In: Charlene A.M. editor. Comprehensive Toxicology. Oxford: Elsevier. 2010. pp 485–500. Downloaded from ijt.arakmu.ac.ir at 13:19 +0330 on Tuesday February 6th 2018 Iranian Journal of Toxicology Hasan Baghshani and Vahide Ghodsi 12 Vol 10, No 4 July-August 2016; http://www.ijt.ir
2. Shwetha A, Hosetti BB, Dube PN. Toxic Effects of Zinc Cyanide on Some Protein Metabolites in Fresh water fish, Cirrhinus mrigala (Hamilton). Int J Environ Res 2012; 6(3):769-78.
3. Wiemeyer SN, Hill AF, Carpenter JW, Krynitsky AJ. Acute oral toxicity of sodium cyanide in birds. J Wildlife Dis 1986; 22: 538–46. [DOI:10.7589/0090-3558-22.4.538]
4. Oruc HH, Yilmaz R, Bagdas D, Ozyigit MO. Cyanide poisoning deaths in dogs. J Vet Med A Physiol Pathol Clin Med 2006; 53(10): 509-10. [DOI:10.1111/j.1439-0442.2006.00892.x] [PMID]
5. Cliff J, Lundquist P, Rosling H, Sorbo B, Wide L. Thyroid function in a cassava-eating population affected by epidemic spastic paraparesis. Acta Endocrinologica 1986; 113: 523-8. [DOI:10.1530/acta.0.1130523]
6. Osuntokun BO. Cassava diet, chronic cyanide intoxication and neuropathy in Nigerian Africans. World Rev Nutr Diet 1981; 36: 141-73. [DOI:10.1159/000393156] [PMID]
7. Okolie NP, Iroanya CU. Some histologic and biochemical evidence for mitigation of cyanideinduced tissue lesions by antioxidant vitamin administration in rabbits. Food Chem Toxicol 2003; 41: 463–9. [DOI:10.1016/S0278-6915(02)00224-7]
8. Okolie NP, Asonye CC. Mitigation of cataractogenic potential of cyanide by antioxidant vitamin administration. J Med Biomed Res 2004;3(1): 48–52.
9. Okolie NP, Osagie AU. Liver and kidney lesions and associated enzyme changes induced in rabbits by chronic cyanide exposure. Food Chem Toxicol1999; 37: 745-50. [DOI:10.1016/S0278-6915(99)00059-9]
10. Sousa AB, Soto-Blanco B, Guerra JL, Kimura ET, Gorniak SL. Does prolonged oral exposure to cyanide promote hepatotoxicity and nephrotoxicity? Toxicology 2002; 174: 87-95. [DOI:10.1016/S0300-483X(02)00041-0]
11. Soto-Blanco B, Gorniak SL. Milk transfer of cyanide and thiocyanate: cyanide exposure by lactation in goats. Vet Res 2003; 34: 213-20. [DOI:10.1051/vetres:2002068] [PMID]
12. GholipourKanani H, Shahsavani D, Baghishani H. Effect of exposure to sublethal levels of potassium cyanide on serum and tissue enzymes in roach fish(Rutilus rutilus). Online J Vet Res 2013; 17(5):245-55.
13. Kaneko JJ, Harvey JW, Bruss ML. Clinical Biochemistry of Domestic Animals, 5th Ed,Academic Press, London.1999. p. 829-44.
14. Baghshani H, Shahsavani D. Effects of lead acetate exposure on metabolic enzyme activities in selected tissues of common carp (Cyprinus carpio). Comp Clin Pathol 2013; 22:903–7. [DOI:10.1007/s00580-012-1497-3]
15. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957; 25: 56–62. [DOI:10.1093/ajcp/28.1.56]
16. Burtis CA, Ashwood ER. Tietz textbook of clinical chemistry, 2nd Ed. Saunders, Philadelphia, 1994.p 625–888, 928–1081.
17. Sorbo B. Crystalline rhodanese: the enzyme catalyzed reaction. Acta Chemica Scandinavia 1953; 7: 1137–45. https://doi.org/10.3891/acta.chem.scand.07-0238 [DOI:10.3891/acta.chem.scand.07-1137]
18. Baghshani H, Aminlari M. Comparison of rhodanese distribution in different tissues of Japanese quail, partridge, and pigeon. Comp Clin Pathol 2009; 18: 217–20. [DOI:10.1007/s00580-008-0781-8]
19. Jarrar BM, Mahmoud ZN. Histochemical demonstration of changes in the activity of hepatic phosphatases induced by experimental lead poisoning in male white rats (Rattus norvegicus). Toxicol Ind Health 1999; 15: 1–9.
20. Rahman MF, Siddiqui MK. Biochemical effects of vepacide (from Azadirachta indica) on Wistar rats during subchronic exposure. Ecotoxicol Environ Saf 2004; 59(3): 332-9. [DOI:10.1016/j.ecoenv.2003.07.013] [PMID]
21. Al-Ghanim KA. Effect of cypermethrin toxicity on enzyme activities in the freshwater fish Cyprinus carpio. Afr J Biotechnol 2014. 13(10):1169–73. [DOI:10.5897/AJB12.1724]
22. Elsaid FG, Elkomy MM. Aqueous garlic extract and sodium thiosulphate as antidotes for cyanide intoxication in Albino rats. Res J Med Med Sci 2006; 1(2): 50–6.
23. Manzano H, Sousa AB, Soto-Blanco B, Guerra JL, Maiorka PC, Gorniak SL. Effects of long-term cyanide ingestion by pigs. Vet Res Commun 2007;31: 93–104. [DOI:10.1007/s11259-006-3361-x] [PMID]
24. Okolie NP, Osagie AU. Differential effects of chronic cyanide intoxication on heart, lung and pancreatic tissues. Food Chem Toxicol 2000; 38:543-8. [DOI:10.1016/S0278-6915(00)00020-X]
25. Sylvester DM, Sander CC. Immunohistochemicallocalization of rhodanese. Histochem J 1990;22:197–200. [DOI:10.1007/BF02386005]
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Baghshani H, Ghodsi V. Evaluation of some Enzymatic Changes in the Liver and Kidney of Rats Following Exposure to Sublethal Concentration of Potassium Cyanide . IJT. 2016; 10 (4) :9-12
URL: http://ijt.arakmu.ac.ir/article-1-491-en.html


Volume 10, Issue 4 (July-August 2016) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
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