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Volume 11, Issue 6 (November-December 2017)
IJT 2017, 11(6): 1-5 |
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Ajao M S, Sansa A B, Imam A, Ibrahim A, Adana M Y, Alli-Oluwafuyi A et al . Protective Effect of Nigella Sativa (Black Caraway (Oil on Oral Dichlorvos Induced Hematological, Renal and Nonspecific Immune System Toxicity in Wistar Rats. IJT 2017; 11 (6) :1-5
URL: http://ijt.arakmu.ac.ir/article-1-591-en.html
URL: http://ijt.arakmu.ac.ir/article-1-591-en.html
Moyosore Salihu Ajao1 
, Adebayo Babatunde Sansa1 , Aminu Imam * 2, Abdulmumin Ibrahim1 , Misturat Yetunde Adana1 , Abdulmusawwir Alli-Oluwafuyi3 , Suwebat Bidemi Kareem1
, Adebayo Babatunde Sansa1 , Aminu Imam * 2, Abdulmumin Ibrahim1 , Misturat Yetunde Adana1 , Abdulmusawwir Alli-Oluwafuyi3 , Suwebat Bidemi Kareem1
1- Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria.
2- Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria. ,imam.a@unilorin.edu.ng
3- Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria.
2- Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria. ,
3- Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria.
Abstract: (6819 Views)
Background: Exposure to environmental toxins such as organophosphates poses a great threat to the health of the public. In this work, we investigated the effects of continuous exposure to dichlorvos (DDVP) on kidney function and hematological parameters, and the possible antidote activity of Nigella sativa oil (NSO).
Methods: This research was conducted in 2016, at The Animal Holding and Research Laboratory of Faculty Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria. Twenty-four Wistar rats were randomly divided into four groups, six rats each. The four groups received: 1. phosphate buffer solution as controls, 2. DDVP, 3. DDVP+NSO and 4. NSO alone. After 2 wk of treatment, blood samples were collected and hematological profile (RBC, Hb), erythrocyte indices (MCV, MCH, MCHC, and Plt), renal function parameters (albumin, urea, total protein, chloride, sodium, and potassium ions) and nonspecific immune response (WBC) were measured.
Results: Rat exposed to DDVP showed red blood cell count, hemoglobin, packed cell volume, albumin, and total protein levels was reduced from control, while white blood cell count and urea significantly increased as compared to controls, the change in K+ level was not significant. NSO maintained optimal levels of red blood cell count, hemoglobin, packed cell volume, albumin, white blood cell count, and urea, indicative of its protective effect against hemo-, immuno- and nephrotoxicity of DDVP.
Conclusion: N. sativa (Black Caraway) oil might be a potential antidote in hematotoxicity, immunosuppression and renal dysfunction in organophosphate poisoning, especially dichlorvos. The protective effect of NSO against dichlorvos toxicity can be attributed to its antioxidant capacity.
Methods: This research was conducted in 2016, at The Animal Holding and Research Laboratory of Faculty Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria. Twenty-four Wistar rats were randomly divided into four groups, six rats each. The four groups received: 1. phosphate buffer solution as controls, 2. DDVP, 3. DDVP+NSO and 4. NSO alone. After 2 wk of treatment, blood samples were collected and hematological profile (RBC, Hb), erythrocyte indices (MCV, MCH, MCHC, and Plt), renal function parameters (albumin, urea, total protein, chloride, sodium, and potassium ions) and nonspecific immune response (WBC) were measured.
Results: Rat exposed to DDVP showed red blood cell count, hemoglobin, packed cell volume, albumin, and total protein levels was reduced from control, while white blood cell count and urea significantly increased as compared to controls, the change in K+ level was not significant. NSO maintained optimal levels of red blood cell count, hemoglobin, packed cell volume, albumin, white blood cell count, and urea, indicative of its protective effect against hemo-, immuno- and nephrotoxicity of DDVP.
Conclusion: N. sativa (Black Caraway) oil might be a potential antidote in hematotoxicity, immunosuppression and renal dysfunction in organophosphate poisoning, especially dichlorvos. The protective effect of NSO against dichlorvos toxicity can be attributed to its antioxidant capacity.
Keywords: Anemia, Dichlorvos, Kidney Function Tests, Nigella Sativa, Organophosphates, Organophosphate Poisoning
References
1. Lokke H, Ragas AM, Holmstrup M. Tools and perspectives for assessing chemical mixtures and multiple stressors. Toxicol 2013;313(2):73-82. [DOI:10.1016/j.tox.2012.11.009]
2. Zaluski R, Kadri SM, Alonso DP, Martins Ribolla PE, de Oliveira Orsi R. Fipronil promotes motor and behavioral changes in honey bees (Apis mellifera) and affects the development of colonies exposed to sublethal doses. Environ Toxicol Chem 2015;34(5):1062-9. [DOI:10.1002/etc.2889]
3. Abdel-Daim MM. Synergistic protective role of ceftriaxone and ascorbic acid against subacute diazinon-induced nephrotoxicity in rats. Cytotechnology 2016;68(2):279-89. [DOI:10.1007/s10616-014-9779-z]
4. Li S, Cao C, Shi H, Yang S, Qi L, Zhao X, et al. Effect of quercetin against mixture of four organophosphate pesticides induced nephrotoxicity in rats. Xenobiotica 2016;46(3):225-33. [DOI:10.3109/00498254.2015.1070443]
5. Mehri N, Felehgari H, Harchegani A, Behrooj H, Kheiripour N, Ghasemibasir H, et al. Hepatoprotective effect of the root extract of green tea against malathion-induced oxidative stress in rats. J Herb Med Pharmacol 2016;5:116-9.
6. Deka S, Mahanta R. Dichlorvos toxicity on fish-a review. Eur J Bio Res 2015;5(3):78-85.
7. Brown H, Oruambo F, Kenanagha B. Poor anted effects of copper and manganese on ratsexpossed to acute dose of dichlorvos. Ejpmr 2015; 2(1):290-303.
8. Yadav P, Jadhav S, Kumar V, Kaul K, Pant S, Flora S. Protective efficacy of 2-PAMCl, atropine and curcumin against dichlorvos induced toxicity in rats. InterdiscToxicol 2012;5(1):1-8. [DOI:10.2478/v10102-012-0001-x]
9. Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Tropic Biomed 2013;3(5):337-52. [DOI:10.1016/S2221-1691(13)60075-1]
10. Ahmad S, Beg ZH. Elucidation of mechanisms of actions of thymoquinone-enriched methanolic and volatile oil extracts from Nigella sativa against cardiovascular risk parameters in experimental hyperlipidemia. Lipids Health Dis 2013;12(1):86-7. [DOI:10.1186/1476-511X-12-86]
11. Ahmed MA, Hassanein KM. Cardio protective effects of Nigella sativa oil on lead induced cardio toxicity: Anti inflammatory and antioxidant mechanism. J Physiol Path 2013;4(5):72-80. [DOI:10.5897/JPAP2013.0083]
12. Bai T, Lian L-H, Wu Y-L, Wan Y, Nan J-X. Thymoquinone attenuates liver fibrosis via PI3K and TLR4 signaling pathways in activated hepatic stellate cells. Int Immunopharmacol 2013;15(2):275-81. [DOI:10.1016/j.intimp.2012.12.020]
13. Narra MR. Haematological and immune upshots in Clarias batrachus exposed to dimethoate and defying response of dietary ascorbic acid. Chemosphere 2017;168:988-95. [DOI:10.1016/j.chemosphere.2016.10.112]
14. Narra MR. Single and cartel effect of pesticides on biochemical and haematological status of Clarias batrachus: A long-term monitoring. Chemosphere 2016;144:966-74. [DOI:10.1016/j.chemosphere.2015.09.065]
15. Sharma P, Singh R. Dichlorvos and lindane induced oxidative stress in rat brain: Protective effects of ginger. Pharm Res 2012;4(1):27-8.
16. Korany NS, Ezzat BA. Prophylactic effect of green tea and Nigella sativa extracts against fenitrothion-induced toxicity in rat parotid gland. Arch Oral Biology 2011;56(11):1339-46. [DOI:10.1016/j.archoralbio.2011.05.006]
17. Jordaan MS, Reinecke SA, Reinecke AJ. Biomarker responses and morphological effects in juvenile tilapia Oreochromis mossambicus following sequential exposure to the organophosphate azinphos-methyl. AquatToxicol 2013;144:133-40. [DOI:10.1016/j.aquatox.2013.10.007]
18. Jorum O, Piero N, Machocho A. Haematological Effects of Dichloromethane-Methanolic Leaf Extracts of Carissa edulis (Forssk.) Vahl in Normal Rat Models. J Hematol Thrombo Dis 2016;4(232):2-3. [DOI:10.4172/2329-8790.1000232]
19. Kanu KC, Ijioma SN, Atiata O. Haematological, Biochemical and Antioxidant Changes in Wistar Rats Exposed to Dichlorvos Based Insecticide Formulation Used in Southeast Nigeria. Toxics 2016;4(4):28-9. [DOI:10.3390/toxics4040028]
20. Narra MR. Single and cartel effect of pesticides on biochemical and haematological status of Clarias batrachus: A long-term monitoring. Chemosphere 2016;144:966-74. [DOI:10.1016/j.chemosphere.2015.09.065]
21. Rehman H, Aziz AT, Saggu S, VanWert AL, Zidan N, Saggu S. Additive toxic effect of deltamethrin and cadmium on hepatic, hematological, and immunological parameters in mice. Toxicol Ind Health 2017;33(6):495-502. [DOI:10.1177/0748233716684710]
22. Chakroun S, Ezzi L, Grissa I, Kerkeni E, Neffati F, Bhouri R, et al. Hematological, biochemical, and toxicopathic effects of subchronic acetamiprid toxicity in Wistar rats. Environ Sci Pollut Res 2016;23(24):25191-9. [DOI:10.1007/s11356-016-7650-9]
23. Gh Farag A, M Gamila A, Kotb, Hamza A, Hussein R, Elhalwagy M. Impact of Organophosphorus insecticide Triazophos on Liver, Kidneys and Thyroid in Albino Rats. Int J Adv Res Biol Sci 2016; 3:199–208.
24. Nasr HM, El-Demerdash FM, El-Nagar WA. Neuro and renal toxicity induced by chlorpyrifos and abamectin in rats. Environ Sci Pollut Res 2016;23(2):1852-9. [DOI:10.1007/s11356-015-5448-9]
25. Kanbur M, Siliğ Y, Eraslan G, Karabacak M, Sarıca ZS, Şahin S. The toxic effect of cypermethrin, amitraz and combinations of cypermethrin-amitraz in rats. Environ Sci Pollut Res 2016;23(6):5232-42. [DOI:10.1007/s11356-015-5720-z]
26. Farooqui Z, Afsar M, Rizwan S, Khan AA, Khan F. Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on membrane enzymes, carbohydrate metabolism and oxidative damage in rat liver. Toxicol Rep 2016;3:328-35. [DOI:10.1016/j.toxrep.2016.02.004]
27. Mohtashami A, Entezari MH. Effects of Nigella sativa supplementation on blood parameters and anthropometric indices in adults: A systematic review on clinical trials. Journal of research in medical sciences: the official Isfahan J Res Med Sci 2016;21:3-4. [DOI:10.4103/1735-1995.175154]
28. Mosbah R, Yousef MI, Maranghi F, Mantovani A. Protective role of Nigella sativa oil against reproductive toxicity, hormonal alterations, and oxidative damage induced by chlorpyrifos in male rats. Toxicol Ind Health 2016;32(7):1266-77. [DOI:10.1177/0748233714554675]
29. Farooqui Z, Ahmed F, Rizwan S, Shahid F, Khan AA, Khan F. Protective effect of Nigella sativa oil on cisplatin induced nephrotoxicity and oxidative damage in rat kidney. Biomed Pharmacother 2017;85:7-15. [DOI:10.1016/j.biopha.2016.11.110]
30. Hosseinian S, Rad AK, Mousa-Al-Reza Hadjzadeh NM, Roshan SH, Shafiee S. The protective effect of Nigella sativa against cisplatin-induced nephrotoxicity in rats. Avicenna J Phytomed 2016;6(1):44-5.
31. Dhouib IE-B. Anti-inflammatory Effects of N-acetylcysteine against Carbosulfan-induced Hepatic Impairment in Male Rats. Recent Adv Biol med 2015; 1:29-40. [DOI:10.18639/RABM.2015.01.156935]
32. Nurulain SM, Ojha S, Tekes K, Shafiullah M, Kalasz H, Adem A. Efficacy of N-acetylcysteine, glutathione, and ascorbic acid in acute toxicity of paraoxon to Wistar rats: survival study. Oxid Med Cell Longevity 2015;2015.
33. Manal EA, Elhalwagy AA, Nahas RMZ, Hoda EAF. Potential Effect of Vitamin C and Curcumin on Oxidative Stress and Skin Lesion Induced by Dermal Intoxication with Cypermethrin. Amer Chem Sci J 2015; 8(1):1-12. [DOI:10.9734/ACSJ/2015/17285]
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